Dissertation > Excellent graduate degree dissertation topics show

Interaction of TFPI-2 with RASSF1C and Role of SOK1 on Breast Cancer Cell Migration

Author: ChenXuDong
Tutor: MaDuan
School: Fudan University
Course: Molecular Medicine
Keywords: Yeast two-hybrid Tissue factor pathway inhibitor -2 RASSF1C Protein interactions YSK1 (SOK1)
CLC: R737.9
Type: PhD thesis
Year: 2010
Downloads: 64
Quote: 0
Read: Download Dissertation

Abstract


Tissue factor pathway inhibitor -2 (tissue factor pathway inhibitor-2 of TFPI-2), also known as placental protein -5 (placenta protein-5, PP-5) and matrix-associated serine protease inhibitor (matrix associated serine protease inhibitor, MSPI ) is a protease inhibitor with kunitz type domains belong to the serine protease inhibitor superfamily members. Mature TFPI-2 by the N-terminal acidic amino acid-rich, the three series kuntiz domain and the C-terminal basic amino acid-rich composition, can inhibit trypsin, plasmin, chymotrypsin slow plasma kallikrein the activity of the enzyme, cathepsin G, and a variety of matrix metalloproteinases (MMPs), thus combating the invasion and metastasis of various malignant tumors. Similar TFPI-2 with the same family of tissue factor pathway inhibitor -1 (TFPI-1), part of the same functions, is also an important anticoagulant, coagulation factor VIIa (FVIIa) / tissue factor (tissue factor, TF ) complex and coagulation factor Xa (FXa), etc. have different inhibition. But so far, the physiological function of TFPI-2 is unclear of TFPI-2 interaction with other proteins known limited. We use Matchmaker 3 yeast two-hybrid system, to build a pGBKT7/TFPI-2 plasmid vector. This plasmid as bait, 95 protein-coding genes from the human fetal brain cDNA library screening, including 32 metabolism-related genes (eight of them involved in protein metabolism, involved in nucleic acid metabolism, 11 involved in glucose metabolism) 13, 21 a signal transduction genes, five cell growth and survival-related gene, seven transport-related genes, two anti-apoptotic gene, the function of the remaining 28 genes is not clear. By analyzing the cellular localization of relevant proteins of known function and library vectors in the two-hybrid system, the correct expression of the possibility of further identified eight potential interaction may TFPI-2 protein. On the basis of this study, we selected two proteins RASSF1C and ASB, in order to determine their interaction with TFPI-2 exists. ASB proteins in successfully copy genes, 36 positive clones were no target band was detected by PCR. Therefore, we will study the focus in determining whether TFPI-2 RASSF1C whether there is interaction. We use different colored fluorescent expression vectors were constructed TFPI-2 expression vector (fusion expression) RASSF1C laser scanning confocal microscopy found that there existed co-localization, co-immunoprecipitation method to determine TFPI-2 with RASSF1C exist the interaction . Serine / threonine protein kinase Ste20 family involved in multiple signaling pathways, including participation in the control of cell migration and polarity of the signal path. The Ste20 family can be divided into p21-activated protein kinase (p21-activated kinases) and germinal center kinase (germinal center kinases, GCK) two subfamilies. GCK can be further divided into eight subsets, 2 and 3 subsets the the mammalian Ste20 (mst) protein kinase MST1, MST2 investigate the regulation of MST3, MST4 and yeast Spsl/Ste20 associated kinase 1 (YSK1 mammalian homolog SOK1). YSK1 also been referred to as the Ste20 oxidative stress reactive protein kinase 1, reactive oxygen intermediates can be frail activated, but not by any other environmental stress, or growth factor activation. However, the kinase is not involved in any known protein kinase (MAPK) pathway, and YSK1 physiological function is unknown. The same YSK1, MST4 overexpression can not activate the JNK and p38 kinase pathway, but can promote non-anchor-dependent proliferation and tumor formation, and is associated with prostate cancer progression. The the MST series kinase YSK1, and MST4 Golgi matrix protein GM130 presence of signal contact, which play a role in the control of cell migration and polarization. Has found YSK1, and MST4 located in the Golgi apparatus, YSK1, and MST4 Golgi matrix protein GM130 interacts. MST4 cell migration. We find YSK1 with MST3 interact the, MST3 affect cancer cell migration. We adopt the method the cell sok1 knockout, to confirmed the sokl involved in breast cancer cell migration.

Related Dissertations

  1. Expression of AQP4, TFPI2, CaM Protein in Different Stages of Human Cervical Lesions of Uygur,R737.33
  2. Sub-cellular Localization of APOBEC-3F and-3G and Their Interaction on Hepatitis B Core Antigen,R512.62
  3. NYD-SP14 gene function,Q75
  4. Function Analysis of BL0033 and BL0034 in Fructose ABC Transporter System of Bifidobacterium Longum,Q935
  5. Predictions of Human Kinome-Small Molecule Interactions,Q55
  6. Development and Evaluation of Time-resolved Fluorescence Immunoassay for Tissue Factor Pathway Inhibitor-2 (TFPI-2),R446.6
  7. Studies on the in Vivo Interaction between MD-2 and TLR4 Based on FRET,R459.7
  8. Functional Analysis and Characterization of OsEF1 in Rice (Oryza Sativa L. ) Early Flowering Mutant,S511
  9. Research on the Fate of Estrogencity in Urban Water Cycing of Harbin,X52
  10. TFPI-2 in the pathogenesis of preeclampsia role,R714.245
  11. A Chip-based Coimmunoprecipitation Technique for Analysis of Protein-protein Interactions,R446.6
  12. Screening of Classical Swine Fever Virus NS2 Interaetive Proteins in Swine Umbilical Vein Endothelial Cell Line by Yeast Two-hybrid,S858.28
  13. Screening and Analysising New Binding Protein （Nbp）, a Novel Interactive Protein with p12CDK2AP1 , by Yeast Two-hybird Technique and Bioinformatics,Q78
  14. Preliminary Research of Function and Differentiation Regulating Mechanism of RAI 16 Protein Interacting with Tec Tyrosine Kinase,R73-3
  15. Isolation, Identification and Bioinformatics Analysis of Intestinal Trefoil Factor Receptor,Q51
  16. Preliminary Analysis on Function of CK1A Gene in Arabidopsis,Q943
  17. Prediction of Protein-protein Interaction and DNA Methylation Sites Based on Increment of Diversity Combined with Quadratic Discriminant Analysis,Q51
  18. Confirmation and Functional Exploration of the Interaction between Angiogenin and Fibulin Protein Family,R735.3
  19. Study on Law and Predicting Methods of Protein-protein Interaction,Q51
  20. Screening Molecular Targets of Phytophthora Sojae RXLR Effectors,S435.651
  21. The Interaction of OsJAB1 and OsVTC1 in Rice,S511

CLC: > Medicine, health > Oncology > Genitourinary tumors > Breast tumor
© 2012 www.DissertationTopic.Net  Mobile