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The Experimental Study of Baihui-Qubin Acupuncture on Effects of MMP-2、CYTC、VCAM、NGB after Acute Intracerebral Hemorrhage in Rats

Author: ZhangXiaoZhong
Tutor: ZouWei
School: Heilongjiang University of Traditional Chinese Medicine
Course: Traditional Chinese Medicine
Keywords: Acupuncture Cerebral hemorrhage The acupuncture of BaiHui-QuBin MMP-2mRNA NGBmRNA
CLC: R245
Type: PhD thesis
Year: 2011
Downloads: 137
Quote: 0
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Abstract


Objective:Observe the expression of MMP-2、NGB、CytC and VCAM-1 protein inintracerebral hemorrhage in rat brain through "Baihui" Acupuncture "QuBin" to explore the brain tissue Neuroprotective effects and possible mechanisms to provide experimental basis for clinical application.Methods:SD male rats were first randomly divided into three groups:sham operation groups, model groups and the acupuncture groups, each group was divided after 6h, 1d,2d,3d, 7d, every point included 10 rats. Preparation of autologous blood injection ICH model through the use of acupuncture Baihui QuBin hole in the head through the top three frontal and temporal area,using a needle through the multi-point method of treatment.Using Berderson to estimate neurological signs of nerve behavior change; wet and dry weight method for determination of brain tissue water content; observing pathological changes in brain tissue; MMP-2, NGB, CytC and VCAM-1 protein expression were detected by immunohistochemistry in brain tissue; by in situ hybridization detection of brain tissue MMP-2, NGBmRNA gene expression.Results:1.Under the light microscope, in the acupuncture group,the results of brain tissue pathological changes compared with model group changed significantly.2.The acupuncture group at each time point signs of neurological deficit in rats significantly restored, compared with the model group, 1d,2d,3d,7d had significant differences, with statistical significance, P<0.05 or P<0.01.3. Acupuncture group at each time point brain water content was less than model group at corresponding time points, compared with model group, 1d,2d,3d,7d was statistically significant, P<0.05 or P<0.01.4. Model group at different time points CytC protein expression increased over time, 1d reached the peak, then decreased gradually,7d, remains higher than the sham group with sham operation was statistically significant, P<0.01. Acupuncture treatment, at each time point CytC rat brain protein expression was significantly reduced, and compared the model group at the same time, was statistically significant, P<0.01.5. Model group at different time points the expression of VCAM-1 protein enhanced the growth over time,2d reached the peak, then decreased gradually,7d, remains higher than the sham group with sham operation was statistically significant, P<0.01. Acupuncture treatment, at each time point in rat brain expression of VCAM-1 protein was significantly reduced, and the same time the model group, was statistically significant, P<0.01.6. Model group at each time point MMP-2 protein and MMP-2 mRNA expression increased, 1d reached the peak, then decreased gradually,7d, remains higher than the sham group, compared with the sham operation was statistically significant, P<0.01. Acupuncture treatment, at each time point in rat brain tissue MMP-2 protein and gene expression of MMP-2mRNA significantly lower, with the same time the model group, was statistically significant, P<0.01.7. Model group at different time points and NGBmRNA NGB protein expression increased, Id reached the peak, then decreased gradually,7d, remains higher than the sham group with sham operation was statistically significant, P<0.01. Acupuncture treatment, at each time point in the NGB protein in rat brain gene expression and NGBmRNA significantly increased, at the same time the model group, was statistically significant, P<0.01. Conclusion:1. "Baihui" through "QuBin" acupuncture can significantly reduce brain water content, reduce the brain edema and improve the state of brain tissue pathological damage, thereby improving cerebral hemorrhage in different time symptoms of neurological damage.2."Baihui" through "QuBin" Acupuncture can inhibit CytC during intracerebral hemorrhage in rat brain, reduce neuronal apoptosis.3."Baihui" through "QuBin" Acupuncture can inhibit intracerebral hemorrhage in rat brain expression of VCAM-1, to ensure the integrity of brain vascular endothelial cells, reducing the inflammatory cascade caused by cerebral edema.4."Baihui" through "QuBin" Acupuncture can inhibit intracerebral hemorrhage in rat brain MMP-2 protein and MMP-2 mRNA gene expression, thereby reducing cerebral hemorrhage degradation on the basement membrane and extracellular matrix5."Baihui" through "QuBin" Acupuncture can improve the rat brain hemorrhage NGBmRNA NGB protein and gene expression, to improve cerebral ischemia, hypoxia, and thus play a protective role in nerve cells.

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