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The Experimental Study on Hydrogen Production of Several Varieties Spirulina (Arthrospira)

Author: LiQuanShun
Tutor: XuChengHai
School: Northeastern University
Course: Fluid Machinery and Engineering
Keywords: Spirulina Arthrospira algae Spirulina subsalsa Hydrogen Ultrastructure
CLC: TQ116.2
Type: PhD thesis
Year: 2009
Downloads: 57
Quote: 0
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Abstract


Spirulina as a natural food and medicines has been accepted, its nutritional value and medicinal value has been the practice proved. Spirulina other features of the development and research in recent years began to receive attention, especially from 1979, found that it also has since desorption characteristics of spirulina as a bioenergy research even more attention has been particularly popular. Into the 21st century, due to the energy crisis, environmental pollution and the greenhouse effect and destruction of the ecological environment, making more urgent need for the development and research of bio-energy research on spirulina hydrogen more and more attention by researchers. Because Spirulina not only as foods and pharmaceuticals, is also developed to extract other substances can be used as raw materials. Spirulina can release hydrogen storage as you can use hydrogen collected, will be the ideal biological found clean, renewable energy. Spirulina put hydrogen research has important practical applications of significance. Spirulina is a photosynthetic capacity of autotrophic prokaryotic microorganisms, hydrogen is a physiological phenomenon Spirulina specific hydrogen generation is closely related to physiological processes and growth, and the entire process is completed in Spirulina cells 's. Spirulina growth rely on its cell photosynthesis Spirulina photosynthesis system by PSI (light system I) and PS Ⅱ (light system Ⅱ), both of which exist in the the Spirulina cells within class thylakoid film, and in the light conditions, the PS Ⅱ absorb energy, the decomposition of water to generate an electron, receiving electronic through the cells in the electron acceptor, electron transfer to the electron transfer chain to the PSI electron acceptor, Zaishu sent to the ferredoxin (Fd ) when catalase obtained from ferredoxin electronic conveyed by the electron transport chain, the H ions are reduced to hydrogen released, thereby generating the hydrogen desorption phenomenon. Therefore, spirulina hydrogen in the role of catalase and physiological processes of growth are closely linked. Spirulina hydrogen production is limited to basic research experiments. We selected nine strains of Spirulina (varieties), divided by biological classification perspective, eight strains of Arthrospira algae belong to a species that salt marshes the Spirulina belonging Spirulina case. Indoor under normal culture conditions, a detailed experimental study of their hydrogen desorption characteristics rich the experimental basic information about Spirulina put hydrogen research, both in experimental technology or mechanism of Spirulina put hydrogen have important significance. Using gas chromatography and OD560 value determination method, the detection of nine kinds of of Spirulina hydrogen activity and growth rate, and the experimental results were compared and analyzed. Normal culture conditions, indoors, select the light intensity of the impact Spirulina hydrogen activity and growth rate of the major environmental factors, exposure time, incubation temperature, the oscillation frequency, inoculum size single factor experimental studies. Through measurement result of the discharge of the hydrogen activity and growth rate analysis showed that the hydrogen activity and growth rate of the nine kinds spirulina (Arthrospira algae), also showed growth on the environmental requirements of different culture conditions in the various environmental factor requirement broad differences. Grown for eight days to select the most suitable for the growth of the environmental factors of various algae species culture conditions, when the number of transferred dark culture assays of its hydrogen activity, comparing the hydrogen activity size, from the experimental results show that different algae species performance hydrogen activity a different order:, gt; No. 9, No. 8 gt; No. 4 gt; No. 1 gt; No. 7 gt; No. 2 gt; No. 5 gt; No. 3 gt; No. 6. Measured growth rate also showed different results, the following order: No. 9 gt; No. 2 gt; No. 4 gt; No. 8 gt; No. 7 gt; No. 5 gt; No. 3 gt; No. 1 gt; 6. This shows that the hydrogen desorption and growth among different strains of Spirulina (varieties) are significantly different, but basically positive correlation between the hydrogen activity and growth rate between, but it is not a one-to-one relationship. Optimized by orthogonal design method, the main environmental factors of the hydrogen activity put on the impact 9 Spirulina cultivation process conditions optimization experiments, each Spirulina hydrogen optimal culture conditions, affecting put hydrogen activity mainly factor. The experiments show that each of Spirulina discharge hydrogen optimized culture conditions are different, terms of its impact of primary and secondary relationship, different from the performance in different strains (varieties). However, the overall results of analysis, light intensity and oscillation frequency is the main factor to influence the Spirulina put hydrogen activity. The reason for the influence of the light intensity on the Spirulina photosynthesis, thereby affecting the photosynthesis of the accumulation of energy, and the energy is much directly affects the the hydrogen activity level; oscillation frequency is the dissolved oxygen concentration in the culture medium by regulating the level to affect the efficiency of photosynthesis, and ultimately also affect the accumulation of energy, thereby affecting the hydrogen activity. Spirulina put the results of the optimization experiments between the hydrogen activity with environmental factors to do further analysis and calculation, find out the regression equation between the to put hydrogen activity and environmental factors, and thus obtained Spirulina put the hydrogen activity of the environmental The quantitative relationship between the factor To explore the study of of Spirulina hydrogen conditions of the experimental data. The Spirulina has put hydrogen activity each hydrogen activity and release hydrogen time curve can be drawn through the experimental determination, and then calculate the hydrogen activity and release hydrogen regression equation of time, processing obtained by the integral The amount of hydrogen, with the discharge of hydrogen time computation relation formula, thereby realizing each Spirulina production method of the calculation of the amount of hydrogen in the hydrogen desorption unit time. Based on the nine kinds of Spirulina hydrogen activity and put the Comparative Study of the Determination of the amount of hydrogen obtained different spirulina species with different hydrogen activity and put the amount of hydrogen, but also quite different. The Spirulina subsalsa put the the maximum hydrogen activity, the activity of 98 nmol · mg-1dry-wt · h-1, the amount of hydrogen production is also up wt to 10132.954 nmol · m-1dry. Different strains (varieties) there is a difference in the growth rate, the growth is the 9th fastest Spirulina subsalsa followed No. 2 Arthrospira algae. Therefore, the study of spirulina hydrogen, algae species selection is very important. Using light microscopy and electron microscopy observation of Spirulina hydrogen cell biology experimental research. By observations on the the spirulina algae wire external morphology and ultrastructure, access to each Spirulina Filaments surface morphology and the internal structure of the microscopic structure diagram, found that there are differences between the different algae species. Combination of Spirulina the hydrogen growth, analysis and comparison of the microstructure of different algae species found fast growth and put the amount of hydrogen algae species contain the thylakoid carboxylation body cells containing material, in particular, is to put the maximum amount of hydrogen Spirulina subsalsa relative to the the Arthrospira algae increased significantly. This description of the different the thylakoid and carboxylation body content directly affect the strength of its photosynthesis efficiency, Spirulina photosynthesis directly affect the speed of growth, but also affect the efficiency of hydrogen. Response the Spirulina put the close relationship between the hydrogen and cell structure and cell physiological functions. That different algae species exist experimental study of nine kinds of Spirulina put hydrogen desorption amount of experimental data, so as to put hydrogen spirulina algae species selection, but also provide a basis for Spirulina algae species screening and method. Spirulina cell surface structure and ultrastructural features of the determination and observation show that the the different desorption of algae species there are differences, but also to the in-depth study of spirulina hydrogen production mechanism of cell biology provides basic information on the molecular level explore the relationship between the discharge the hydrogen mechanism and the mechanism of photosynthesis, and both have important practical and theoretical significance.

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CLC: > Industrial Technology > Chemical Industry > Basic inorganic chemical industry > Industrial gases > Hydrogen
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