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Objective To study the effects of aerobic exercise and aerobic exercise+resistance exercise on serum RBP4 in overweight/obesity young man.To discuss the relationship between RBP4 and body mass, body fat percent,blood lipid,body mass index,insulin resistance index,waist circumferenc,waist-hip ratio and V02max before and after exercise prescription.To research the change law and than to provide theory foundation that exercise presciption could improve insulin resistance through the influence in RBP4.To study the effects of aerobic exercise on glucose intake,expression and phosphorylation of insulin receptor and insulin receptor substrate1 in skeletal muscle,adipose tissue and liver of RBP4-induced insulin resistance rats.To study the effects of aerobic exercise on expression of phosphoric acid inositol-3-kinase and protein tyrosine phosphatase 1B in RBP4-induced insulin resistance rats skeletal muscle.To study the effects of aerobic exercise on hepatic glycogen, expression of glucokinase and phosphoenolpyruvate carboxykinase in RBP4-induced insulin resistance rats liver.To proclaim the mechanism that exercise improve RBP4-induced insulin resistance.Method According to BMI,ninety-three overweight/obesity university students were enroled as standard objects.Objects consist of forty-five fale and forty-eight female.Fifteen fale and sixteen female normal weight university students were enroled.Twelve weeks aerobics proceeded in Aerobic exercise group.Twelve weeks aerobics+resisitance exercise proceeded in aerobic exercise+resistance exercise group.Serum RBP4,body mass,body fat percent,body mass index,insulin resistance index,blood lipide,waist circumference,waist-hip ratio and VO2max detected before and after exercise. Recombination RBP4 injected rats to gain insulin resistance rats model. Insulin resistance rats divided into exercise group and quiet group,normal rats enroled as control group.Serum RBP4,insulin resistance index were detected after three weeks swimming.Expression and phosphorylation of insulin receptor and insulin receptor substance1 in skeletal muscle,adipose tissue and liver.Expression of phosphoric acid inositol-3-kinase, protein tyrosine phosphatase 1B and glucose intake were detected in skeletal muscle.Expression of glucokinase, phosphoenolpyruvate carboxykinase and hepatic glycogen were detected in liver.Results (1) There was positive relationshop between serum RBP4 and body mass,body mass index,body fat percent,insulin resistance index,waistcircumference,insulin,blood glucose,cholesterol,low density lipoprotein cholesterol after exercise intervene.There was negative relationship between serum RBP4 and high density lipoprotein cholesterol. (2)Twelve weeks aerobic exercise could notably decrease serum RBP4,body mass,body mass index,body fat percent,insulin, cholesterol,triglyceride,low density lipoprotein cholesterol,insulin resistance index,waist circumference,waist-hip ratio,and could notably increase V02max and high density lipoprotein cholesterol in overweight/obesity yonug university students.Twelve weeks aerobic exercise+resistance exercise could notably decrease serum RBP4,body mass,body massindex,body fat percent,insulin,blood glucose,cholesterol,triglyceride,low density lipoprotein cholesterol, insulin resistance index,waist circumference,waist-hip ratio,and could notably increase V02max and high density lipoprotein cholesterol in overweight/obesity yonug university students. (3) There was not significant difference in RPB4,body mass,body mass index,body fat percent,insulin, insulin resistance index,cholestrol,triglyceride,VO2max between aerobic exercise group and aerobic+resistance exercise group.There was significant difference in waist circumference,waist-hip ratio,blood gucose,high density lipoprotein cholestrol and low density lipoprotein choletrol between aerobic exercise group and aerobic+resistance exercise group. (4) There was positive relationship between RBP4 and body mass,body mass index,waist circumference,insulin, insulin resistance index after aerobic exercise in overweight/obesity. There was negtive relationship between RBP4 and V02max after aerobic exercise in overweight/obesity.There was positive relationship between RBP4 and body mass,body mass index,waist circumference,insulin, insulin resistance index after aerobic+resistance exercise in overweight/obesity. There was negtive relationship between RBP4 and V02max after aerobic+resistance exercise in overweight /obesity.There was positive relationship between decreasing RBP4 and decreasing body mass after aerobic+resistance exercise in overweight/obesity. There was positive relationship betweenΔRBP4 andΔbody mass,ΔBM,Δbody fat percentd,Δinsulin andΔinnsulin resistance index after aerobic+resistance exercise intervene. (5) Serum RBP4 and insulin resistance index increased more in exercise group rats and quiet group rats than control group rats.Serum RBP4 and insulin resistance index decreased more in exercise group rats than quiet group rats.Serum RBP4 and HOMA-IR increasd more in RBP4 intraperitoneal injection exercise groups than nomal control group, respetively.Glucose intake ratio in muscle cells and hepatic cells stimulating with or without insulin decresed more in exercise group rats and quiet group rats than control group.Glucose intake ratio in muscle cells and hepatic cells stimulating with or without insulin increase more in exercise group rats than quiet group rats. (6) Expression and phosphorylation of skeletal cells insulin receptor and insulin receptor substrate 1 decreased more in exercise group rats and quiet group rats than control group rats.There was not difference in expression and phosphorylation of skeletal cells insulin receptor between exercise group rats and quiet group rats.Phosphorylation of skeletal muscle insulin receptor 1 increased more in exercise group rats than quiet rats. (7) Expression of Phosphoric acid inositol-3-kinase and glucose intake ratio decreased more in exercise group rats and quiet group rats’ skeletal cells than control group rats.Expression of Phosphoric acid inositol-3-kinase and glucose intake ratio increased more in exercise group rats’ skeletal cells than quiet group rats. (8) Expression of protein tyrosine phosphatase IB increased more in exercise group rats and quiet group rats’ skeletal cells than control group rats.Glucose intake ratio decreased more in exercise group rats and quiet group rats’ skeletal cells than control group rats.Expression of protein tyrosine phosphatase 1B decreased more in exercise group rats’ skeletal cells than quiet group rats.Glucose intake ratio increased more in exercise group rats’ skeletal cells than quiet group rats. (9) Expression and activity of glucokinase decreased more in exercise group rats and quiet group rats’ liver than control group rats.Expression and activity of glucokinase increased more in exercise group rats’ liver than quiet group rats.Expression and activity of phosphoenolpyruvate carboxykinase increased more in exercise group rats and quiet group rats’ liver than control group rats.Expression and activity of phosphoenolpyruvate carboxykinase decreased more in exercise group rats’ liver than quiet group rats.Hepatic glycogen decreased more in exercise group rats and quiet group rats’ liver than control group rats.Hepatic glycogen increased more in exercise group rats’ liver than quiet group rats.Conclusion (1) Aerobic exercise and aerobic+resistance exercise could notably improve insulin resistance by decreasing serum RBP4,body mass, body mass index,bodyfat percent,insulin,bloodglucose,cholesterol,triglyceride, innsulin resistance index,waist circumference,waist-hip ratio of overweight/obesity youth.Aerobic exercise and aerobic+resistance exercise could notably improve insulin resistance by increasing V02max and high density lipoprotein cholesterol of overweight/obesity youth.There was not difference in serum RBP4 between aerobic exercise group and aerobic+resistance exercise group after exercise intervene. (2) There was positive relationship betweenΔRBP4 andΔbody mass,ΔBM,Δbody fat percentd,Δinsulin andΔinnsulin resistance index after aerobic+resistance exercise intervene. (3) RBP4 could result in insulin resistance by notable suppression the expression and phosphorylation of insulin receptor,insulin receptor substrate 1,Phosphoric acid inositol-3-kinase and glucose intake ratio in skeletal cells and hepatic cells.Three weeks swimming could improve insulin resistance through removing the suppression in insulin receptor substrate 1 phosphorylation and Phosphoric acid inositol-3-kinase expression of skeletal cell by RBP4 and through increasing skeletal cell glucose intake ratio. (4) RBP4 could result in insulin resistance by notable increase the expression of protein tyrosine phosphatase 1B and decrease glucose intake ratio in skeletal cell.Three weeks swimming could improve insulin resistance through removing the increase in protein tyrosine phosphatase 1B expression of skeletal cell by RBP4 and through increasing skeletal cell glucose intake. (5) RBP4 could notably restrain expression and activity of glucokinase in liver,increase expression and activity of phosphoenolpyruvate carboxykinase,decrease glycogen synthetize and increase glycogen heteroplasia.Three weeks swimming could increase expression and activity suppressed of glucokinase by RBP4,decrease expression and activity of phosphoenolpyruvate carboxykinase increased by RBP4,increase glycogen synthetize and decrease glycogen heteroplasia.
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