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Calla (Calla Lily) Araceae (Araceae), genus Calla (Zantedeschia) perennial plant whose flower-shaped unique , colorful and long flowering period, is good for decorative potted flowers , but also cut flowers, bouquets, baskets ideal material. But our calla breeding research lag , affecting the effectiveness of calla lily flower industry and prospects . Therefore, to calla breeding technology research, has a very important significance. In this thesis, a white calla lily (Zantedeschia aethiopica) as experimental material for anther culture, got a small spore origins regenerated plants and plant regeneration further biological traits were investigated to study the anther culture technique in breeding white calla application prospects. Test results showed that: ( 1 ) small white calla lily anther culture spore developmental pathway mainly: single-core or dual-core early late microspore after several equally split , develop into callus callus bud develops into full plants ; ( 2 ) white calla lily microspore callus induction medium for optimum : B5 basic medium , 8% sucrose , hormones KT 1.0 mg / L and 2,4-D 0.5 mg / L, agar 0.7 %, pH5.8; (3) white calla lily anther culture the most appropriate way to deal with stress : 4 ℃, 6 days; ( 4 ) donor plants in different growing seasons have a certain influence on the anther culture , in which the growth of winter donor plants provided anther callus induction rate ; ( 5 ) \analysis showed that anther culture regenerated plants originated in the small spores ; ( 7 ) about 18 % of small spore Origins of diploid plants in floral traits such as length and petiole length appeared on the transgressive phenomenon, there are 34.8% of the strains produce seeds ; ( 8 ) plant propagation test results show : best explant was growing point ; ideal medium for : MS basic medium , 3% sucrose , hormones as KT 1.0mg / L, IBA0.05 mg / L, 0.7% agar , pH value of 5.8 , budding rate of up to 80%.
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