|
The fungus is the most important one of the mushroom cultivation in China, Heilongjiang Province cultivars, the cultivated fungus enriching industry has become a pillar of a number of cities and counties. The hyphae aging is often faced with the problem of the fungus production and seed production process, to the the fungus production of a great deal of economic loss. Domestic and foreign fungus mycelium aging research is still blank control mycelium aging lack the necessary theoretical basis. Based on this, the study of the aging of the fungus mycelium preliminary exploration to reveal the fungus hyphae aging mechanism to provide scientific data. Micro-morphological study of the aging process by fungus mycelium 30d after inoculation of the fungus mycelium, light microscopy mycelium uniform compact structure, cell wall smooth; electron microscopy to the structural integrity of cells rich inclusions, each kinds of structured organelle morphology, no aging. 60d after inoculation, the light microscope mycelium part of the swelling, deeper color; electron microscope, cell wall loose, mitochondria and vacuole enlargement nuclei irregular swelling, nucleoli disappear lipid droplets and vesicles increased, and there is a small amount of high electron-dense The osmiophilic black particulate matter, that the hyphae begin to age. 90d, the light microscope part mycelium severe swelling and deeper color; electron microscope, mitochondria and vacuole swelling, rupture of some nuclei, lipid droplets, vesicles and electron-dense osmiophilic high black particulate matter increased significantly, cell wall more loose. 120d, light microscope, many hyphae begin to break further deepen the color; electron microscope, cell wall collapse, followed the disintegration of the membrane system, the partial dissolution of mitochondria and other organelles disappear. 150d, light microscope, most of the mycelium completely broken and lost mycelial morphology; electron microscope, the cell membrane and its contents disappeared, leaving only part of a serious collapse of the cell wall wreckage. It is suggested that the aging of the fungus mycelium is an individual irreversible transition gradually to the whole process. Through research on the the mitochondrial morphology fungus mycelium aging process and the number of changes that, with the growth of the fungus mycelium strain age, mitochondrial swelling gradually deformed, and finally even rupture, and the number will continue to reduce. Description the fungus mycelium of aging and mitochondrial morphological changes and the number has a close relationship. Physiological changes of the aging process of the fungus mycelium was positively correlated with changes of malondialdehyde (MDA), with the aging of the fungus mycelium, a certain regularity, can be used as an indicator of aging fungus mycelium; superoxo dismutase (SOD) activity with increased strain age of the fungus mycelium downward trend, but the 30d to 90d decline 90d to 150d decreased less regularity; peroxidase (POD) activity in the the fungus mycelium aging process is low, there is no obvious pattern; catalase (CAT) activity increased with the age of the fungus mycelium fungus growth overall downward trend, but the ups and downs, the regularity is not significant. Add KH 2 PO 4 affects neither the mycelium normal growth, can play four different inorganic salts fungus mycelium aging studies have shown that by adding good anti-aging effect, when 90d after inoculation, the mycelium is still white and thick, and to add a concentration of 0.3% KH 2 PO 4 combined effect of the most significant. Add the MgSO 4 mycelium aging and long speed have a certain extent, but the anti-aging effect is not significant; addition of NaCl anti-aging effect is significant, but also severely inhibited growth of the mycelium not conducive to production; Add CaCO 3 Notwithstanding the promotion of the role of mycelial growth, but also promote the aging process of the mycelium and the mycelium growing sparse, poor ability to regenerate.
|