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Ginseng and their descendants total DNA into Potato physiological and biochemical characteristics of

Author: XuNing
Tutor: HongYaHui
School: Hunan Agricultural University
Course: Cell Biology
Keywords: immersion training method RAPD physiological and biochemical Detection Total carbohydrate amino acid protein saponins
CLC: S532
Type: Master's thesis
Year: 2011
Downloads: 22
Quote: 0
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Abstract


Ginseng total DNA is imported into potato callus small cell groups using immersion and training method. and obtained transgenic plants. Using Ginseng DNA, transformed potato plantlets’DNA, Untransformed Potato’DNA As a template for PCR amplification. The results showed that transformation of potato and ginseng are the same size of DNA fragments. and untransformed Potato not to exist that. To obtain transformed potato have difference genetic characteristics.By physiological and biochemical Detection, show that the Total carbohydrate content of transformed potato tubers was 1.183%,and the Total carbohydrate content of untransformed potato tubers was 2.060%. The Total carbohydrate content of transformed potato lower than the Total carbohydrate content of untransformed potatoThe accumulation of photosynthetic products is increase in transformed potato. 17 amino acids in transformed potato were significantly higher than untransformed potato. aspartic acid increased 79%, threonine 42%,31% serine, glutamate increased by 46%,45% glycine, alanine increased by 30%,19% cysteine, valine Increase of 23.4%, methionine increased 153%,42% isoleucine, leucine 42%,9% increase in tyrosine and phenylalanine increased 57%,88% lysine, group Acid increased by 22%, 46% increase in arginine, proline 25%. Moreover, the matter content of saponins in potato increased by 47%.Finally, the protein content in transformed potato tubers is 1.9mg/500mg.and protein content of untransformed potato tubers is 1.4mg/500mg. Protein content of potato tubers transformed than untransformed potato tuber protein content increased. Content is higher than in untransformed potato tuber.

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CLC: > Agricultural Sciences > Crop > Tuber Crops > Potatoes ( potatoes )
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