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Proteomic Analysis of Leaf Intercellular Fluid from Stripe Rust Resistance Wheat

Author: HuXinMing
Tutor: YangYouCai;PanYingHong;XuShiChang
School: Hunan Agricultural University
Course: Ecology
Keywords: Wheat Stripe rust Molecular Ecology Proteome Two-dimensional electrophoresis Intercellular eluent
CLC: S512.1
Type: Master's thesis
Year: 2011
Downloads: 42
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As China's major food crops of wheat, more than 3.5 million mu of cultivated area, production accounts for about 20% of the country's total grain output, while wheat stripe rust (Puccinia striiformis f.sp.tritici) has been a major disease of wheat, a serious threat to China's wheat production and food security. Therefore, the study of wheat stripe rust and the interaction between the host wheat germ reveal interactions between mechanisms in wheat cultivation and use of resistant varieties and stripe rust prevention has an important theoretical and practical significance. In this paper, stripe rust resistance gene Yr5 containing wheat near-isogenic lines as the material, a comparative analysis of wheat leaf intercellular fluid proteins in group and total leaf proteome, as well as after the infection by stripe rust CYR32 leaf cells near-isogenic lines interstitial fluid proteome discussed intercellular fluid on stripe rust spore germination, the main results are as follows: (a) establish and improve the wheat leaf intercellular fluid protein separation and analysis system, a comparative analysis of wheat leaf intercellular fluid proteins differences in total leaf protein. Two-dimensional electrophoresis analysis showed resistance to stripe rust of wheat near-isogenic lines Taichung29 * 6/yr5 leaf intercellular glue protein sample can be detected in the figure 1042 ± 47 个 total leaf protein samples with different protein spots. Take 100 points arbitrary mass spectrometry analysis, identification number has been confirmed to exist in wheat leaf intercellular fluid proteins, such as glucanase glucanase, chitinase, peroxidase. (2) resistance to stripe rust near isogenic lines Taichung29 * 6/Yr5 and stripe rust sensitive recurrent parent Taichung29 vaccination stripe rust (CYR32) 36 hours after the leaves intercellular fluid proteome appeared obvious changes. Vaccination stripe rust and analog vaccination Taichung29 * 6/Yr5 appeared 23 highly significant differences in protein spots, and appeared 31 Taichung29 very significant differences in protein spots between the two very significant point of difference from five identical . The Taichung29 and stripe rust inoculation appeared 17 Taichung29 very significant differences in protein spots appeared 13 analog inoculation significantly different protein spots, these very significant point of difference and analog stripe rust inoculation inoculation no different. Preliminary analysis of the expression of these proteins may be infected with stripe rust resistance to stripe rust of wheat and wheat-related, but did not get a higher MALDI-TOF/TOF mass spectrometry protein identification rate. (3) Preliminary analysis shows, Taichung29 * 6/Yr5 and taichung29 intercellular fluid extract of stripe rust spore germination have a more significant impact, when the wheat leaf intercellular fluid extract diluted from the original concentration of 0.2mg/ml 0.02mg/ml, the stripe rust spore germination restored to near normal state. And intercellular fluid extract on the growth of germ tubes after germination also have a certain impact.

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CLC: > Agricultural Sciences > Crop > Cereal crops > Wheat > Wheat
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