Dissertation > Excellent graduate degree dissertation topics show

Proteomic Analysis of Leaf Intercellular Fluid from Stripe Rust Resistance Wheat

Author: HuXinMing
Tutor: YangYouCai;PanYingHong;XuShiChang
School: Hunan Agricultural University
Course: Ecology
Keywords: Wheat Stripe rust Molecular Ecology Proteome Two-dimensional electrophoresis Intercellular eluent
CLC: S512.1
Type: Master's thesis
Year: 2011
Downloads: 42
Quote: 0
Read: Download Dissertation

Abstract


As China's major food crops of wheat, more than 3.5 million mu of cultivated area, production accounts for about 20% of the country's total grain output, while wheat stripe rust (Puccinia striiformis f.sp.tritici) has been a major disease of wheat, a serious threat to China's wheat production and food security. Therefore, the study of wheat stripe rust and the interaction between the host wheat germ reveal interactions between mechanisms in wheat cultivation and use of resistant varieties and stripe rust prevention has an important theoretical and practical significance. In this paper, stripe rust resistance gene Yr5 containing wheat near-isogenic lines as the material, a comparative analysis of wheat leaf intercellular fluid proteins in group and total leaf proteome, as well as after the infection by stripe rust CYR32 leaf cells near-isogenic lines interstitial fluid proteome discussed intercellular fluid on stripe rust spore germination, the main results are as follows: (a) establish and improve the wheat leaf intercellular fluid protein separation and analysis system, a comparative analysis of wheat leaf intercellular fluid proteins differences in total leaf protein. Two-dimensional electrophoresis analysis showed resistance to stripe rust of wheat near-isogenic lines Taichung29 * 6/yr5 leaf intercellular glue protein sample can be detected in the figure 1042 ± 47 个 total leaf protein samples with different protein spots. Take 100 points arbitrary mass spectrometry analysis, identification number has been confirmed to exist in wheat leaf intercellular fluid proteins, such as glucanase glucanase, chitinase, peroxidase. (2) resistance to stripe rust near isogenic lines Taichung29 * 6/Yr5 and stripe rust sensitive recurrent parent Taichung29 vaccination stripe rust (CYR32) 36 hours after the leaves intercellular fluid proteome appeared obvious changes. Vaccination stripe rust and analog vaccination Taichung29 * 6/Yr5 appeared 23 highly significant differences in protein spots, and appeared 31 Taichung29 very significant differences in protein spots between the two very significant point of difference from five identical . The Taichung29 and stripe rust inoculation appeared 17 Taichung29 very significant differences in protein spots appeared 13 analog inoculation significantly different protein spots, these very significant point of difference and analog stripe rust inoculation inoculation no different. Preliminary analysis of the expression of these proteins may be infected with stripe rust resistance to stripe rust of wheat and wheat-related, but did not get a higher MALDI-TOF/TOF mass spectrometry protein identification rate. (3) Preliminary analysis shows, Taichung29 * 6/Yr5 and taichung29 intercellular fluid extract of stripe rust spore germination have a more significant impact, when the wheat leaf intercellular fluid extract diluted from the original concentration of 0.2mg/ml 0.02mg/ml, the stripe rust spore germination restored to near normal state. And intercellular fluid extract on the growth of germ tubes after germination also have a certain impact.

Related Dissertations

  1. Functional Analysis of Proteins Encoded by RNA2 of Wheat Yellow Mosaic Virus,S435.121
  2. Pathogenic Differentiation and Genetic Diversity of Wheat Sharp Eyespot in Henan Province,S435.121
  3. Cellular and Proteomic Analyses of Endocytosis Mechanism Involved in Pollen-tube Growth in Nicotiana Tabacum,Q942
  4. The Identificationand Rapdanalysis of Wheat Root Lesion Pathogens in Henan Province,S435.121
  5. Nitrogen Regulation and Management on High-yield Wheat-maize Rotation System,S512.1
  6. The Difference of Proteome Expression of Low Light Stress and Regular Light Recovery on Growth of Maize Seedling,S513
  7. Effect of Nitrogenapplication Patterns on Variation of Soil Nitrate-N and Grain Yield of Wheat and Maize,S512.11
  8. Study on Nitrogen Accumulation Characteristics of Winter Wheat with Yield of 10t/ha, Diagnosis and Nitrogen Application Technologies,S512.1
  9. Proteomic Analysis of Vibrio Alginolyticus in Response to Four Types of Antibiotics,S941
  10. Optimization of Fermentation Conditions of Recombinant Lipoxygenase for the Production in Wheat Flour,TS201.25
  11. Study on Extraction,purification and Antioxidant Activity of Wheat Germ Polysaccharides,TS210.1
  12. Determination of Soil Enzyme Activity and Extraction, Purification and MS Identification of Soil Microbial Total Protein,S154
  13. Characterization of Mineral Phosphate-Solubilizing Bacterium K3 and Colonization under Different Soil,S144.9
  14. Design and Implementation of the Growing Visualization System for Wheat Population,S512.1
  15. Trajectory Analysis of Wheat Aphids During Long-distance Migration Using HYSPLIT Model,S433
  16. Genetic Structure of the Wheat Powdery Mildew Populations from Henan, Hubei and Chongqing Province (City),S435.121
  17. Cloning 1Dx5 Gene from the Xinjiang Wheat Variety and Constructing Its Expression Vector,S512.1
  18. Studies on Regulating Effect of Nitrogen Rates on Starch Granule Distribution in Grain of Wheat,S512.1
  19. Accumulation of High Molecular Weight Glutenin Subunits in Wheat Grain as Regulated by Nitrogen Rate,S512.1
  20. Effects of Pre-Anthesis Drought Hardening on Grain Yield and Quality Formation in Wheat under Post-Anthesis Drought and Its Physiological Mechanism,S512.1
  21. Difference in Development between Culm and Tiller Roots and Their Contribution to Grain Yield and Quality in Wheat (Triticum Aestivum L.),S512.1

CLC: > Agricultural Sciences > Crop > Cereal crops > Wheat > Wheat
© 2012 www.DissertationTopic.Net  Mobile