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Effects of Propofol and Etomidate on Retina Induced by Intestinal Ischemia/Reperfusion in Rats

Author: ZhaoJuan
Tutor: GuEnHua
School: Tianjin Medical University
Course: Anesthesiology
Keywords: Ischemia-reperfusion Retina Propofol Etomidate Rats Oxygen radicals
CLC: R965
Type: Master's thesis
Year: 2011
Downloads: 17
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Abstract

Objective: by clamping the superior mesenteric artery (superior mesenteric artery, SMA) to establish intestinal ischemia-reperfusion (ischemia reperfusion, I / R) model, to observe whether retinal damage can lead to intestinal ischemia-reperfusion, and oxygen free radical damage commonly used intravenous anesthetics propofol and etomidate retinal damage and other related indicators of change, and to explore the experimental research data to reveal its mechanism of action, and to provide the basis of the theoretical basis for its clinical application. Methods: 32 healthy clean male Wistar rats were randomly divided into four groups, namely the sham group (S), intestinal ischemia-reperfusion group (I group), propofol (P group) and etomidate ester group (E). Each group are heparin (2mg/kg) (?) (?) Systemic anticoagulation. S group separation SMA, inferior vena cava syringe pump continuous infusion of 0.9% saline 10ml / (kg · h). I the group clipping of SMA 1h, reperfusion 2h, and the inferior vena cava syringe pump continuous infusion of 0.9% saline 10ml / (kg · h). The P group E clipping SMA 1h, inferior vena cava syringe pump continuous infusion of 0.9% saline 10ml / (kg · h) reperfusion 2h 5min before reperfusion replace the saline respectively Yung propofol 10mg / (kg · h) and etomidate 0.2mg / (kg · h). 2h reperfusion, the rats were killed, the removal of the eye ball, take the right eye and from the small intestine of the ileocecal 20cm some sealed in a 10% formalin solution until the hematoxylin - eosin (with hematoxylin eosine HE) staining light microscope observed histological changes. Left eye treated, stored in a liquid nitrogen tank, tissue homogenates was measured to be in the future production of superoxide dismutase (superoxide dismutase, SOD) and malondialdehyde (malondialdehyde, MDA). The data applications SPSS 17.0 statistical analysis. Results: 1. Intestinal tissue pathological changes: S group of small intestinal mucosa basically normal arrangement rules; the I group small intestine villi subcutaneous gap expansion associated with mucosal epithelium and lamina propria moderate separation, and the lamina propria of the increased number of neutrophils, some fluff top breakage; subcutaneous gap mild expansion in the small intestine of the P group E, the mucosal epithelium and lamina propria mild separation, an increase in the lamina propria neutrophils mild, damaged part of the villus tip. 2. Retinal tissue pathological changes: S group retinal structure level distinct outer nuclear layer of tightly packed, inside and outside the section neat rules and clear boundaries. Many different sizes of gap between the I group the inner retinal edema, the inner layer and the nerve fiber layer, ganglion cells dispersed due to the accumulation of edema fluid; outer nuclear layer decomposition heavier destruction; P group and E group retinal edema mitigate, the small blood vessels in the open state, it had not previously been open to compensatory function, cell arrangement than the rule. I group retinal tissue SOD activity decreased (57.50 ± 10.88) compared with the S group, MDA content increased (11.60 ± 2.47) (p lt; 0.05); comparison group I, the P group E retinal tissue SOD activity elevated (94.50 ± 29.13,96.25 ± 29.61), the content of MDA lower (5.81 ± 2.92,7.08 ± 1.39) (p lt; 0.05); the P group Group E and the difference was statistically significance (p gt; 0.05) . Conclusion: 1. Successfully copied the rat intestinal ischemia-reperfusion model, and intestinal ischemia the retinal tissue morphology reperfusion significantly damaged and caused the decline in antioxidant capacity, oxygen free radicals increased lipid peroxidation corresponding indicators changed significantly. 2.10mg / (kg · h) propofol and 0.2mg / (kg · h) etomidate significantly reduced ischemia-reperfusion-induced retinal damage, which may be related to enhanced antioxidant capacity, reducing oxygen free radicals generation, reducing lipid peroxidation injury and brain protective effect is related to, but was no significant difference between.

Effects of Propofol and Etomidate on Retina Induced by Intestinal Ischemia/Reperfusion in Rats

Abstract

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