Dissertation > Excellent graduate degree dissertation topics show

The Study of Adventitia Applied Slow-releasing Rapamycin Inhibiting Intima Hyperplasia of Vascular Grafts in Rabbits

Author: BiMingXia
Tutor: ZuoShuGuang
School: Shandong University
Course: Internal Medicine
Keywords: Vascular graft Intimal hyperplasia Rapamycin p27kip1
CLC: R965
Type: Master's thesis
Year: 2011
Downloads: 14
Quote: 0
Read: Download Dissertation

Abstract

Objective: To establish rabbit infrarenal aortic vascular graft model given rapamycin after vascular adventitia, and α-actin, vWf, PCNA and study the transplant vascular pathology morphological change and p27kip1 in the transplanted blood vessels in the expression and blood vessels proliferation of smooth muscle cells (VSMC) relationships explore rapamycin inhibits the transplant vascular restenosis mechanism of action, and by the vascular adventitia administration feasibility. Methods: 36 healthy male New Zealand white rabbits weighing 2.5 to 3.0 kg, were randomly divided into A, B, C, D, E, F6 groups of six each were constructed inactivated allogeneic abdominal aortic transplantation model and PTFE artificial vascular abdominal aortic transplantation model groups were given different treatment, vascular anastomosis is completed. Group A: inactivated allogeneic abdominal aortic transplantation group graft vessel are not given any treatment; B group: inactivated allogeneic abdominal aorta transplantation in the transplant vascular adventitia and anastomotic around smear preparation of a good 20% pluronic F-127 gel 0.5 ml of poly; C group: inactivated allogeneic abdominal aortic transplantation, transplant vascular adventitia and around the anastomotic smear carrying rapamycin 0.5mg pluronic F-127 poly gel 0.5 ml; D group: PTFE artificial blood vessels and abdominal aorta transplantation group graft vessel not given any treatment; Group E: PTFE artificial blood vessels and abdominal aorta transplant, transplant vascular adventitia and around the anastomotic smear preparation of a good 20% pluronic F- the 127 poly gel 0.5 ml of; F group: PTFE artificial blood vessels and abdominal aorta transplant, transplant vascular adventitia and around the anastomotic smear carry rapamycin 0.5mg pluronic F-127 poly gel 0.5ml. 4 weeks after transplantation Get vascular tissue morphology observed transplant intimal hyperplasia, computer image analysis system for measuring the the transplantation intimal medial thickness and calculate the degree of intimal hyperplasia (intima / media), immunohistochemistry The SP method detection the transplanted vascular alpha-actin, vWf, PCNA and p27kipl expression. SPSS13.0 statistical software for statistical analysis of the experimental data, the experimental data are presented as mean ± standard deviation (x ± s) said. Results: 1. Successfully build inactivated allogeneic abdominal aortic transplantation model and of PTFE artificial blood vessels and abdominal aorta transplantation model. 2 January after surgery, tissue morphology observation and computer image analysis found that the A group and B group compared with the C group intimal hyperplasia obvious D group, E group than in the group F endometrial hyperplasia, the difference was significant (P lt ; 0.05). Elastic fiber stain (Weigert's resorcin fuchsin staining): the go activity transplant vascular surgery four weeks still visible the complete elastic fiber staining, transplant vascular elastic fibers and other layers of the structure completely preserved. 4α-actin immunohistochemistry observations: intimal hyperplasia visible expression of α-actin-positive, similar to the vascular smooth muscle α-actin positive expression, expression of smooth muscle activity in the most superficial cells layer confirmed intimal hyperplasia. 5.vWf immunohistochemical observation: after transplantation varying degrees of intimal hyperplasia, the most superficial cells arranged in a monolayer, visible vWf staining confirmed the proliferation of endometrial outermost layer of the vascular endothelial cell monolayer arranged. 6. PCNA of p27kip1 immunohistochemical observations: are nuclear staining, positive cells showed brown. Groups transplant vascular proliferation of endometrial cells showed positive expression. The 7.α-actin and PCNA expression of group C and Group F, respectively, compared with A, group B and D, E group significantly reduced, the difference was significant (P lt; 0.05). p27kipl expression of group C and F, respectively, compared with A, group B and D, E group increased significantly, and the difference was significant (P lt; 0.05). A group and B group, each group of data between the D group and E group showed no significant differences in sex (P gt; 0.05). Conclusion: formaldehyde treatment to active allograft can effectively preserve the organizational structure of the blood vessels, and effective removal of vascular antigen. 2. Intimal hyperplasia is a major factor in causing vascular graft restenosis. The main component of the intimal hyperplasia expression of smooth muscle activity, suppress intimal hyperplasia that inhibit the excessive proliferation of smooth muscle is the most critical factor in maintaining vascular long-term patency. 3. Pluronic F-127 poly gel carry rapamycin smear transplantation the vascular adventitia effectively inhibit the transplant vascular smooth muscle cells proliferation. 4 rapamycin inhibits the transplant vascular restenosis mechanism its raised the the transplant vascular tissue p27kipl expression, inhibition of cell proliferation cycle related. 5 after transplant vascular adventitia administration is feasible and effective.

The Study of Adventitia Applied Slow-releasing Rapamycin Inhibiting Intima Hyperplasia of Vascular Grafts in Rabbits

Abstract

Related Dissertations