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Study of Sulfurmetabolism Function for luxS Gene of the Streptococcus Mutans

Author: RenZhiMing
Tutor: YuDanNi
School: Tianjin Medical University
Course: Clinical Stomatology
Keywords: Streptococcus mutans the luxS gene mutation strains Biofilm Sulfur metabolism Mean fluorescence intensity
CLC: R781.1
Type: Master's thesis
Year: 2011
Downloads: 19
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Abstract

Objective To establish a biological model of Streptococcus mutans standard strains and luxS gene mutant observe biofilm differences of the two strains in different growth period ; through in deformation of the two strains in different conditions medium growth and biofilm difference of the comparative analysis of research luxS gene Streptococcus the sulfur metabolic function . The method utilizes the Streptococcus mutans standard strains and early experimental build successful luxS gene mutant , circular slide carrier biofilm model , the two strains in different periods biofilm morphology inverted fluorescence microscope by acridine orange staining differences ; nutritional supplement experimental culture of two strains , the determination of the absorbance value (A) through different periods of growth of the two strains , growth differences ; using confocal laser scanning microscopy (CLSM) observed 24 h two strains biofilm thickness and mean fluorescence intensity was measured . Results inverted fluorescence microscopy direct dynamic observation of the well plate biofilm the various endoscopic view of two strains biofilm morphology ; 6h bacterial aggregation began film-forming , 18 ~ 24 h to form the best adhesion slides firmly difficult obliterated ; 48 h standard strain biofilms attached to glass slides ability than mutants weak , easily the overall slide ; biofilm specimens of the two strains treated for three months after the end of the microscope morphology change . Mutant certain concentration of cysteine ??, respectively, in the medium growth and biofilm growth , but at the end , and the level of standard strain ; were added to a certain concentration of methionine and SAH mutant growth and biofilm been supplement , but not standard strain level ; mutant growth and biofilm water when adding a certain concentration standard strains of supernatant were significantly increased and more than the standard strain level ; when the two strains were added to SAM growth and biofilm thickness there are different degrees of reduction . The biofilm thickness no correlation between the fluorescence intensity . Conclusion the deformation of Streptococcus standard strains with luxS gene mutant biofilms have different spatial structure , morphological diversity , and at different times , different biofilm morphology, the interaction between the bacteria and the adhesion ; luxS gene not only in Streptococcus mutans have the density sensing capabilities , and also plays a role in nitrate sulfur metabolism .

Study of Sulfurmetabolism Function for luxS Gene of the Streptococcus Mutans

Abstract

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