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Ubiquitin - proteasome pathway-mediated cell protein degradation is a complex and meticulous process, this highly selective protein degradation pathways play an important role in regulating the cell cycle, gene transcription and expression of the antigen was. But so far, the molecular mechanism and function of ubiquitin - proteasome pathway is not well understood. The 26S proteasome is composed by a plurality of subunits of the composite body, the composite body is composed of two parts, the part of the 20S degradation complex, and the other part is a 20S degradation complex (CP) at both ends of the 19S regulator compound (RP). 20S degradation complex is the center of hydrolysis of the substrate protein, composed by 7 alpha subunit and beta subunit, alpha 1-7 beta in the beta 1-7 < sub> 1-7 α 1-7 of the barrel-shaped structure. There are three in mammalian cells can be induced by interferon (IFNγ) in the beta subunit, β1i β2i β5i can replace beta1 beta2, beta5 formed the immune proteasome function mainly in antigen presentation. 19S regulator compound at least 17 subunits, contains two sub-structures, are \\recycling of ubiquitin; \protein into the 20S degradation complex, the hydrolysis of the substrate protein. The liver is the body's largest organ, the material metabolism, energy conversion and supply hub, plays an important role in the activities of human life. Construct protein network map of the human liver cells, the mechanism of liver disease and treatment has a major significance. By RT-PCR method from human liver tissue obtained by the full-length ORF of the 26S proteasome subunit, the ORF of these subunits were cloned into the yeast two-hybrid vector, into the inter-subunit yeast S.cerevisiaeAH109, interaction analysis. We received a total of 34 of the 26S proteasome subunit full-length ORF, detected 1,156 pairs of the 26S proteasome subunit interaction experiments, observed 112 positive results, including 18 pairs of interactions between the subunits is a two-way ; the same time, we use the method of the GST-pull down, verify that nearly 10% of the yeast two-hybrid assay results; also co-localized expression of different fluorescent labels subunit interaction transfected into HeLa cells, the concept of Chaya Ji. We verify the 26S proteasome subunit interaction between different systems, demonstrates our experimental results. 41% of the yeast two-hybrid positive results reported in the original literature, this is more proof of the correctness of our results. We also got some new 26S proteasome subunit interactions, which laid the foundation for understanding the function of the 26S proteasome.
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