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Pharmacodynamic Constituents of TCM research is the development of active ingredients specifically, efficacy, quality stable and controllable generation proprietary basis and is the key to achieving modernization of Chinese medicine. Material basis of traditional Chinese medicine there are many ways, the subject of the use of separate restructuring ideas beans bile pills material basis for research. According beans bile pills group prescription patchouli leaves and pig powder chemical composition, group side characteristics, reasonable use of modern industrial technology, patchouli leaves and pig powder obtained in a variety of chemical components of traditional pills as a reference, for anti- inflammation Allergy chemical composition analysis tracking, anti-inflammatory allergy pill proven beans gall sexual component of patchouli oil and hyodeoxycholic; patchouli oil, and for the group hyodeoxycholic party components for compatibility and dose optimization research for the development of new generation based on preliminary studies lay the new Chinese medicine. Beans bile pills patchouli leaves and pig powder by the two flavor components. Beans bile pills from the Qing Dynasty \fire caused by nasal congestion, runny nose, forehead, headache embolism, more than two hundred years of clinical history. Beans bile pills efficacy, but the product rough, obsolete production technology exists, dosage form behind, taking the volume and other deficiencies. The research project carried out as follows: 1, patchouli leaf beans bile pills effective part in anti-inflammatory and anti-allergic effect screening test screening effective part of patchouli leaf, and beans bile pills medicines for the control, the results show that the broad rugosa leaves contain volatile oil-soluble portion and a lipophilic portion, although anti-inflammatory effect, but significantly lower potency volatile oil site, patchouli leaves, beans bile pills medicine; patchouli leaves contain volatile oil-soluble parts and The site does not have a fat-soluble anti-allergic effects, while parts of volatile oil and patchouli leaves, beans bile pills with anti-allergic effects. Patchouli leaf volatile oil also has anti-inflammatory, anti-allergic effects, and its intensity close patchouli leaves, beans bile pills medicines, so you can determine the patchouli leaf beans bile pills active site is mainly Patchouli Essential Oil. Compare supercritical carbon dioxide extraction (less with SFE-CO 2 represent) and steam distillation method to extract patchouli essential oil from leaves. The results show that by steam distillation, only to have aromatic water, is extremely difficult to obtain volatile oil. Therefore, this project uses SFE-CO 2 Extraction of patchouli essential oil from leaves and determine its extraction process: patchouli leaves crushed into meal, SFE-CO 2 Extract extraction parameters extraction temperature 53 ℃, extraction pressure 13MPa, analytic pressure 5.5MPa, analytical temperature 63 ℃, carbon dioxide (CO 2 ) flow 400L / h, extraction time was 2.0 hours, too Guanghuo Geranium Essential Oil. Using SFE-CO 2 patchouli leaf extract obtained volatile oil, patchouli oil compared with commercially available, both consistent quality, are in line with the 2005 edition of Chinese Pharmacopoeia requirements. 2, patchouli oil on the beans bile pills pharmacodynamics beans with patchouli oil to replace the bile pills samples obtained patchouli leaf herbs called refined beans gall party to the refining side of the composition of bile beans patchouli oil and pig powder. Compare refined beans gall square, beans bile pills, patchouli oil, pig powder pharmacodynamic results show that: (1) refined beans gall square, patchouli oil, pig bile powder and beans all have anti-inflammatory pills. Acetic acid induced inflammatory indicators peritoneal capillary permeability, carrageenan-induced rat paw edema, xylene-induced ear edema, rat granuloma and other four kinds of acute and sub-acute inflammation model. Refined beans rat bile party carrageenan induced paw edema, xylene-induced ear edema model of acute inflammation, granuloma Asia rat model of acute inflammation inhibition strength and beans bile pills rather, on the inhibition of capillary permeability induced by acetic acid is superior to beans bile pills. Patchouli oil, pig powder compatibility of synergies between the two obvious. Tip replace beans with patchouli oil patchouli leaf bile pills, can keep beans gall party anti-inflammatory effect. (2) Refined beans gall square, patchouli oil, pig powder and beans bile pills original parties have anti-allergic effects. Allergy indicators OVA sensitized guinea pig ileum muscle contraction allergic reactions, the same kind of rat passive cutaneous anaphylaxis (PCA), 2,4 - Dinitrochlorobenzene induced ear skin delayed-type super- allergic reactions such as anaphylaxis model. Refined beans gall side of the OVA sensitized guinea pig ileum muscle contraction allergic reactions, the same kind of rat passive cutaneous anaphylaxis (PCA) inhibition than beans bile pills; 2,4 - Dinitrochlorobenzene induced ear inhibition of delayed-type hypersensitivity skin strength equivalent hopane bile pills. Patchouli oil, pig powder compatibility of synergies between the two obvious. Tip replace beans with patchouli oil patchouli leaf bile pills, can keep beans gall party anti-allergic effects. (3) refined beans gall side has in vivo antibacterial effect. Refined beans gall square test for the in vitro antibacterial test bacteria (Staphylococcus aureus, hemolytic streptococcus, beta-hemolytic streptococcus, Streptococcus pneumoniae, catarrhal bacteria, Corynebacterium diphtheriae, Escherichia coli, Pseudomonas aeruginosa and Candida albicans) have different levels of antibacterial activity; against pneumococcal infection, beta-hemolytic streptococcus, staphylococcus aureus in mice, all have a significant role in reducing mortality. Tip replace beans with patchouli oil patchouli leaf bile pills, can keep beans gall parties in vivo antibacterial activity. (4) refined beans gall square, patchouli oil, pig bile powder and pills are beans with enhanced immune function. Enhance immune function indicators mouse reticuloendothelial system phagocytosis carbon clearance model. Refined beans gall square, patchouli leaf volatile oil, pig powder were right mouse reticuloendothelial system phagocytosis of carbon clearance has significantly enhanced role; refined beans gall Fangzeng Jiang immunity strength and beans bile pills considerably. Tip replace beans with patchouli oil patchouli leaf bile pills, can remain immune enhancement beans gall side effects. The results show that the composition of patchouli oil and refined pig pink beans gall side has anti-inflammatory, anti-allergic, anti-bacterial, and enhance immune function; patchouli oil and pig powder compatibility between applications, anti-inflammatory, antiallergic efficiency in significant synergies; anti-inflammatory, anti-allergic, anti-bacterial, and enhance the efficacy of the immune indicators, refined beans equivalent to or even better than beans gall bladder bile party pills. The results suggest that bile pills beans patchouli leaves with patchouli oil alternative, you can keep the original beans bile pills efficacy. 3, pig powder chemical composition of the liver and blood biochemical indexes by the patchouli oil and refined pig powder consisting of beans gall prescription, medicine is still the pig powder directly, resulting in difficulty forming, unpleasant odor, health Learn sad Guan, quality is difficult to control, and because the pig powder containing a certain amount of cholesterol, long-term use may cause high blood cholesterol and other defects, the urgent need to find out the use of modern science and technology and their efficacy equivalent active ingredient effective parts or replace . Hyodeoxycholic pig powder is the main active ingredient, with a chemical composition of a single, stable quality control, and no cholesterol, less impurities, easy molding and other advantages. Modern pharmacological studies have found that cholic acid is a pig powder pharmacological effects of the active ingredient in many aspects, especially in the anti-inflammatory, anti-allergic, anti-bacterial and enhance immunity, cholic acid more prominent role. The pig powder of cholic acid containing mainly hyodeoxycholic for hyodeoxycholic substitute beans bile pills in the pig powder provides a theoretical basis. With patchouli oil and beans bile composition hyodeoxycholic valid prescription, namely beans gall valid prescription composed of patchouli oil and hyodeoxycholic. Compare refined beans and beans gall bladder party valid prescription for blood cholesterol and liver dysfunction effects. The results showed that 90 days after the drug, refined beans blood cholesterol gall party, ALP, AST levels were higher than the control group; refined beans gall square ALT levels with the control group fairly; withdrawal 14 days, refined beans gall square blood cholesterol, ALP, AST, ALT levels were normal; beans gall 90 days after the effective prescription drug withdrawal 14 days of blood and blood cholesterol, ALP, AST, ALT levels were considerably compared with the control group. These results suggest that refined beans gall parties will lead to long-term medication blood cholesterol and some aminotransferase, beans gall valid prescription can avoid this result. Hyodeoxycholic alternative pig powder applied to beans bile pills, avoids blood cholesterol and liver dysfunction influence to get better security protection. 4, hyodeoxycholic right beans bile pills pharmacodynamics beans gall valid prescription to avoid cholesterol in the blood and liver dysfunction influence whether it can maintain the efficacy of beans bile pills still need experimental evidence. Anti-inflammatory, anti-allergic, antibacterial and immune function tests and other indicators to assess hyodeoxycholic alternative is feasible. The results showed that: (1) beans gall effective component parties hyodeoxycholic, patchouli oil, refined beans gall party pig bile powder and beans were the original party pill induced by acetic acid increased capillary permeability in mice , xylene-induced ear edema, carrageenan-induced rat paw edema, rat granuloma were significantly reduced. Beans gall effective component side of the mouse peritoneal capillary permeability, xylene-induced ear edema model of acute inflammation inhibition with beans bile pills original parties, refined beans gall parties fairly. Beans gall effective component side of the carrageenan-induced rat paw edema, inhibition of rat granuloma beans bile pills slightly better than the original parties and refined beans gall side. Hyodeoxycholic mice induced by acetic acid increased capillary permeability, xylene-induced ear edema, carrageenan-induced rat paw edema in rats and pigs inhibition of granuloma bile powder pretty. Hyodeoxycholic, patchouli oil alone have good anti-inflammatory effects, and the compatibility of the two drugs have synergistic anti-inflammatory effects. These results suggest that alternative hyodeoxycholic beans with bile pills in the pig powder, beans gall parties can keep anti-inflammatory effect. (2) beans gall effective component parties hyodeoxycholic, patchouli oil, refined beans gall party, pig bile powder and beans were on the original party pills OVA sensitized guinea pig ileum muscle contraction allergic reactions with rapid inhibition; rats of the same kind of passive cutaneous anaphylaxis (PCA) significantly inhibited; 2,4 - dinitrochlorobenzene induced ear skin significantly inhibited delayed-type hypersensitivity . Beans gall effective component side of the OVA sensitized guinea pig ileum muscle contraction allergic reactions to the same kind of rat passive cutaneous anaphylaxis (PCA), 2,4 - dinitrochlorobenzene induced mouse ear Inhibition of delayed-type hypersensitivity skin contour intensity and refined beans gall square, beans bile pills original parties fairly. Hyodeoxycholic right OVA sensitized guinea pig ileum muscle contraction allergic reactions to the same kind of rat passive cutaneous anaphylaxis (PCA), 2,4 - dinitrochlorobenzene induced ear skin Inhibition of delayed-type hypersensitivity considerable strength and pig powder. Hyodeoxycholic, patchouli oil alone have good anti-allergic effects, and the compatibility of the two drugs have synergistic anti-allergic effects. These results suggest that alternative hyodeoxycholic beans with bile pills in the pig powder, beans gall parties can keep anti-allergic effect. (3) beans gall effective component parties hyodeoxycholic, patchouli oil, refined beans gall party, pig powder and beans bile pills original parties could significantly enhance the mouse reticuloendothelial system phagocytosis of carbon clearance, beans gall effective component on mice carbon clearance reticuloendothelial system phagocytic function of intensity and beans bile pills original parties, refined beans gall parties fairly. Hyodeoxycholic reticuloendothelial system of mice carbon clearance phagocytic function of considerable strength and pig powder. Hyodeoxycholic, patchouli oil alone have better enhanced immune function, and the compatibility of the two drugs are synergistic and enhance immune function. These results suggest that alternative hyodeoxycholic beans with bile pills in the pig powder, beans gall parties can keep enhancing immune function. (4) beans gall effective component side, refined beans gall square test for the in vitro antibacterial test bacteria (Staphylococcus aureus, hemolytic streptococcus, beta-hemolytic streptococcus, Streptococcus pneumoniae, catarrhal cocci, Corynebacterium diphtheria, Escherichia coli, Pseudomonas aeruginosa and Candida albicans) of MIC, beans gall effective component side between 9.8 × 10-5g/mL ~ 2.0 × 10-1g/mL between refined beans gall square between 6.2 × 10-3g/mL ~ 2.0 × 10-1g/mL between, suggesting an effective component parties beans gall vitro antibacterial potency than refined beans gall Fang Qiang. Beans gall effective component side, refined beans gall side of the pneumococcal infection suspension, suspension beta-hemolytic streptococcus, staphylococcus aureus suspension mortality in mice with different degrees of inhibition, beans gall effective component side of the pneumococcal infection suspension, suspension beta-hemolytic streptococcus, staphylococcus aureus suspension mortality in mice inhibited significantly better than refined beans gall side. Tip hyodeoxycholic substitute beans bile pills in the pig powder, more conducive to its antibacterial role play. Through the split side of beans bile pills patchouli leaves and pig powder or active ingredients of effective parts, and with beans bile pills to compare the original parties, a clear beans bile pills produce clinical efficacy original parties effective parts by patchouli sesame oil and hyodeoxycholic and synergies between the two effects. Using beans bile pills active ingredient prescriptions only avoid beans bile pills original side of adverse reactions, but also radically overcome the traditional beans large bile pills, forming hard, finished product quality volatility and large technical problems and improve the technical beans bile pills content, product quality has reached the \This study defined the beans bile pills treatment of rhinitis material base, with the patchouli oil and composition hyodeoxycholic prescription, with a clear substance pharmacodynamics, modern medicine can reach the prototype for the beans bile pills laying product upgrades theoretical basis, providing a scientific basis. This research method can be used to clarify the material basis of traditional Chinese medicine to provide a scientific research ideas and methods.
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