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Patchouli oil is commonly used in traditional Chinese medicine, one with aromatic Huazhuo, appetizers vomiting, published Jieshu effect. In addition to recipes use, patchouli, or more than 30 kinds of medicine, such as \In addition patchouli oil can also be used as cosmetics, fixative agent and pesticides and other production ingredients. Is an important export product. In order to give full play patchouli oil this famous Southern medicine value, the need for quality of patchouli oil, specifications and test methods make provision. In this thesis, funded by the State Pharmacopoeia Commission of the State Pharmacopoeia 2010 edition of patchouli oil on the part of the revised supplement formulated and drafted the \The standard analytical methods meet the \provide the basis for reasonable application of the following major elements: 1, patchouli oil, patchouli oil traits of a reddish brown or greenish brown clear liquid; has a specific aroma, spicy, slightly warm. And chloroform, diethyl ether or petroleum ether miscible in any proportion. According to a 2005 edition of Chinese Pharmacopoeia requirements of patchouli method of measuring the refractive index on the basis of the original standard was revised and 12 batches of patchouli oil measured relative density, optical rotation, refractive index. Limits not revised. 2, patchouli oil TLC with patchouli patchouli alcohol and ketone Lee as control, silica gel GF254 HPTLC plates with petroleum ether: ethyl acetate: formic acid (10:0.7:0.6) as developing solvent, at a temperature of to 18 ℃, relative humidity of 30% under the conditions of the upstream expand 7 cm, remove the solvent evaporated to dryness, sprayed with 5% ethanol solution of ferric chloride color, the establishment of patchouli oil TLC method. The law increases the pogostone identification, meet the quality standards required. 3, Gas Chromatography patchouli oil patchouli patchouli alcohol and ketone levels Li established a Gas Chromatography patchouli oil, patchouli alcohol pogostone Lee quantitative analysis method, and carried out methodological inspection. On this basis, on 12 batches of different samples of patchouli patchouli oil pogostone Lee alcohol content two compounds were measured. The results showed that the quality of patchouli oil of different origin differ. Also shows a number of index components more comprehensive response to the quantitative analysis of the intrinsic quality of patchouli oil. 4, patchouli oil and gas phase chromatographic fingerprint was established by gas chromatographic fingerprint of patchouli oil. 12 batches of different origin fingerprint samples to analyze the similarity between different samples using the national development of the Chinese Pharmacopoeia Commission chromatographic fingerprint evaluation system version (2004A) calculation, the results show that each batch of patchouli oil fingerprinting Spectrum similarity greater than 0.8, each batch of samples have the same composition, but the characteristic peaks relative content distribution difference resulting chromatogram profile there are some differences. 5, patchouli ketone Preparation of our patchouli oil, patchouli Lee alcohols, ketones patchouli, go pogostone part of patchouli oil antibacterial activity were studied, the results indicate that pogostone is The main antibacterial ingredient, and has been reported pogostone with insecticidal activity, there is no mass production of the compound related news, the market has no related product sales. We patchouli oil as a raw material in production pogostone production process was studied, the results indicate that the process is short, simple equipment, low cost, easy operation, high product purity.
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