Dissertation > Excellent graduate degree dissertation topics show

Plasma Adiponectin Isoforms Distribution and Detection of Their Bioactivity

Author: LianKun
Tutor: WangHaiChang;TaoLing
School: Fourth Military Medical University
Course: Internal Medicine
Keywords: Human plasma adiponectin isoform bioactivity
CLC: R587.1
Type: Master's thesis
Year: 2011
Downloads: 39
Quote: 0
Read: Download Dissertation


BackgroundAdiponectin, an adipokine predominantly synthesized and secreted from adipose tissue, exists in 3 oligmeric isoforms: trimer, hexamer and high molecular weight complexes (containing 18-36 molecular) in human circulation. Animal experiments have demonstrated that adiponectin has 3 major biologic functions including an insulin sensitization/metabolic regulatory function, an anti-inflammatory/vascular protective function and an anti-ischemic/ cardioprotective function. Mounting clinical evidences have showed that the decreased human plasma adiponectin level is closely correlated with the risks and progression of metabolic syndrome, diabetes mellitus and cardiovascular disease. More importantly, the shift in adiponectin complexes distribution is closely associated with Thiazolidinedione (TZDs)-mediated increase of insulin sensitivity. However, a few data shows that there is no association between plasma adiponectin concentration and cardiovascular disease, or even shows a negtive association between them. A recently published data find that plasma adiponectin level is increased at the onset stage of obese/hyperlipidemic condition and then down-regulated, adiponectin bioactivity is decreased concomitantly. This result strongly hint us that total plasma adiponectin concentration only is not a comprehensive marker in response to the risk and development of disease. Therefore, we suggest that plasma levels of total adiponectin, adiponectin of various subtypes and their bioactivity are more efficient for preventing and prognosis of diseases.Aims1. The objective of our study were to compare total adiponectin and adiponectin isoform status in newly diagnosed Chinese type 2 diabetes patients with control subjects, and to identify an adiponectin isoform which is most closely correlated with disease.2. To isolate adiponectin isoforms from human plasma, establish a method to detect the bioactivity of human plasma adiponectin multimers and confirm which isoform is the most potent biological activity.Methods1. One hundred and seventy-four newly diagnosed type 2 diabetes outpatients and one hundred and eight normal people were enrolled in present study. Plasma total adiponectin was measured by ELISA. Plasma adiponectin multimers were analyzed by Western blot followed SDS-PAGE under nonheating nonreducing conditions.2. Plasma adiponectin isoforms were isolated by using ammonium sulfate precipitation, anion-exchange chromatography and gel filtration chromato graphy. 3. The bioactivity of purified adiponectin isoforms were detected by incubating human umbilical vein endothelial cells for one hour and then determining the phosphorylation level of AMP-activated protein kinase (AMPK).Results1. Comparison with healthy control people, plasma total adiponectin (P<0.0001) was signi?cantly lower in newly diagnosed Chinese type 2 diabetes patients. Furthermore, HMW adiponectin (P<0.05) was decreased in newly diagnosed Chinese diabetes patients.2. The three adiponectin subtypes isolated from plasma all activated AMPK and the HMW adiponectin was the most potent isoform.Conclusions1. Both circulating total adiponectin and plasma adiponectin multimers distribution altered in newly diagnosed Chinese T2DM subjects, HMW was down-regulated apparently. Plasma total adiponectin and adiponectin isoforms distribution may be the promising biomarkers for preventing and diagnosis of T2DM and cardiovascular disease.2. Our method efficiently purified adiponectin multimers from human plasma and preserved their bioactivity. HMW adiponectin was the most potent isoform. We also confirmed the feasibility of detection of the activity of adiponectin isoforms in human plasma.

Related Dissertations

  1. Analysis of the Isoforms of FGF8 in the Tooth of the Human and Mouse and the Effect of FGF8 on Early Tooth Development,Q954.48
  2. Study of Correlation between Serum Adiponectin Level and Arteriosteogenesis in Patients with Osteoporosis,R580
  3. Study on the Expression and Bioactivities of Porcine Interleukin-2/6 Fusion Protein in Vivo and in Vitro,S858.28
  4. Cloning, Expression and Bioactivity Analysis of Goat Interleukin-1β and Interleukin-6,S827
  5. Experimental Studies on the Different Expression of Myosin Heavy Chain in Rabbit Semimembranosus’ Sub-department,R322
  6. Effects of Telmisartan on hs-CRP and HMW-adiponectinin Patients with Essential Hypertension,R544.1
  7. A Basal and Clinical Reseach Ofω-3 Polyunsaturated Fatty Acids to Seal Non-alcoholic Fatty Liver Disease,R575.5
  8. The Relationship of Serum Adiponectin、Nitric Oxide Synthase and Carotid Atherosclerosis in Patients with Acute Cerebral Infarction,R743.33
  9. Adiponectin gene signal peptide region prokaryotic expression and ELISA detection method,R392.1
  10. Adiponectin , leptin and type 2 diabetic retinopathy correlation of,R774.1
  11. Osthole Improves Insulin Resistance and Its Possible Mechanisms in Fatty Liver Rats,R285.5
  12. 1. Improvement of tmTNF-α in Insulin Resistance of Adipocytes and Its Underlying Mechanism. 2. Construction of Conserved Sequence Deleted TNF-α Mutant and the Trimerization of Remodeled TNFRBP.,R587.1
  13. The Preliminary Study of Adiponectin Protects Against Anthracycline-induced Cardiotoxicity,R96
  14. The Changes and Influential Factors of Adiponectin、Apelin and Visfatin in Coronary Heart Disease Patients Accompanied with Abnormal Glucose Metabolism,R587.1
  15. Hyperglycemia in patients with metabolic syndrome and changes in coagulation and fibrinolysis system mechanism of rosiglitazone,R589
  16. Effect of Telmisartan and Benazepril on the Activity of Endothelial Lipase and Levels of Adiponectin in Patients with Mild Essential Hypertension,R544.1
  17. Adiponectin in Heart Failure with Normal Ejection Fraction,R541.6
  18. Atherosclerosis rat model of ADP and inflammatory factor expression and traditional Chinese medicine its control mechanism,R285
  19. Hydroxyapatite Coating Prepared on Porous Titanium Substrate by Sol-gel,R318.08
  20. The Effect of Rosiglitazone on Myocardial Protective by Ischemic Preconditioning,R965
  21. Expression of Adiponectin and Nuclear Factor Kappa B and Their Significances in Colorectal Carcinoma,R735.3

CLC: > Medicine, health > Internal Medicine > Endocrine diseases and metabolic diseases > Islet disease > Diabetes
© 2012 www.DissertationTopic.Net  Mobile