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Needle layer needle hole fungus Phellinus boydii (Phellinus baumii Pilat) belongs to rust leather volvatus Branch (Hymenochaetaceae), needle Fomes (Phellinus Quel.), Is a traditional Chinese medicines Phellinus one important source of bacteria , known as the \Mainly parasitic on lilac genus Prunus , hawthorn , and other dry on the hardwood . Traditional Chinese medicine , there are anti-tumor, protect the liver, stomach ulcers and other effects . For the further development of our precious resources Sang Wong , the paper mulberry yellow natural and artificial cultivation of wild Phellinus 95% ethanol extract of a systematic study of the chemical constituents were isolated 57 compounds. From natural Phellinus isolated and identified 50 compounds , including 11 new compounds, including four new benzyl flavanone , two new steroids, five new styryl- pyrone compounds , all new compounds structure is the use of the four joint resolution spectroscopic techniques to be validated , including infrared technology (IR), UV technology (UV), low-resolution and high-resolution mass spectrometry (MS), nuclear magnetic resonance (NMR), and the use to heteronuclear single quantum correlation (HSQC), heteronuclear multiple bond correlation (HMBC), homonuclear chemical shift correlation (COSY) two-dimensional NMR techniques (2D NMR) secondary structure analysis of new compounds . In addition , the use of liquid - Circular dichroism tandem technique (HPLC-CD) prove two benzyl dihydroflavone are a chiral enantiomer mixture, and prove that R, S configuration is unequal in nature scale exist. We have these nine new polyphenols nuclear transcription factor NF-κB inhibitory activity was evaluated and found Phellinus in polyphenols on NF-κB nuclear transcription factor does have inhibitory activity and a concentration- dependent manner. From cultured Phellinus isolated nine compounds were cultured bacteria from the first isolated .
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