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Studies on Au@PEG-ferulic Acid Antioxidant Properties and Sythesis of Watersoluble Fluorescent Probe

Author: JiangYuGang
Tutor: XuDongMing；XuYaJuan；LiuYang
School: Changchun University of Traditional Chinese Medicine
Course: Of Pharmacy
Keywords: Radical Fluorescence capture probe Nano anti-oxidants
CLC: R284
Type: Master's thesis
Year: 2011
Downloads: 54
Quote: 0
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Abstract


Biological production and scavenging of free radicals is a dynamic equilibrium, excessive free radicals can be DNA, proteins and polyunsaturated fatty acids, leading to cell damage, causing heart disease, cancer and other diseases of aging. Studies have shown that natural antioxidant ferulic acid (Ferulic acid) to remove the body of excess free radicals, free radicals caused by the treatment of a variety of diseases. However, during the application of ferulic acid but there is a poor biocompatibility, free radical scavenging and low efficiency. Nanotechnology due to its unique physical and chemical properties of these problems and provides a new strategy, our previous study found that the Trolox self-assembled nano-gold surface to obtain Au @ Trolox, its free radical scavenging effect increased 8 times, but its poor biocompatibility and potential toxicity. In response to these problems, this paper natural antioxidant ferulic acid as raw materials, the use of PEG chains do ligand to 4.5nm gold particles as a substrate, synthetic water-soluble nanoparticles functionalized Au @ PEG-Ferulic acid. 2,2 - diphenyl-generation bitterness hydrazine radicals (DPPH ·) scavenging assay showed that Au @ PEG-Ferulic acid radical scavenging rate relative to the ferulic acid increased significantly. Kinetic experiments show that, Au @ PEG-Ferulic acid DPPH · scavenging reaction two reaction rate is greater than ferulic acid, and Au @ PEG-Ferulic acid DPPH · scavenging free radical reactions, the apparent activation energy is significantly reduced. This may be due to the surface of gold nanoparticles self-assembled ferulic acid groups brought the local concentration enrichment effects and intermolecular synergy effects. Gold antioxidants has good biocompatibility, in a certain concentration range no significant cytotoxicity, and can effectively reduce the free radical induced lipid peroxidation, antioxidant for the new nano in biomedical applications opened up new avenues. Detection of free radicals is the prevention of disease and evaluate the efficacy of an important means. Ketones nitrate radical probes detect free radicals is the most direct and effective means, but such radical probes often due to the influence by its sensitivity limit its application. Classic radical fluorescence detection method is simple, low cost, intuitive phenomena, etc., but vulnerable to interference from other factors, thus affecting the detection sensitivity of the probe. For the above problem, we designed and synthesized a new class of fluorescent probes PBN N-benzyl aniline nitrogen oxides (N-(phenylmethylene) benzenamine N-oxide, NPBN), and modifications to the nano-graphene oxide (NGO) surface to obtain water-soluble nano fluorescent probes NGO-NPBN for the detection of hydroxyl radicals. UV spectroscopy (UV), infrared spectroscopy (IR), fluorescence spectroscopy (FL) NPBN other confirmed by π-π stacking surface linked to the NGO. Fluorescence experiments show that the water-soluble NGO-NPBN in water systems for hydroxyl radicals and superoxide anion better capture capability, which probes the application of a non-water-soluble put forward new ideas.

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