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Spinal cord injury (spinal cord injury, SCI) incidence has been rising with the progress of the times, 60 / million population in 2002, the incidence rate of spinal cord injury, and this data in the 1980s, only 6.8 / million population. The disease mainly against young adults, treatment, and care required to spend great, and the high morbidity and mortality, more than 50% of patients showed paraplegia, has become a modern society unmet medical problem. For spinal cord injury, worldwide currently no truly effective treatments, however, the only injury early large dose of methylprednisolone efficacy has been affirmed. However, this therapy will produce a range of side effects, and also has been pointed out that this method is really effective is debatable. The reason behind the \In addition to the the injury early primary mechanical damage, the damaged parts of the spinal cord after injury will happen a complex series of pathophysiological reactions and biochemical reactions, known as the \Secondary injury involving microcirculation, local electrolyte imbalance, lipid peroxidation, inflammation, apoptosis, and glial scar formation, and many other aspects of the current treatment for one to two mechanisms symptomatic treatment, and the effect is very Micro. The complex composition of Chinese traditional herbal compound, complex mechanisms of injury of spinal cord injury, a multi-pronged, while Chinese medicine cheap, side effects, and has been confirmed part of traditional Chinese medicine and its efficacy of the active ingredients. So important direction of the field to carry out the herbal compound material effect basic research the treatment of spinal cord injury. Objective: In accordance with the pharmacy and prescription and syndrome molecule Prescription theory to explore the basis of composition and material efficiency of the traditional Chinese medicine treatment of spinal cord injury, provide theoretical support for the development of spinal cord injury treatment drugs. Methods: The literature review related to TCM basic theories, screening related to traditional Chinese medicine. Alternative Chinese medicine are grouped in accordance with the theory of square card group parties, were performed to boiling water and ethanol extracted by TLC and HPLC methods to detect the physical content of active ingredients of the trend. Results: ① Select Salvia, Panax, and Chuanxiong for alternative Chinese medicine. ② Overall, the active ingredient in the alcohol extract extraction rate is generally higher than the water extracts. ③ water extract thirty-seven pairs extraction rate of salvianolic acid B has little effect, it was increased by 28.35% in the alcohol extract; Chuanxiong little effect on the extraction rate of salvianolic acid B; thirty-seven Chuanxiong salvianolic acid B extraction rate has little impact in the water extract, alcohol extract it increased 23.69%; ④ water extract almost free of tanshinone II А; thirty-seven enables the extraction rate of tanshinone Ⅱ А l 16.66%; Chuanxiong Tanshinone Ⅱ А extraction rate can be reduced by 57.34%; thirty-seven can mitigate the reduction in Chuanxiong of Tanshinone Ⅱ А extraction rate, that Dan participation in Chuanxiong common extract thirty-seven after Tanshinone Ⅱ А, the extraction rate of 54.97% ; the ⑤ Danshen so ferulic acid extraction rate increased by 116.91% and 64.73% respectively; thirty-seven ferulic acid extraction rate were increased in water extracts and ethanol extracts water extracts and ethanol extracts 381.94 % and 171.84%; Salvia extract in water and ethanol extracts of the ferulic acid extraction rate thirty-seven increased by 255.25% and 82.56% respectively; ⑥ Salvia allows notoginsenoside R1 lower extraction rate of the water extract 38.52%, the alcohol extract little effect; Chuanxiong notoginsenoside R1 can be in the water extract extraction rate was increased by 128.79% alcohol extract; Salvia Chuanxiong notoginsenoside R1, in water extract extraction rate has little effect, can it in alcohol extract increased 95.38%; ⑦ Salvia ginsenoside Rg1 can be reduced extraction rate of 150.49% of the water extract, the extraction rate of the alcohol extract increased 20.57%; ginsenoside Rg1 can be extracted in the water extract of Chuanxiong increased 45.38%, alcohol extract lower extraction rate of 50.52%; Salvia thirty-seven to enable the ginsenosides Rg1 in water, alcohol extracts in the extraction rate was reduced by 40.33% and 17.69%; ⑧ Salvia allows ginsenoside Rb1 water extract lower extraction rate of 53.78%, the extraction rate increased to 59.13% in the alcohol extract; the Chuanxiong allows ginsenoside Rb1 the extraction rate in the water extract increased by 44.31% and 48.25% reduction in the extraction rate in the alcohol extract; Salvia thirty-seven ginsenoside Rb1 in the extraction rate of the aqueous extract can be reduced by 50.75%, in the alcohol extract extraction rate increased to 17.39%. Conclusion: ① Select Salvia, Panax, Chuanxiong as a candidate medicine; ② recommended \Objective: I was under the experimental TCD, further evaluation of its role in the treatment of acute spinal cord injury in rats. Methods: 132 female SD rats were randomly divided into Salvia group, Panax Chuanxiong group, Salvia thirty-seven Chuanxiong group, the control group, sham operation group of six groups (n = 22). The first ig 10d each dose group, once a day, and then Allen's combat modeling, successful modeling continue gavage until killed after modeling 1d, 3d, 5d, 1w, 2w, 4w 6w, 8w line BBB score, evaluation of motor function in rats recovery. 1w, 2w, 4w, 8w rat spinal cord HE staining to check the pathological changes (n = 4), using immunohistochemical methods to check after spinal cord injury 4W groups GFAP expression changes in spinal cord tissue ( n = 4). 72 hours after injury spinal cord tissue homogenates of caspase-3, IL-1β, IL-10 content was measured by ELISA (n = 6) were detected in serum and spinal cord tissue homogenates SOD, MDA, and iNOS content. Results: Serum SOD administration group increased compared with the control group (P lt; 0.05), serum MDA, iNOS each dose group than in the control group decreased (P lt; 0.05) the remaining indicators dose group than in the control group, no statistical learning differences. Conclusion: Prescription and extraction conditions under spinal cord function recovery of each dose group than the control group did not show.
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