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Mining the QTLs for Increasing Thousand Grain Weigh from Synthetic Wheat

Author: LiaoXiangZheng
Tutor: RenZhengLong;JiaJiZeng
School: Sichuan Agricultural University
Course: Crop Genetics and Breeding
Keywords: Synthetic wheat Microsatellite markers Quantitative trait loci Grain weight
CLC: S512.1
Type: PhD thesis
Year: 2008
Downloads: 158
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Abstract


Wheat is one of the most important food crop in the world, due to the bottleneck effect and long-term options, the narrow genetic base of the modern cultivated wheat. Wild relatives of wheat there is a large number of excellent genes for wheat improvement. Therefore, to explore and use the the excellent gene in the wild relatives of wheat, is conducive to broaden the genetic base of the cultivated wheat. The weight is one of the constituent elements of the wheat production, to further improve the grain weight is an important way of super high-yielding wheat breeding. However, grain weight and grain number and panicle was usually negatively correlated positively correlated with plant height, super high-yielding wheat breeding limiting factor. In this study, tetraploid Persia wheat (2n = 28, AABB) Ae.tauschii Ae38 (2n = 14, DD) hybrid synthetic hexaploid wheat AM3 as the donor parent, common wheat Laizhou 953 recurrent parent, 5 backcross two F 2:3 Sub-segregating populations (high grain weight groups of 85 families and low grain weight groups 75 families) for the material by molecular markers, to explore the synthetic wheat increased weight of the QTL, and other agronomic traits QTL mapping, to lay the foundation for further QTL fine mapping and cloning research. The main results are as follows: 1. Using SSR fluorescent marker analysis of the SSR primers material a combination of technical and silver dye technology with the 648 pairs of different sources polymorphism in pro of the Laizhou 953 and Am3, including 348 pairs of cited material between two parents have stable polymorphic frequency of 53.7%. 66 SSR primer pairs in high-grain weight introgression lines population polymorphism, accounting for 18.96% of polymorphic markers. 63 SSR primer pairs polymorphism in the low grain weight import-based groups, accounting for 18.1% of the polymorphic markers. 2 Map Manager software, the high-grain weight import-based groups of 58 polymorphic SSR markers positioned on wheat chromosome 8, chain total length 344.6cM, the average genetic distance between each mark 5.9cm. The same way low grain weight groups of introgression lines 52 polymorphic SSR markers positioned on on wheat chromosome 9, linkage map the total length 366.9cM average the genetic distance between each mark 7.1cm. Import fragment is far greater than the theoretical value. Use Windows QTL Cartographer 2.0 software, using composite interval mapping method to take LOD ≥ 2.5 threshold, QTL detection of the two groups. 10 QTLs detected in a high grain weight groups, three grain weight QTL are located at 1A, 3D and chromosome 4B, a grain length QTL located on chromosome 7B, two QTLs for grain width are located at 1A, and chromosome 4B the two spike QTL 4B and 7B chromosome 2 QTL for plant height in 3D and 4B chromosome. To 17 QTLs detected in a low grain weight groups, three grain weight QTL in the chromosome 1a, 4B and 7B, respectively, four QTLs for grain length 1A and chromosome 2D, a grain width QTL located on chromosome 7B, 2 a spike QTL are located on chromosomes 4B and 7B, two QTL for plant height in 2D and chromosome 4B, 5 heading date QTL are located at 2A, 2D, 6B and 7B chromosome. 4 groups of high grain weight introgression lines were detected to three from the the Am3 increase in grain weight QTL loci (QGw.caas-1A1 QGw.caas-3D, QGw.caas-4B1). QGw.caas-3D detected in three environments, 18.1% -31.8% of the phenotypic variation explained, increased weight 2.3-4.8 g, indicating that the QTL is a main effect QTLs. QGw.caas-1A1 detected in the two environments, phenotypic variation explained 21.4% -33.8%, increase grain weight 2.7-3.8 g. QGw.caas-4B1 detected in the two environments, 10.9% -30.2% of the phenotypic variation explained by increased dry weight 3.9-4.8 g. Found increased grain weight QTL with spike, grains per spike and plant height there is no adverse relationship conducive to grain weight, spike, grains per spike synchronization to improve and reduce plant height, therefore is an ideal increased grain weight QTL. 5 the recurrent parent The Am3 agronomic traits than Laizhou 953 poor, but in the import-based groups, phenotypic selection and QTL analysis to elect some agronomic traits than the recurrent parent Laizhou 953 introgression lines have improved more. Such as high grain weight introgression lines IL6 average grain weight was fed with 60.5 g of the average panicle 53.3, average height 83.6cm, panicles per plant 5.8 83.6; grain introgression lines IL39 average grain number, grain weight 45.9g average spike 5.3, height 84.3cm. These introgression lines have been distributed to wheat breeding units for the genetic improvement of wheat varieties, breeding new varieties of wheat that contains synthetic wheat favorable gene / QTL.

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