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Experimental Study of Korean Medicine "Qianjinwenwutang" on Diabetic Nephropathy

Author: LinHao
Tutor: JinMingYu
School: Yanbian University
Course: Traditional Chinese Medicine
Keywords: North Korea Medical 1000 Jinwen Wu Tang Diabetic nephropathy Treatment Rats Animal models
CLC: R29
Type: Master's thesis
Year: 2011
Downloads: 16
Quote: 0
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Abstract


Objective: clear toward the medical side \Methods: Male Wistar rats were 130, of which 120 rats underwent left kidney; 10 rats underwent sham operation as a normal control group. A week after surgery nephrectomized rats by intraperitoneal injection of streptozotocin (STZ) (Streptozocin STZ) 55mg/kg normal control rats by intraperitoneal injection dose of citrate buffer. After 72h 24h urine measuring urine output and urine creatinine (Urine creatinine, Ucr), fundus vein blood fasting glucose, serum creatinine (Serum creatinine, Scr); formula Ucr / ScrX urine volume per minute, calculated creatinine clearance ( Ceatinine clearance rate, Ccr), I hematuria specimens frozen to save under test. Blood glucose ≥ 16.7mmol / L, 24h urine volume ≥ 80ml, Ccr ≥ 1.5ml/min rats finds the DN model was established. The modeling of 50 rats were randomly divided into five groups, respectively, for the model group, the positive control group, 1000 Jinwen Wu Tang high-dose group (referred to as the high-dose group), thousands Jinwen Wu Tang in dose group (referred to as the middle dose group), daughter Wenwu soup small dose group (referred to as the low-dose group), coupled with the normal control group, a total of six groups, each group of 10. Modeling weight were measured after 24 h urinary protein excretion, BUN (Blood Urea Nitrogen, BUN). Large, medium and small dose group were given one thousand Jinwen Wu Tang 10g/kg · d 5g/kg · d 2.5g/kg · d, the positive control group were given the Lotensin 10mg/kg · d, model group with the normal control group were given distilled water 10ml/kg · d. Body weights were measured again after consecutive 30d, remove the right kidney determination of the weight of the right kidney index; urine samples was measured 24h urine output, 24h urinary protein excretion Ucr fasting glucose blood specimens, Scr, BUN, calculated using the above formula Ccr. The experimental data were expressed as mean ± standard deviation (x ± s), said data processing using GraphPad Prism 5.0 software, statistical methods using single-factor analysis of variance (One way ANOVA), P lt; 0.05 was considered statistically significant. Results: ① weight and right kidney index: normal control rats after administration weight increased significantly compared with the model group, a significant difference (P lt; 0.01); after the administration of the positive control group with large, medium and small dose group body weight were significantly reduced compared with the model group, no significant difference (P gt; 0.05). Model group, the right kidney after the administration of the index was significantly greater than the normal control group, with a significant difference (P lt; 0.01); positive control group and large, in the low-dose group right kidney index with the model group, no significant differences (P gt; 0.05). The ② 24h urine output: modeling of 24h urine output of the model group was significantly more than the control group, with a significant difference (P lt; 0.01); after the administration of large, 24h urine output, small dose group was significantly less than the model group , there is a significant difference (P lt; 0.05, P lt; 0.01); 24h urine positive control group compared with model group, no significant difference (P gt; 0.05). (3) 24 h urinary protein excretion: 24h urine protein modeling model group is higher than the normal control group, with a significant difference (P lt; 0.05); 24h urine protein after administration of the positive control group and large, medium and small dose group quantitative model group were significantly lower, with a significant difference (P lt; 0.01). Comparison groups before and after administration of urinary protein reduce the value 24h urinary protein increased in the model group, while the other group of 24h urinary protein decreased;, in the low-dose group compared with the model group, a significant difference (P lt; 0.05, P lt; 0.01), while the positive control group and the model group no significant difference (P gt; 0.05). ④ blood sugar: modeling, model group, blood glucose was significantly higher than the normal control group, with a significant difference (P lt; 0.01). After the administration of a large, blood sugar, a small dose group were significantly lower than the model group, with a significant difference (P lt; 0.05, P lt; 0.01); blood sugar of the positive control group compared with model group, no significant difference ( P gt; 0.05). ⑤ BUN, Scr, Ccr: modeling and model group BUN higher than the normal control group, with a significant difference (P lt; 0.05); administration after the positive control group and large, middle dose group BUN were lower than the model group, a significant difference (P lt; 0.05, P lt; 0.01); small dose group compared with model group, no significant difference (P gt; 0.05). Model after model group Scr, Ccr were significantly higher than the normal control group, a significant difference (P lt; 0.05); big after administration, in the low-dose group Scr, Ccr obvious were lower than the model group, with significant difference (P lt; 0.05, P lt; 0.01); positive control group compared with model group, no significant difference (P gt; 0.05). Conclusion: towards the medical side, \therapeutic effect.

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