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A New Cell Type in Rat Organ of Corti after Aminoglycoside Insult

Author: WangYuCheng
Tutor: WangZhengMin
School: Fudan University
Course: Otorhinolaryngology
Keywords: Rats The organ of Corti New cells Neomycin Amikacin The Hortega s silver carbonate method TEM Reverse transcriptase polymerase chain reaction
CLC: R764
Type: PhD thesis
Year: 2004
Downloads: 73
Quote: 1
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Abstract

Objective: The new cells after rat cochlea drug injury microglial cells, atypical cells (atypical cell) to compare and extension study to explore the representation of new cells and its origin, fate, and neural stem cells or nestin () precursor cells. : Classic microglia special staining methods - silver staining (Hortega's the silver carbonate Law) new cells after rat cochlea drug injury; through TEM Marc Lenoir and Philippe Vago experimental animal models \neural stem cells or nestin () the relationship of the precursor cells for further study. Results: microglia special silver staining under light microscope observations show: as a positive control rat brain slices the microglial with the classical histological description distribution, morphology consistent, while the the cochlear central axis slice there was no similar to coloring microglia cells. Marc Lenoir and Philippe Vago experimental animal models, the term \juvenile and adult), Wistar rats (juvenile and adult) and neomycin, amikacin drugs, a variety of cross-comparison model are repeated, and ultrastructure were no significant differences. Cochlea neural stem cell marker nestin expression of nestin expression was still positive in the drug group of 16-day-old rat cochlea, the same as the normal control group nestin expression was negative. Conclusion: silver staining does not support the new cells are microglia hypothesis; combined with previous immunohistochemistry (leukocyte common antigen LCA, CD45) negative results of the research that initially ruled out the possibility of microglia. Exclude the possibility of the same cell and Marc Lenoir and Philippe Vago experimental animal models of atypical cells (atypical cell). That the new cells in the rat cochlea cochlear degree of development of imperfect correlation has a wider representation. The earliest time for the 4th day of the course of medication. Support the new cell is a corollary of the previous studies have not found a class of non-apoptotic cells. Morphology with another type of high electron density cells differentiated. That the source of new cells may be some kind of nestin () precursor cells or neural stem cells, combined with the past, we Brdu results of the study speculated that the mechanism may be generated mitosis or transdifferentiation.

A New Cell Type in Rat Organ of Corti after Aminoglycoside Insult

Abstract

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