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HO/bilirubin System in ASR and Effects of BushenTiaojing Recipe on Them

Author: MaHuiRong
Tutor: DuHuiLan
School: Hebei Medical University
Course: Traditional Chinese Medicine
Keywords: To Bushen Tiaojing parties ASR MDA T-AOC Ovarian granulosa cell apoptosis caspase-3 Ovarian
CLC: R285
Type: PhD thesis
Year: 2005
Downloads: 67
Quote: 0
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Abstract


Objective: The topic of normal rats and androgen sterilized rats ( androgen induced sterile rats ASR) as the object of study , while applying a drug intervention of HO inducers - hemin ( hemin ) for normal female SD rats the mouse HO / of bilirubin systems and the impact of ovarian function , the ASR ovarian HO / bilirubin system and kidney to regulate menstruation party ASR ovarian HO / bilirubin system ; Hemin normal SD rat ovarian HO / bile the red pigment oxidation antioxidant , anti - oxidation function and kidney to regulate menstruation square antioxidant function of ASR ovarian HO / bilirubin system ASR ovarian HO / bilirubin system ; Hemin ovaries of HO / bilirubin anovulatory occurrence with ovarian HO / bilirubin and kidney to regulate menstruation side the ASR ovarian HO / bilirubin ; observed rats ovarian sex hormone levels , ovarian morphological changes , ovarian weight index changes , in order to explore the the HO / of bilirubin systems with anovulatory relationship and kidney to regulate menstruation party its impact . A systematic study of the normal rat ovarian HO / bilirubin normal rat ovarian HO-1 activity and serum bilirubin with 3 to 4 months of age 20 healthy female SD rats were randomly divided into 2 group. Hemin group cervical subcutaneous injection hemin, dose 30μm/kg; neck back normal group injected with normal saline for seven consecutive days . Vaginal smears , to select metoestrum killed , by colorimetry , observation and comparison of the rat ovary HO-1 activity and serum Tbil , Ibil and Dbil the change . Normal rat ovarian HO / bilirubin oxidation, anti-oxidation ability animal groups, administration , killed the same , by colorimetry , uniform observed compared serum ( plasma ) and ovarian tissue changes of pulp in the MDA, T-AOC . And normal rat ovarian HO / bilirubin oxidation , anti- oxidation ability to analyze the relationship . Ovarian teeth apoptosis 3.hemin on normal rats affect animal grouping , administration , and killed the case with the former , using immunohistochemical staining , observation and comparison group rat ovarian granulosa cell apoptosis and caspase-3 protein expression circumstances change and its relationship with the ovarian granulosa cell apoptosis . Normal rat ovarian HO / bilirubin ovarian function

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