Dissertation > Excellent graduate degree dissertation topics show

The Role of Cx43 Gene in Myocardialization of Cardiac Proximal Outflow Tract Septum in Mice

Author: ZhaoXiaoQing
Tutor: HuangGuoYing
School: Fudan University
Course: Pediatrics
Keywords: Heart development Mice Cx43 Knockout Outflow tract septum Myocardialization Neural crest cells Apoptosis TGFβ2
CLC: R725.4
Type: PhD thesis
Year: 2005
Downloads: 71
Quote: 1
Read: Download Dissertation

Abstract


Cone cadres malformations are common complex congenital heart disease (CHD ) , is one of the main cause of death in children . The proximal outflow tract plays a vital role in the development of the cone cadres . Proximal outflow tract septum is the level of semilunar valve and flap outflow tract separated , this interval was initially formed by the crest of the endocardial mesenchymal form of a pad tissue . In the the heart development process , the proximal outflow tract septum stroma myocardial ingredients , this process is called \The process of myocardialization is an important event in the development process of the outflow tract , myocardialization disorder can lead to structural and functional abnormalities of the outflow tract , therefore clarify the outflow tract septum myocardial formation process will help to further reveal the outflow tract abnormalities in the pathogenesis . Participate in or influence myocardial process possible cellular and molecular mechanisms have been proposed , in which the cardiac neural crest cells , apoptosis and transforming growth factor (transforming growth factor, TGF) β2 gradually attracted people's attention . It also found that in recent years connexin 43 ( Conecxin , Cx ) is closely related with the development of the embryonic heart cone cadres . Cx43 deletion and overexpression model showed right ventricular outflow tract abnormalities and obstruction, and cardiac neural crest cells may be involved in its pathogenesis . Therefore , the subject of the use of the Cx43 gene knock in addition to ( knockout , KO ) mouse model to study the role of Cx43 in myocardial process and its possible mechanism ; to further reveal cone ' of Cx43KO mice deformity pathogenesis . The purpose of the first part of normal mouse cardiac outflow tract septum myocardial process studies to explore the heart proximal outflow septum and temporal variations of the myocardial process , as well as cardiac neural crest cells , apoptosis in this process possible role . Materials and Methods adult 2 months of age the C57/BL6 mice mating , the choice of gestational age ( embryonic day , E ) 11.5 days ( day , d ) day after birth in mice for the study . By immunohistochemical method for the determination of striated muscle actin alpha - SCA ( of alpha - Sarcomeric Actin ) , AP -2a ( Activator protein - 2a ) , a marker of neural crest cells , apoptosis - related molecules Active caspase - 3 expression ; using TUNEL ( terminal deoxylnucleotidyl transferase mediated-dUTP nick end labeling) in situ detection of cell apoptosis extent .

Related Dissertations

  1. The Study on Cryopreservation and Mechanism of Freezing Injury on the Spermatozoa of Coelomactra Antiquate,S968.3
  2. The Anti-tumor Effect of CADPE and Inducing Apoptosis in Human Gastric Cancer Cells,R735.2
  3. Cloth and β- elemene combined administration of anti-tumor effect and mechanism of celecoxib,R96
  4. Study of Sepia Ink-Astragalus Preparation for Relieving Damage Caused by Chemotherapy,R285.5
  5. TRAIL in the regulation of tumor invasion CD4 ~ CD25 ~ Treg,R730.2
  6. Effects of Angiogenic Factor on Follicular Angiogenesis and Development in Immature Mouse,S865.13
  7. The Study of Lead Exposure on Cyprinus Carpio Ovary Epithelial Cells,X174
  8. Study on Gene Expression of β-defensin1 in Mammary Gland of Mice,S852.4
  9. Effects of Diclazuril on G3PDH in Second-generation Merozoites of Eimeria Tenella,S858.31
  10. The Effects and Preliminary Study of PCV2 on Ca2+ Signal in Lymphocytes of Piglets,S858.28
  11. Effects of Angiogenic Factor on Follicular Angiogenesis and Development in Sexual Maturity Mice,S852.2
  12. Expression of β-Catenin in Pig’s Ovary and the Effect of β-Catenin on Porcine Granulosa Cells Apotosis and Steroidogenesis Related Enzyme,S828
  13. Roles of MMP-7 and Lysozyme in the Pathogenesis of Ulcerative Colitis Induced by DSS in Balb/c Mice,S858.91
  14. Induced Combination Apoptosis by FB1 and AFB1 in Vero Cell,S856.9
  15. Some Pharmacology and Toxicity of Three Prescriptions from Chinese Veterinary Pharmacopoeia,S859.5
  16. The Apoptosis Mechanism Induced by Aflatoxin B1 and Deoxynivalenol in Primary Hepatocyte of Cyprinus Carpio,S856.9
  17. Effects of BMPR-IB Gene Silencing by Small Interfering RNA on Apoptosis of Porcine Pollicular Granulosa Cells and Ecpression of BMP Pathy-Way-Ralted Genes,S828
  18. The Effects of Don on Proliferation, Differentiation, and Apoptosis of Chondrocytes in Chicken,S858.31
  19. The Effect of Foxol on Apoptosis of Mouse Granule Cells,S865.13
  20. Study of Melittin on Tumor Inhibition and Part of Mechanism of Human Hepatocellular Carcinoma HepG-2 Xenograft in Nude Mice,R735.7
  21. Apolipoprotein E Gene Knock-out and High-fat Diet on IP3、 IP3R-1 ＆ Aβ Expression in Neurons of Mice’s Hippocampal CA1 ＆ Ca3,R329

CLC: > Medicine, health > Pediatrics > Children within the science > Pediatric cardiac and vascular disease
© 2012 www.DissertationTopic.Net  Mobile