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Studies on the Preparation and Bioactivities of Peptides from Swine Blood

Author: FangJun
Tutor: LuXiangYang；QiuYeXian；LiZongJun
School: Hunan Agricultural University
Course: Botany
Keywords: A32 Strain Fermentation PSB Toxicology Biological activity
CLC: TQ464.5
Type: PhD thesis
Year: 2006
Downloads: 854
Quote: 11
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Abstract


About five hundred million swine were killed in our country every year. It was about 45% of the world’s. The blood of swine was very abundant. At present, it wasn’t utilized on the whole except that a little was used as soybean curd, blood bowels and food. It not only wasted the sources but also severe contamination surroundings. It was studied in this topic that fresh swine blood was fermented to get peptides by A32 strain. At the same time, it was studied that bioactivities of PSB such as antioxygen, norblood-fat and renforcing immune function. Main results were reported as follows:1. The filter and separation of fermentable blood meal strainsBy utilizing plate streaking, A32 was gained from soil of individual slaughter house in countryside. The strain could grow prosperity on medium with swine blood as only nitrogen source and the enzyme activity of its protease was high. Utilizing A32 to ferment swine blood, the protein of power from swine blood decreased 28.7%, the nitrogen of free amino acids increased 4.20 times and dissolving protein increased 60%. All these demonstrated that A32 was a fine strain to ferment swine blood.2. Appraisement of strainPhysiology and biochemistry appraisement: Gram staining was negatively and it had germ. Vivo of thalline was tint, the surrounding of thalline was purple circle. The strain was initial appraised as bacillus.Molecular biology: Refer 16s rRNA sequence to GenBank and contrasted in Blast, it was appraised as bacillus subtilis through homologization sequence comparison.3. The condition of toco-protease of A32 strainThe condition of toco-protease was studied on various factors and orthogonal experiments. The optimum fermentation medium containing 2% soybean powder, 3% cornmeal, Na2HPO4·12H2O 0.4%, KH2PO4

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