Dissertation > Excellent graduate degree dissertation topics show

Proteomic Analysis of Different Phenotype of Hepatocellular Carcinoma and Textual Research of S100A9

Author: ChenBin
Tutor: PengMinHao
School: Guangxi Medical University
Course: Oncology
Keywords: human hepatocellular carcinoma tumor invasion tumor recurrence two-dimensional electrophoresis matrix assisted laser desorption/ionization time of flight mass Spectrometry S100 calcium binding protein A9 (calgranulin B)
CLC: R735.7
Type: PhD thesis
Year: 2006
Downloads: 185
Quote: 0
Read: Download Dissertation


Objective Using a comparative proteomic approach, we screened key proteinic molecules correlated with invasion and recurrence by comparing the difference in protein expression of different phenotype of human hepatocellular carcinoma (HCC) tissues. Methods 5 cases of invasive HCC and 5 cases recurrent HCC tissues proved by clinical and pathological examination were divided into two groups(contrasted with 5 cases non-invasive and non-recurrent HCC tissues, respectively). Totalprotein extracted from the specimens of these groups were separated by two-dimensional gel electrophoresis (2-DE). IPG drystrip (13cm, pH3~10 non-linear) was used in the first dimensional IEF electrophoresis, and 12.5% SDS-PAGE for the second dimensional vertical electrophoresis. The preparative 2-DE gels stained with Coomassie brilliant blue were scanned using ImageScanner and analyzed with ImageMaster 2D Platinum 5.0 software. Selected differential protein spots (showing 2 or more than 2 fold difference and more than 50% emerging frequency) of comparative groups were digested into peptides by trypsin in-gels. Then these peptides were assayed by MALDI-TOF MS. Data were inputted the searching engine MASCOT (Matrix Science) against the NCBI non-redundant protein sequence database and homologous protein could be identified. Validation procedures for the screened protein S100A9 were then carried out by immuno-histochemistry, reverse transcription polymerase chain reaction analysis (RT-PCR) and western blot assay. Expressions of S100A9 in different type of liver tissue specimens were observed by immunohistochemistry. The

Related Dissertations

  1. Cellular and Proteomic Analyses of Endocytosis Mechanism Involved in Pollen-tube Growth in Nicotiana Tabacum,Q942
  2. Proteomic Analysis of Vibrio Alginolyticus in Response to Four Types of Antibiotics,S941
  3. Salt-Tolerance Evaluation of Brassica Napus Germplasm in the Bud and Seeding and Establishment of the Two-Dimensional Electrophoresis System,S565.4
  4. Isollation and Identification of Haemophilus Parasuis and Its Proteomic Analysis under in Vitro Growth Conditions Mimicking the in Vivo Environment,S858.28
  5. Mycobacterium smegmatis MSMEG 6 402 gene function,R378
  6. Analysis of Antenna Protein of Silk Moth by Two -dimensional Electrophoresis,Q966
  7. Correlation of Shh and Ptch Expression with Invasion and Tumor Recurrence After Radical Surgery Resection of Hilar Cholangiocarcinoma,R735.8
  8. Screening of Differential Proteins in Different Parts of Pedicle Periosteum of Sika Deer,S825
  9. Study on 2-D Electrophoresis of Total Protein and Its Antibacterial or Other Main Function of Sarcophaga Peregrina’s Endochylema,Q966
  10. Studies on Physiological Mechanism and Preliminary Proteomics of Buchloe in Response to Temperature Stress,S688.4
  11. Study on the Differential Proteomics Expression of Glioblastoma Cell Lines Derived from Chinese,R739.4
  12. 1.Proteome Comparative Analysis of Low-Grade Human Brain Astrocytomas and AdjacentNormal Gliomas 2.Proteome Comparative Analysis of Hela Cells Transfected with TRIM45 Gene,R739.41
  13. Expression of Ezrin, MMP-9 and MVD in Gastric Carcinoma and Its Significance,R735.2
  14. LRRK2 mutation body induced dopaminergic neuronal apoptosis preliminary study of proteomics,R742.5
  15. The Model of Skeletal and Cartilage Malformation Induced by the Intraperitoneal Administration of Deoxynivalenol in Mice and the Screening of Relating Distinct Proteins,R682
  16. UV Mutation of Pectinase-producing Aspergillus Niger and Proteomic Analysis of Synthetic Regulatory Mechanisms,TQ925
  17. The Proteomics Research of Virescent Mutant of Cucumber Leaf,S642.2
  18. Physiological Characteristics of Cold Resistance and Proteonmic Analysis of Proteins for Cold Stressed Winter Rapa (Brassica.Rapa) by Two-dimensional Electrophoresis,S565.4
  19. Expression and Implication of Human CD44 Variant in Laryngeal Squamous Cell Carcinom,R739.65
  20. Studies on E-cadherin Expression Inducement of Prostaglandin E2 in HepG2 Cells and Its Molecular Mechanism,R735.7
  21. Effects of Caveolin-1 siRNA-mediated Silencing of Caveolin-1 on Mouse FBJ-S1 Cells and Studies on GD1a-binding Molecules as Revealed by Immunoprecipitation,R73-3

CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Liver tumors
© 2012 www.DissertationTopic.Net  Mobile