Dissertation > Excellent graduate degree dissertation topics show

Differentially Expressed Proteomics during Carcinogenesis and Progression of Human Hepatocellular Carcinoma

Author: LiShan
Tutor: LuoGuoRong;LiuYinKun
School: Guangxi Medical University
Course: Human Anatomy and Embryology
Keywords: human hepatocellular carcinoma carcinogenesis tumor progression two-dimensional electrophoresis matrix assisted laser desorption/ionization-time of flight mass spectrometry annexins A4 (ANXA4) PeroxiredoxinⅡ(PrxⅡ) PeroxiredoxinⅢ(PrxⅢ) RNA-mediated interference
CLC: R735.7
Type: PhD thesis
Year: 2006
Downloads: 402
Quote: 2
Read: Download Dissertation

Abstract


Hepatocellular carcinoma (HCC), of which the morbidity and mortality are in the forefront among the overall tumors worldwide, is one of the malignancies with difficult in early diagnosis and extremely poor prognosis. The formation of HCC is a multistep, multistage and multifactor process like most other tumors. The conventional strategy of research on single gene has been challenged by the rapidly progress of proteomics, which can be utilized to observe the dynamical changes of nature and quantity of total protein during progression of disease. Proteomics can be used to find new protein markers and key molecules and therefore is an effective and high-throughout method for exploring mechanism of disease and for searching targets of diagnosis or therapy. By optimizing the technical platform of two-dimensional electrophoresis (2-DE), in the present study we observed dynamically the differentially expressed proteins of liver tissue during carcinogenesis and progression of human Hepatocellular Carcinoma,which including normal liver tissue, chronic type B hepatitis tissue, cirrhotic tissues, HCC tissue.We got a group of significant differential proteins, and ANXA4、 PrxⅡ、PrxⅢproteins which may be related to hepatocarcinogenesis was further confirmed by Western blot and RT-PCR. We also drew a positive conclusion through further analysis and validation of the biological function of ANXA4 with RNA interference technique and some following experiments related to cell properties of carcinogenesis. The entire study included four parts as following.

Related Dissertations

  1. Cellular and Proteomic Analyses of Endocytosis Mechanism Involved in Pollen-tube Growth in Nicotiana Tabacum,Q942
  2. Proteomic Analysis of Vibrio Alginolyticus in Response to Four Types of Antibiotics,S941
  3. Salt-Tolerance Evaluation of Brassica Napus Germplasm in the Bud and Seeding and Establishment of the Two-Dimensional Electrophoresis System,S565.4
  4. Isollation and Identification of Haemophilus Parasuis and Its Proteomic Analysis under in Vitro Growth Conditions Mimicking the in Vivo Environment,S858.28
  5. Mycobacterium smegmatis MSMEG 6 402 gene function,R378
  6. Analysis of Antenna Protein of Silk Moth by Two -dimensional Electrophoresis,Q966
  7. Screening of Differential Proteins in Different Parts of Pedicle Periosteum of Sika Deer,S825
  8. Preliminary Study on STAT2 Significance in Cervical Carcinogenesis,R737.33
  9. Study on 2-D Electrophoresis of Total Protein and Its Antibacterial or Other Main Function of Sarcophaga Peregrina’s Endochylema,Q966
  10. Studies on Physiological Mechanism and Preliminary Proteomics of Buchloe in Response to Temperature Stress,S688.4
  11. Study on the Differential Proteomics Expression of Glioblastoma Cell Lines Derived from Chinese,R739.4
  12. 1.Proteome Comparative Analysis of Low-Grade Human Brain Astrocytomas and AdjacentNormal Gliomas 2.Proteome Comparative Analysis of Hela Cells Transfected with TRIM45 Gene,R739.41
  13. LRRK2 mutation body induced dopaminergic neuronal apoptosis preliminary study of proteomics,R742.5
  14. The Model of Skeletal and Cartilage Malformation Induced by the Intraperitoneal Administration of Deoxynivalenol in Mice and the Screening of Relating Distinct Proteins,R682
  15. UV Mutation of Pectinase-producing Aspergillus Niger and Proteomic Analysis of Synthetic Regulatory Mechanisms,TQ925
  16. The Proteomics Research of Virescent Mutant of Cucumber Leaf,S642.2
  17. Physiological Characteristics of Cold Resistance and Proteonmic Analysis of Proteins for Cold Stressed Winter Rapa (Brassica.Rapa) by Two-dimensional Electrophoresis,S565.4
  18. Studies on E-cadherin Expression Inducement of Prostaglandin E2 in HepG2 Cells and Its Molecular Mechanism,R735.7
  19. Effects of Caveolin-1 siRNA-mediated Silencing of Caveolin-1 on Mouse FBJ-S1 Cells and Studies on GD1a-binding Molecules as Revealed by Immunoprecipitation,R73-3
  20. Proteomics Analysis of Cerebral Cortex in Pentylenetetrazol (PTZ)-induced Kindling Models Made by A1 Adenosine Receptor Knock-out Mice,R742.1
  21. Analysis of Components in Bronchoalveolar Lavage Fluid of Rats Following Silica Exposure,R135.2

CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Liver tumors
© 2012 www.DissertationTopic.Net  Mobile