Dissertation > Excellent graduate degree dissertation topics show

Mechanism of mast cell function on the effect of acupuncture analgesia and stimulate the sensitivity of physical TCM treatment process

Author: ZhangDi
Tutor: DingGuangHong
School: Fudan University
Course: Biomechanics
Keywords: Mast cells Cromolyn sodium Analgesic effect of Physical stimulation Degranulation Mechanical force The course of treatment Thermally stimulated Cell function Cell membrane
CLC: R245
Type: PhD thesis
Year: 2007
Downloads: 203
Quote: 0
Read: Download Dissertation


OBJECTIVE;To better understand the function of mast cells in acupuncture analgesia and test their sensitivity to mechanical,thermo and light as the physical stimulations during traditional Chinese medicine treatment in acupoints.METHODS;We made analgesia measurements by tail flick model and observed tissue slices of mast cell about density and degranulation ratio before/after acupuncture in animal experiment,and also,we used patch-clamp technique to investigate the activation of human mast cell(HMC-1 cell line)by mechanical stress or noxious heat and optically observed the degranulation phenomenon of mast cell in response to laser light.RESULTS;1.The manual stimulation by acupuncture at ST36 of rat resulted in analgesia and the effect was more pronounced than after stimulation of a sham point nearby the acupuncture point;2.The density of mast cells from ST36 of rat was higher than from nearby sham point,and acupuncture caused a remarkable increase in degranulation of the mast cells;3.Pretreatment of disodium chromoglycate(mast cell stabilizer)into acupuncture point not only counteracted the phenomenon of degranulation,but also impaired the analgesic effect of acupuncture;4.In the whole-cell patch-clamp configuration,increasing hydrostatic pressure(-30 to -90 cm H2O applied via the patch pipette)induced a current that could be inhibited by 10μM of the specific inhibitor of TRPV2 ruthenium red(RuR).5.Temperatures above 50℃, that are reached during moxibustion,induced a similar RuR-sensitive current.6.Laser light of 640 nm applied to acupoints had been shown previously to be highly effective in analgesia.20 min of light application at 48 mW induced pronounced degranulation in HMC1,which could be blocked by 0.02g/ml DSCG as well as by the TRPV2 inhibitor RuR.CONCLUSIONS;We found that mechanical stimulation of acupoints is associated with mast cell degranulation and that mast-cell degranulation can be correlated with acupuncture-induced analgesia.We suggest that TRPV2 plays a key role in mast-cell degranulation directly in response to mechanical and moxibustion stimulation as well as laser-light forming a facilitating step in acupoint-stimulated analgesia.

Related Dissertations

  1. Fabrication of Membrane Electrode Assembly for PEMFC,TM911.4
  2. Identification and Robustness Analysis of Nonlinear Hybrid Dynamical System Concerning Glycerol Transport Mechanism,TQ223.162
  3. The Function of Fuantang Treating POI in Mice Model and the Mechanism Related with Mast Cell,R285.5
  4. Myristoylation and Plasma Membrane Binding Regulate Tobacco NtRab5b Transcription,Q943
  5. Efects of Obesity on Insulin Resistance, Endothelial Function and β Cell Function,R587.1
  6. Study on TCR Vβ Subfamily Expression and the Function of T Cells in Patients with Lung Cancer,R734.2
  7. Huanglianjiedu Tonga flavor particles combined basal insulin , metformin onset obesity, type 2 diabetes clinical observation,R587.1
  8. The Effects of AMF on Cell Membrane Endogenous Polyamines and Salicylic Acid in Citrus under Drought Stress,S666
  9. Effect of Continuous Blood Purification on Prognosis and Endothelial Cell Function in Patients with Severe Sepsis,R459.7
  10. Dynamic observation of short-term intensive insulin therapy on patients with newly diagnosed type 2 diabetes islet β cell function,R587.1
  11. (Study on Structural and Functional Characteristics of Neural Cells Orientation Originated in Bone Marrow Stromal Cells),R329
  12. Study on Anti-inflammatory, Antipyretic and Analgesic Effect of Total Flavones from Lagotis Brevituba Maxim,R29
  13. Chinese intervention inflammatory cytokines diabetes islet β -cell function to protect Clinical and Experimental Research,R259
  14. To Obsever the Short-term Clinical Therapeutic Effect of Chronic Soft Tissue Injury Pain Treated with the Elbow Naprapathy,R274.9
  15. Research on Screening, Extraction and Mechanism of Active Materials in Cosmetic Anti-allergy and Anti-irritation,TQ652
  16. Research of Electret Meloxicam Patch on the Drug Transdermal Enhancement in Rats and Biosecurity,R94
  17. Studies on Charge Storage Stability of Electret Transdermal Patches and Mechanism of Drug Transdermal Transfer,R94
  18. Effects of Fluctuant High Blood Glucose on Islet β-cell Function and β-cell Apoptosis in Gk Rats,R587.1
  19. Wild papaya extract analgesic effects of,R285
  20. Research on the Mechanism of Stomach Intestine Dynamia and Visceral Sensation of Zhongyao on IBS-D Rats,R285.5
  21. Clinical Studies and Mechanism of the Li Chang Yin in Treating of Diarrhea-predominant Irritable Bowel Syndrome,R259

CLC: > Medicine, health > Chinese Medicine > Chinese medicine clinic > Acupuncture, acupuncture therapy
© 2012 www.DissertationTopic.Net  Mobile