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Electrochemical sensors used in immunoassay research

Author: ChaiXiaoSen
Tutor: ZhouJia
School: Fudan University
Course: Microelectronics and Solid State Electronics
Keywords: Electrochemical Integrated chip Nanoelectrodes Graphene Immunosensor
CLC: TP212.2
Type: Master's thesis
Year: 2011
Downloads: 62
Quote: 0
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Abstract


The electrochemical sensor is based on an electrochemical principle and the electrochemical characteristics of the substance to be detected to be tested, it is very sensitive for the detection of trace substances characteristic. Electrochemical method for detection of antigen-antibody, can provide real-time test results for disease diagnosis, environmental toxins detection and drug screening. Conducted various research papers on the electrochemical sensors used in immunoassay: electrochemical sensor chip, multi-channel on-chip integrated new structure based on the theory of micro-electrode electrochemical immunoassay designed a concentric Electrochemical chip arranged in a multi-channel chip integrated new structure, and studied the scale of not disc working electrode, different electrode arc and the radius of the role of the sensor signal, has been optimized for high sensitivity integrated chip. The same as the size of the working electrode, radians to 3π / 2, the auxiliary electrode having the largest peak current, peak increase of 35%; peak current is increased with the increase of the radius of the auxiliary electrode. The radius of the working electrode is 500μm, the radius of the auxiliary electrodes is 200μm, 3π / 2 radians, the oxidation peak 2.14μA. 2, the electrode surface nanostructures modification of research based on the the dramatic diffusion theory of Au and Cu at high temperatures achieved by annealing process gold electrode surface nanostructures to study the formation of nanostructures under different temperature and time. When the nanostructures scale at 100-200nm, compared to the same area of ??the plane electrode, the increase in the peak current 0.5μA, the peak current, has a good linear relationship with the square root of the scan rate of the Faraday current characteristic. Fitting linear degree variance R2 = 0.9992, slope 9.1935, higher than the the bare electrode linear fit slope 8.4633. This structure electrochemical response increased with the scan rate is more sensitive. Nanocarbon material surface modified electrodes with SAM self assembling method nanomaterials SGNF (Stacked graphene nanofibers) modification to the electrode, constitute nano SGNF / SAM / Au modified electrode. Electrochemical characterization device SGNF / SAM / Au electrode than Au electrodes, and Graphite, / SAM / Au electrode greater peak current to 4.4μA; SGNF / SAM / Au electrode of peak current versus scan rate of the square root of The linear variance was R2 = 0.9968, with very good linearity. Description This structure of the electrode signal-to-noise ratio is high, cyclic voltammetry curves constituted mainly by the Faraday current. Linear fit slope of 10.638. Compared to the bare electrode, and a surface electrode having a nanostructure of a linear fitting larger slope, indicating that the effect is more sensitive to the frequency of this structure of the pair of electrodes electrochemical scanning rate.

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CLC: > Industrial Technology > Automation technology,computer technology > Automation technology and equipment > Automation components,parts > Transmitter ( converter),the sensor > Chemical sensors
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