Dissertation > Excellent graduate degree dissertation topics show

Fabrication of Electrochemical DNA Biosensors Based on Signal Amplified by Nanomaterials

Author: WangMingZhu
Tutor: ZhangYuZhong
School: Anhui Normal University
Course: Analytical Chemistry
Keywords: Electrochemical DNA Biosensor SWCNT-COOH Gold nanoparticles CuO nanospindles Co3O4 hexagonal nanosheets
CLC: TP212.3
Type: Master's thesis
Year: 2011
Downloads: 40
Quote: 0
Read: Download Dissertation

Abstract


Electrochemical DNA biosensor is an electrochemical DNA detection technology. As a result of this testing equipment with simplicity, low cost, high sensitivity, easy to use together with modern analytic technology. In some fields it has attracted much attention, such as environmental monitoring, clinical diagnosis and drug testing. The innovation of this paper is to fully utilize the unique characteristics of nano-materials and modern electrochemical technology to prepare several novel electrochemical DNA biosensors. This thesis includes four parts:Chapter I: Introduced the principle of electrochemical DNA biosensor, its research progress and application. Puting forward the idea of this paper.Chapter II: We report the fabrication of a sensitive electrochemical DNA impedance biosensor for the detection of sequence-specific target DNA. A single-stranded DNA probe with a NH2 group at the end （H2N–ssDNA） was then covalently immobilized on the surface of polymeric film at room temperature. The change of interfacial charge transfer resistance （RCT） was confirmed the DNA hybrid formation. The experiment result shows SWCNTs can significant amplify the impedance signal during the DNA hybridization detecting, and the DNA sensor has high sensitivity and high selectivity.Chapter III: We outline here the fabrication of a sensitive electrochemical DNA biosensor for the detection of sequence-specific target DNA. A single-stranded DNA probe with a thiol group at the end （HS-DNA） was covalently immobilized on the surface of the AuNPs through Au–S bond. Scanning electron microscopy （SEM） and electrochemical impedance spectroscopy （EIS） were used to investigate the film assembly process. Detection of DNA was performed by measuring the decline of peak current of the methylene blue （MB） by differential pulse voltammetry （DPV）. This DNA biosensor can detect the target DNA concentration in the range of 1.0×10-13 to 1.0×10-6 M, with a detection limit of 3.5×10-14 M （S/N = 3）. In addition, the DNA biosensor exhibits excellent selectivity for single-mismatched DNA detection.Chapter IV: We develop a sensitive electrochemical DNA biosensor based on gold nanoparticles/Co3O4 hexagonal nanosheets. Co3O4 hexagonal nanosheets were synthesized, and characterized by SEM and XRD. A single-stranded DNA probe with a thiol group at the end （HS-DNA） was covalently immobilized on the surface of the AuNPs through Au–S bond. Electrochemical impedance spectroscopy （EIS） was used to investigate the film assembly process. Detection of DNA was performed by measuring the decline of peak current of the methylene blue （MB） by differential pulse voltammetry （DPV）.

Related Dissertations

  1. A New Method for Thrombin Detection by Using Aptamers and Gold Nanoparticles,Q55
  2. The Process of Cysteine Self-assembly on Gold Nanoparticle Modified Electrodes and Their Application,TB383.1
  3. Application of Biofunctionalized Nanoparticles for Detection of Escherichia Coli O157:H7,R155
  4. Application of Allele-specific PCR in SNP Differentiation and Ferritin Nanoparticle Chemiluminescence Determination,TB383.1
  5. Synthesis and Application of Unilateral Divergent TEG-PAMAM Dendrimer,O631.3
  6. Studies on Competitive Mechanism Triggered Signal Amplification Based Aptasensors,Q503
  7. Fabrication of SERS-active Substrates and Their Utilization for Detection of Amino Acids and Bacteria Biomarkers,Q503
  8. Study of the Mechanism of DNA Polymerase Reaction and Detection of Single-base DNA Mutation with Fluorescence Resonance Energy Transfer Technique,Q523
  9. New multi- color fluorescent nano- biological materials and research,TB383.1
  10. Multifunctional Dendrimer-Entrapped Gold Nanoparticles: Synthesis, Characterization and Their Application in CT Imaging,O621.3
  11. Synthesis, Assembly and Catalytic Properties of Gold or Platinum Nanoparticle-doped Polymer Foam-like Thin Films at the Liquid/Liquid Interface,TB383.2
  12. Study of New Electrogenerated Chemiluminescence Method Based on Tris (2,2’-bipyridyl)Ruthenium(Ⅱ),O627.8
  13. The Application of Flow Injection Chemiluminescence and Its Combination of PLS in Pharmaceutical Analysis,O657.3
  14. Preparation and Application of DNA Biosensors Based on Gold and Magnetic Nanoparticles,TP212.3
  15. Preliminary Study on Rapid Determination of Bisphenol A by Electrochemical Method,O657.1
  16. Preparing Gold Nanoparticles Using Aspergillus Niger Spores as Templates and Its Catalytic Property,TB383.1
  17. Study on the Biosensor Based on Local Surface Plasmon Resonance (LSPR) of Gold Nanoparticles,TB383.1
  18. Kinetics of Trolox Nano-Antixoidants and Autoxidantion of Danshensu,R96
  19. Study on the Nanoparticles Probe Analysis by Chemiluminescence and Resonance Light Scattering Technique,O657.3
  20. Quantum dot -based electrochemical biosensor and the tumor markers detection,TP212.3

CLC: > Industrial Technology > Automation technology,computer technology > Automation technology and equipment > Automation components,parts > Transmitter ( converter),the sensor > Biological sensors,medical sensors
© 2012 www.DissertationTopic.Net  Mobile