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Screening of Oil-rich Microalgae and Its Oil for Biodiesel Production

Author: WangJinRong
Tutor: CaiLu; JiXiang
School: Inner Mongolia University of Science and Technology
Course: Genetics
Keywords: Navicula sp. nutrients algal oil biodiesel
CLC: Q949.93
Type: Master's thesis
Year: 2011
Downloads: 305
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With the increasing depletion of oil resources and the deterioration of ecologicalenvironment, every country in the world has been actively exploring environmentally-friendly alternatives to fossil fuels. Biodiesel, namely fatty acid methyl ester, is a cleanenvironmentally-friendly renewable energy resource. At present, biodiesel productionby microalgae is technically feasible, and it will be one of optimal solutions toachieving a perfect substitute for fossil fuels.In this work, a great amount of work of screening dominant microalgae specieswas conducted according to the climate and environmental characteristics of naturallakes of central and western regions in Inner Mongolia and one oil-rich strain ofNavicula sp. was obtained. Then the conditions of its growth and lipid accumulationwere optimized. Next algal cells were collected through the large-scale cultivation anddried to produce algal powder. Finally solvent extraction method was utilized to extractalgal oil for biodiesel production. Major research results are shown as follows:1. One oil-rich strain of Navicula sp. was successfully screened and algae speciespreservation methods were analyzed by comparison, one of which is able to make thealgae live to be9months and its growth improve faster when it is cultured again. Theimproved B&D oil extraction method has the ability to produce the maximum oil yieldfrom algal powder.2. Based on aquatic diatom medium, the optimal culture conditions for the growthof Navicula sp. were optimized as follows: temperature20℃, light intensity3000lx, pH8; the optimization of optimal concentrations of five kinds of nutrients was carried out byL16(45) orthogonal experiments and the results were obtained: urea50mg/L, K2HPO43H2O40mg/L, Na2SiO39H2O200mg/L, NaHCO310mg/L, MgSO47H2O70mg/L.Vitamin B1played significant role in promoting the multiplication of the algae, so itsoptimal concentration was200μg/L; Vitamin B12and vitamin H do not have evidentpropagation functions and the three vitamins don’t interact each other in the growth ofalgae. Navicula sp. went into the stationary phase on the11thday grown on theoptimized culture medium and biomass accumulation reached1.269atspectrophotometrically at680nm wavelength. 3. The results of stress of nutrients on lipid accumulation of Navicula sp. indicatethat nitrogen deficiency has significant role in lipid accumulation and the optimal ureaconcentration for oil accumulation was30mg/L, resulting in increase of comprehensivelipid content from30.60%to39.29%.4. Base catalysis transesterification method was utilized to convert algal oil intobiodiesel. Gas chromatography analysis demonstrated that main component of biodieselwas aliphatic acid containing C16and C18. Key performance indexes such as density,kinematic viscosity, flash point, acid value and iodine number were determinedaccording to U.S. standard ASTM6751-03and the Chinese standard GB/T20828-2007and it can be an ideal substitute for diesel.The utilization of algal oil of Navicula sp. for biodiesel production has uniqueadvantages that can alleviate the greenhouse effect, will not compete with grains andcereals consumed by mankind and takes up farmland on which crops are grown. This willdefinitely lay the foundation for large-scale biodiesel production from non-foodfeedstock and it has broad prospects in the future.

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CLC: > Biological Sciences > Botany > Botany ( Economic Botany ) > Grease,oil,wild plants
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