Dissertation > Excellent graduate degree dissertation topics show

The Application of Water Soluble Quantum Dots in Tumor Cell Lebeling and Establishment of Fluorescence Resonance Energy Transfer

Author: PuMingXing
Tutor: ShangQingKun
School: Northeast Normal University
Course: Analytical Chemistry
Keywords: CdTe/CdS core/shell QDs Fluorescence probes Cell labeling FRET
CLC: R318.51
Type: Master's thesis
Year: 2012
Downloads: 90
Quote: 0
Read: Download Dissertation

Abstract


In recent years, the application of water soluble quantum dot (QDs) in tumorcells imaging and fluorescence resonance energy transfer (FRET) have been widelyresearched. In this dissertation, different kinds of quantum dots, also with differentmaximum emission wavelengths are used in these two fields. The main contents andresults are as follows:1. CdTe/CdS core/shell quantum dots, which possessed the maximum emissionwavelengths at545nm and600nm, were synthesized by a simple aqueous solutionapproach based on CdTe. The as-prepared CdTe/CdS core/shell QDs with excellentoptical and fluorescence properties were characterized by UV-vis spectra,photoluminescence (PL), XRD and TEM. Two kinds of novle fluorescence probes,CdTe/CdS(545nm)-5-FU and bio-CdTe/CdS(600nm)-TAM, were prepared throughconjugating with anticancer drug5-fluorouracil (5-FU), P-gp inhibiting agentTamoxifen (TAM) and biotin respectively. The mechanism of the conjugations hasalso been investigated. The results of microscopic fluorescence image analysisindicated that bio-CdTe/CdS-TAM conjugates could target to the P-gp. Meanwhile,CdTe/CdS QDs loading the anticancer drug,5-fluorouracil (5-FU), were deliveredinto tumor cells without hindrance. This made the process of inhibition visualization,which will enlarge the application of QDs in imaging and therapy.2．CdTe quantum dots, which the maximum emission wavelengths were at530nm and610nm, were conjugated with two kinds of biomolecules, avidin andbiotin, respectively. The effects of reaction condition, such as pH value, reactiontemperature and reaction molar ratio were also investigated. We have successfullybuilded fluorescence resonance energy transfer (FRET) system using the strongaffinity and high reaction specificity between avidin and biotin. The effect of differentmolar ratios between donor and accepter to the FRET efficiency was researched. Thislaid the foundation for FRET technique applicated in pharmaceutical quantitativeanalysis. Meanwhile, the characterization of the conjugates and FRET system usingHPLC analysis method which is more rapid, accurate and reliable could significantlyincrease the analysis of accuracy and sensitivity.

Related Dissertations

  1. Novel Luminescent Nanomaterials Labeling Based Detection Method for Salmonella,TB383
  2. The Research on Fluorescence Spectrum Imaging and Photobleaching at Single Molecule Level,O657.3
  3. Studies on the in Vivo Interaction between MD-2 and TLR4 Based on FRET,R459.7
  4. Studies on the Endocytic Trafficking and furin Protease-induced Cleavage of Several Recombinant Pseudomonas Exotoxin Proteins in Hela Cells,Q93
  5. Fabrication and Properties Research of Novel Fluorescent Nanoparticles for Cupric Ion Detection,TB383.1
  6. Studies on the Synthesis of CD36 Transmembrane Peptides and Mechanism of Transmembrane Domain Interactions,R96
  7. Gold Nanoparticles Quenching Fluorescence Probe for Glucose Detection and Cell Imaging Based on Fluorescence Resonance Energy Transfer (fret),Q503
  8. Inverstigation of Sulfhydryl Oxidases and Their Probes,Q55
  9. Preparation of Fluorescent Quantum Dots Probes and Their Application in Detecting Staphylococcus Aureus,O471.1
  10. Preparation and Characterization of Ratiometric Mercury Ion Sensor Based on FRET,TQ316.334
  11. Preparation and biological analysis of semiconductor nanomaterials,TB383.1
  12. Quantitatively Imaging Induced Activation of Rac1, Cdc42 Signaling Pathways,Q26
  13. Effects of Cyclin D1 Over-expression on Activities of ClC-3 Chloride Channels,Q25
  14. Application of Fluorescence Resonance Energy Transfer (FRET) Technique in the Study of Cellular Signal Pathway,Q25
  15. Mechanism Study of the Biological Effects of Laser Irradiation in Living Cells,Q27
  16. Construction of a Novel Protease Biosensor Based on Fluorescence Resonance Transfer Technique,TH789
  17. Research on Mechemism of Photodynamic Threapy-Induced Apoptosis,R730.5
  18. Inducible Expression, Characterization of Mac-1-FP Fusion Protein in CHO Cells and Verification of Their Interaction by FRET,Q51
  19. Calcium Dynamics in Spine by FRET Based-on GFP,Q421
  20. Interaction between the N-terminal Domains of the AMPA Receptor Analyzed by Fluorescence Detection Methods,R33
  21. Identification of Interaction between Angiogenin and Progranulin,R73-3

CLC: > Medicine, health > Basic Medical > Medical science in general > Biomedical Engineering > Other branches of biomedical engineering > Light,laser biomedical
© 2012 www.DissertationTopic.Net  Mobile