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The Research of Embedding Pesticide Degrading Bacteria and the Performance Degradation

Author: ZuoJingJing
Tutor: FengGuiYing
School: Northwest University of Science and Technology
Course: Environmental Engineering
Keywords: Carbendazim Degrading bacteria Embedding optimization Degradation
CLC: X172
Type: Master's thesis
Year: 2011
Downloads: 37
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Abstract


Carbendazim is a broad spectrum fungicide, has been widely used in the prevention and treatment of diseases of various crops. The use of a large number of continuous carbendazim, so many plant pathogens developed resistance. Microbial remediation technology utilizes microbial metabolic capacity, the macromolecular organic pollutants into small molecule inorganic, no secondary pollution, easy to operate, low prices. But microorganism-low resistance to adverse environments, using the embedding method is conducive to shortening the processing time required to increase the microbial degradation. In this study, two carbendazim-degrading bacteria embedding conditions of the study and were further explored two degrading bacteria embedding physical properties and degradation conditions. The study concluded as follows: ⑴ Alcaligenes optimal conditions for embedding SA: SA concentration of 2%, CaCl 2 concentration of 4%, reaction time 24 hours; PVA embedding best conditions: PVA concentration of 10%, CaCl 2 3% concentration, reaction time 24 hours. ⑵ Rhodococcus optimal conditions for embedding SA: SA concentration of 3%, CaCl 2 concentration of 3%, reaction time 24 hours; PVA embedding optimal conditions for: PVA concentration of 10% , CaCl 2 concentration of 3%, reaction time 24 hours. ⑶ in embedding medium concentration, CaCl 2 concentration and reaction time of three factors, the reaction time is immobilized beads for removal of carbendazim most influential factor, followed by the embedding medium concentration, minimal impact is CaCl 2 concentration. ⑷ carbendazim degrading bacterium Alcaligenes optimum temperature is 30 ℃, the free bacteria carbendazim degradation rate 63.6%, SA package fungus ball carbendazim degradation rate of 81.5%, PVA bag fungus ball carbendazim degradation rate was 73.3%. The optimal reaction pH of 6, then the removal of free bacteria Carbendazim 52.5%, SA packet carbendazim bacteria removal of the ball 76.8%, PVA bag carbendazim bacteria removal of ball 78.9%. Determine the highest carbendazim 2:10 removal is the best inoculation. Under the optimum inoculum size, SA carbendazim removal of entrapped 75.8%; PVA embedding carbendazim removal was 78.1%. When carbendazim concentration of 100mg / L, the free carbendazim degradation rate of 91%, SA packet bacteria pellets and PVA bag fungus balls can carbendazim below this concentration completely degraded. Activation time is the best time 48h. Under these conditions, SA packet bacteria removal rate carbendazim ball 78.2%, PVA bag ball carbendazim bacteria removal was 80.3%. ⑸ red square red ball carbendazim degrading bacteria optimum temperature is 30 ℃, the free bacteria carbendazim degradation rate 62.7%, SA package fungus ball carbendazim degradation rate of 82.8%, PVA bag fungus ball carbendazim degradation rate of 87.1%. The optimum reaction pH of 6.5, then the removal of free bacteria Carbendazim 56.1%, SA packet carbendazim bacteria removal of the ball 76.7%, PVA bag carbendazim bacteria removal of ball 78.2%. Determine the highest carbendazim 2:10 removal is the best inoculation. Under the optimum inoculum size, SA carbendazim removal of entrapped 83.4%; PVA embedding carbendazim removal was 78%. When carbendazim concentration of 150mg / L, the free carbendazim degradation rate of 93.4%, SA packet bacteria ball to Carbendazim degradation rate 97.5%, PVA bag fungus ball to Carbendazim degradation rate of 98.2%. Activation time is the best time 48h. Under these conditions, SA packet bacteria removal rate carbendazim ball 80.2%, PVA bag ball carbendazim bacteria removal was 83.1%. ⑹ Alcaligenes carbendazim degrading bacteria with red square red ball carbendazim degrading bacteria are bacteria, external conditions for the two bacteria showed similar laws affecting the two embedding methods is also showing its effects similar variation.

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CLC: > Environmental science, safety science > The basic theory for the Environment and Science > Environmental Biology > Environmental Microbiology
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