Dissertation > Excellent graduate degree dissertation topics show

The Search of Potential Binding Sites of Serum Response Factor (SRF) and Research of the Profile of the Binding Sites of SRF in Human Genome

Author: LiZuoGang
Tutor: TaoShiZuo
School: Northwest University of Science and Technology
Course: Biophysical
Keywords: Serum response factor CArG-box To coprecipitation Technology based on microarray technology chromosome immune Position -specific scoring matrix Statistical analysis
CLC: Q75
Type: Master's thesis
Year: 2011
Downloads: 48
Quote: 0
Read: Download Dissertation

Abstract

Serum response factor (Serum Response Factor, SRF) is a highly conserved and widely exists in a variety of in vivo transcriptional regulator , it is about 10bp DNA segment sequence combination with about 1216 different lengths , also called CArG- box, and through this specific combination of time and space on the regulation of the expression level of target gene transcription . Serum response factor was first discovered in the proto-oncogene c-fos , which to some extent predict the degree of attention in the field of life science research , in particular, it is associated with a variety of research focus on the pathology , such as cancer , heart disease and so on. In the past, the majority of the serum response factor to the metabolic processes with a particular disease or life contact to expect answers to the role played by it in the course of their activities mechanism . However, for the cis-acting elements , particularly in a genomic scale , with different tissue expression linked rarely. This paper aims through the use of high-throughput data based on the microarray technology chromosome immune coprecipitation get to do a general description of its whole genome scale . And due to the Chip-on-chip data itself used the four cell lines as experimental materials, to a certain extent based experiments revealed that the transcriptional regulation of the difference between different cell lines may be provided. The experiment using ENSEMBL database of human genome-wide data GRCh37 as shear parent , to adopt the NHGRI initiated ENCODE plan in GSE8489 datasets Chip-on-chip data sets - locus data set . Use a single mutation consistency sequence , position -specific scoring matrix methods such as potential CArG-box search , and the results of the statistical analysis . The following conclusions: First , the same transcription factor in different organizations , are involved in the regulation of network and regulation of intensity is different ; , PSSM is used in the search of biological macromolecules sequence specific structure The more methods ; third, eukaryotes physiological and biochemical environment on the transcription of the gene encoding the existence of a significant effect ; fourth, under suitable physiological and biochemical conditions , SRF and a simple A / T sequence repeat binding ; fifth, in the different cell lines , SRF multimeric form different role .

The Search of Potential Binding Sites of Serum Response Factor (SRF) and Research of the Profile of the Binding Sites of SRF in Human Genome

Abstract

Related Dissertations