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Proteus widely distributed in nature, the normal flora of the human gut. Only left the the normal intestinal stranger into other parts of the body only cause infection. In hospitals, long-term broad-spectrum antibiotics, hormones, immunosuppressive agents, antineoplastic treatment decreased body resistance patients prone to infection, advanced examination and treatment methods to some extent also provides a way to invade the body Proteus Proteus infection plays an important role in the hospital. The phage treatment has been shown to many prominent advantages, especially for the use of antibiotics to treat animal bacterial infection prone to bacterial resistance and residual shortcomings. After a number of animal models and human clinical practice, the shortcomings and deficiencies in phage therapy, people have begun to select phage host range, role in the effectiveness of its role in the dynamic principle aspects of the transformation of the exploratory to obtain the desired therapeutic effect, to safeguard human and animal health. Experiment, we use double-layer agar plaque separation the purified Proteus phage, according to Molecular Cloning Guide Manual Version quarter-phage nucleic acid extraction method of extraction of nucleic acids and digested. Determination of the phage infection (MOI) to draw step growth curve. Analysis of the experimental results the following conclusions: In this study, 10 clinical isolates of Proteus host bacteria isolated from the residents of the sewage 4 toxicity phage, were named as phage Pr-v 01 phage Pr-V 02 phage Pr -v 03 04 phage Pr-v, which is formed plaques are circular, transparent, clear boundary. Phage Pr-v 04 titers of up to the 2.06 × 1012PFU/ml, with a diameter of 0.8 to 2.3mm lysis plaque. Cross experiments showed phage Pr-v 04 cracking ability, high titer, wide spectrum cleavage, the screening of the experimental study. Electron microscopy showed that phage PR-v 04 tadpole-shaped head was polyhedral three-dimensional symmetry, approximately 40 to 50 nm in diameter, with a short tail. Genomic DNA EcoR Ⅰ, Hind Ⅲ and Ned I digestion, proved to be a double-stranded DNA, its genome size is about 26kb, and more EcoR Ⅰ restriction sites. The phage Pr-v-step growth curve of 04 infected host incubation period of 15min role in the outbreak of the time about 18min, about 170 of the amount of cleavage. The phage Pr-v 04 infected its host Proteus vulgaris the best multiplicity of infection of 0.1. At 4 ℃, the plaque can be stored for about 10 months, and phage PR-v 04 with their host bacteria suspension can be stored for at least 6 months.
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