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Study on Improving Water-Solubility of Chitin Size by Enzyme

Author: ShenFang
Tutor: GuoJianSheng
School: Donghua University
Course: Textile Engineering
Keywords: Chitin Deacetylase Slurry Degree of deacetylation Water-soluble Sizing Properties
CLC: TS103.84
Type: Master's thesis
Year: 2010
Downloads: 35
Quote: 0
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Abstract


Slurry related to the quality and efficiency of the production of woven fabrics, but also related to the consumer and post-processing of the environmental and ecological. Yarn sizing process slurry ways to pollute the environment in two ways: First, the the slurry modulation and use the leftover pulp and untreated pulp-containing wastewater pollution emissions; weaving is completed, the fabric retreat the desizing wastewater generated in the pulp untreated emissions and pollution; another left in the fabric desizing scouring slurry composition, is not conducive to human health. So For Weaving enterprises, the sizing process using the green paste, sustainable development and environmental protection of the textile industry is of great significance. Chitin and its derivatives are recognized as a green polymer materials, can be used for sizing of chitin chitosan slurry. Slurry good film-forming properties, wear resistance, special fiber also has good adhesion and affinity. Good biodegradability and biological activity, but it also has excellent antibacterial and antimicrobial effect. This natural biological polymers in full compliance with environmental requirements, its excellent performance so that it remains to be developed for the new generation of excellent performance and perfect green slurry. But the chitin stable chemical structure limits its ability to dissolve only after processing into chitosan deacetylation, it only has a certain ability to dissolve. At present, most of Chitosan by chemical methods, broad molecular weight distribution of chitosan obtained by this method, uniformity, and a waste of energy, and environmental pollution. Using enzymatic, no side reaction, mild reaction conditions, the process is easier to control, is an ideal method, so the research and application of enzymes to improve the water-soluble chitin chitin slurry for improving the practical sex is important, and in line with the development trend of green paste. This research project with a specific enzyme that chitin deacetylase modified chitosan, on the basis of previous studies, to further improve the enzyme activity and the degree of deacetylation of chitin, and configure the slurry test the performance of the slurry and sizing performance. The first study on the production of chitin deacetylase strains i.e. slant culture of Aspergillus nidulans, and then through the liquid shake flask fermentation, the final extract supernatant by centrifugation to obtain chitin deacetylase. In order to improve the vitality of the chitin deacetylase studied parameters of culture conditions such as culture time, temperature, pH value, seed solution and inoculation amount of the enzyme activity, etc.. Through the experiment, the optimum conditions obtained fermented liquid culture: culture at 96h, the initial pH value of 6.5, fermentation temperature 31 ° C, 6% of the fresh amount of seed was inoculated, the production of chitin deacetylase enzyme activity basic stable 20-25U/mL. This topic is also chitin deacetylase at the the pretreated chitin substrate after deacetylation effect. In the liquid after the fermentation of the crude enzyme solution was centrifuged supernatant, and its role to have a certain degree of deacetylation of chitin, to study the impact of process parameters pH value, temperature and time on the effect of deacetylation, to the degree of deacetylation for test indicators, the use of orthogonal design experiments to identify the optimum conditions, the production and preparation of a 71.09% degree of deacetylation of chitosan products. The subject test a the chitin deacetylase modified after deacetylation degree of 60.75%, 63.18%, 57.42%, 33.33%, 47.11%, 54.05%, 50.44% water-soluble chitosan, the results show that when deacetylation degree of 54.05% and 57.42%, preferably water-soluble, both can be dissolved in an aqueous solution of a pH value of 6.5, reaches the neutral water-soluble, to meet the sizing requirements of the slurry pH. Subject is also a modified chitosan configuration into a slurry, test its slurry properties; arranged in accordance with a certain percentage of the mixed pulp, sizing experiments carried out on the yarn, test their sizing performance, and with the traditional slurry contrast. Comparative experiments, the chitin slurry of the performance of the enzyme-modified excellent has reached the sizing requirements.

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CLC: > Industrial Technology > Light industry,handicrafts > Textile industry,dyeing and finishing industry > General issues > Textile industry machinery and equipment > Textile accessories, equipment and auxiliary materials > Auxiliary materials
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