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OTL Detection for Ear-kernel Traits and Verification for Two100-grain Weight QTLs in Maize

Author: ZhouQiang
Tutor: LiYuLing
School: Henan Agricultural University
Course: Crop Genetics and Breeding
Keywords: BSA method QTL preliminary detection 100-grain weight QTLverification MAS
CLC: S513
Type: Master's thesis
Year: 2012
Downloads: 52
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Abstract


Maize yield is the result of coordinated growth for ear number per unitarea，grain number per ear and grain weight. The genetic researches ear-kernel traitshave guiding significance for yield increasing in maize. In this study，based on thephenotype analysis of ear-kernel traits for F1and F2populations from different normalcorn inbreds （with large grain size）×popcorn （with small grain size） crosses，the F2populations with extensive segregation were selected. Firstly，QTL of ear-kerneltraits was primarily detected by the bulk segregant analysis （BSA） method. Secondly，backcross populations constructed by MAS were used to verify and fine map the mainobjective QTL for grain weight. The main results in this study were as follows：1．The ear-kernel, plant and kernel nutritive traits were analyzed for the8inbredsand their F1and F2populations of12crosses between6normal corn and2popcorninbred lines. Both two kinds of inbreds had great influence for all traits in the F1andF2generations. The difference between the normal corn inbred line Scc and thepopcorn inbred line N10was great for all ear-kernel traits. And the segregation rangewas wide in the F2population. This population was selected to detect QTL ofear-kernel traits by BSA method.2．214SSR markers with polymorphism between parents Scc and N10wereselected by screening623SSR markers. Using BSA method, four SSR markers,phi001, umc2227, bnlg1866and umc2217, on chromosome1were found to be linkedwith100-grain weight, three SSR markers on chromosome5, umc1502, umc1941andumc1155was linked with100-grain weight QTL （qGW5）， umc1776on thechromosome2was linked with ear length. These three possible QTL were referred asqGW1, qGW5and qEL2, respectively.3．In order to prove the authenticity about the two100-grain weight QTLsdetected, two backcross populations （GW1-BC2F1and GW5-BC2F1） were constructedby MAS. These two populations had1680and1497plants, respectively. Each linkagemap of the two target regions was constructed using10SSR markers. qGW1wasmapped in the genetic region between umc1144and umc2390, and it explained10.16% of the total phenotypic variation. qGW5was mapped in the genetic region betweenumc1155and umc1502, and it explained9.10%of the total phenotypic variation.4．The genetic distance from qGW1to umc1144and umc2390was2.0cM and2.5cM. The genetic distance from qGW5to umc1155and umc1502was4.0cM and8.9cM. The two sets of bilateral markers could be used to MAS for qGW1and qGW5.By using MAS for qGW1and qGW5, the recovering ratio of genetic background ofthe plants selected from the BC3F1generation were94.92%and95.61%. This isbeneficial to construct the near-isogenic lines of the two target QTL.

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CLC: > Agricultural Sciences > Crop > Cereal crops > Corn ( maize )
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