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This thesis conducted Ginsenoside Rg1, Re, Rb1 matrix reference materials research, using high performance liquid chromatography (HPLC), capillary electrophoresis (HPCE) analysis of the principle of two different methods, Ginsenoside Rg1 , Re, Rb1 standard substance matrix value . Substance in accordance with the national standard technical specifications for the Ginsenoside Rg1, Re, Rb1 matrix reference material uniformity and stability tests investigated , the results show good uniformity of the standard substance , one year stability investigation results show that the standard substance meets the national standard substances on stability requirements . The standard material developed to fill the gaps in the domestic and international , in order to provide a method of analysis of American ginseng Confirmation basis , to ensure accuracy of the results from different laboratories , traceability , reliability and comparability . Established by ultra performance liquid chromatography - mass spectrometry Rapid Determination of ginseng ginsenosides Rg1, Re, Rb1 and ocotillol F11. Four kinds of saponins detection limits (S / N = 3) are no more than 12ng · g-1, the limit of quantification (S / N = 10) are no more than 40ng · g-1. The method has good precision and reproducibility of the four saponins recoveries were 94.0% ~ 105.6% (RSD = 4.53%), 91.9% -102.4% (RSD = 4.18%), 91.6% ~ 102.6% ( RSD = 4.57%), 95.0% ~ 102.4% (RSD = 3.03%). The method is established, American ginseng saponins study provides a more rapid and accurate detection and identification methods for quality and safety of commercially available ginseng provide a strong technical support.
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