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The Signal Transduction Mechanisms of Apoptosis Induced by Ginsenoside-Rh2 in HL-60 Cells

Author: MiaoZhiChun
Tutor: LuoZhiYong
School: Central South University
Course: Pathogen Biology
Keywords: Ginsenoside Rh2 Estrogen Receptor Tumor necrosis factor α Apoptosis Signal Transduction
CLC: R285
Type: Master's thesis
Year: 2010
Downloads: 28
Quote: 0
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Abstract


Ginsenoside Rh2 (ginsenoside-Rh2, G-Rh2) is one of the active ingredients of ginseng extract large number of domestic and foreign research confirmed that G-Rh2 has a strong activity in the prevention and treatment of cancer . In the previous study , our group found ginsenoside Rh2 can induce apoptosis in HL-60 cells , a dose - dependent manner , and the G-Rh2 treatment rationale HL-60 cells , tumor necrosis factor α (TNFa) were significantly increased . The estrogen receptor (estrogen receptor, ER) plays an important role in the cell 's life activities , has a complex impact on the development of the changes in the level of expression of estrogen receptors on tumor cells . G-Rh2 having estrogen-like effect, suggesting that the estrogen receptor with estrogen receptor binding and activation . This study as a model of human acute promyelocytic leukemia HL-60 cell line , significantly induced apoptosis in HL-60 cells , the optimal dose ( IC50 ) 25μM G - Rh2 with estrogen receptor antagonist role HL-60 cells . Use of flow cytometry to detect apoptosis changes ; technology using Western blotting analysis of experimental estrogen receptor of ERα of ERβ and tumor necrosis factor TNFα protein expression changes of ERα / ERβ-TNFα pathway mediated G-Rh2 - induced apoptosis in HL-60 cells . The results show that the G - Rh2 -induced upregulation of TNFα in the process of G-Rh2 -induced apoptosis in HL-60 cells , play a crucial role . G-Rh2 significant activation of the HL-60 cell TNFα protein expression , the expression of estrogen receptor p significantly increased apoptosis effects weaken after PHTPP to antagonize estrogen receptor p , suggesting that estrogen receptor p in G- plays an important role in the process of Rh2 -induced apoptosis in HL-60 cells .

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