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Thee Ffects of Chinese Herbal Monomeron Proliferation of NSC Through Modulating Caveolin-1/Notch Signal Pathway

Author: YuYue
Tutor: LiuBaiYan
School: Hunan University of Traditional Chinese Medicine
Course: Chinese medical science
Keywords: NSC(neural stem cell) neurogenesis icariin loganin Notch Caveolin
CLC: R285
Type: PhD thesis
Year: 2013
Downloads: 168
Quote: 0
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Abstract


Objective:To explore the effects of icariin and loganin on proliferation of NSC, study the molecular mechanisms of neurogenesis to provide proof for clinic.Methods:The NSC cultures were generated from the brain of embryonic mice. Primitive NSC were cultured, proliferated and passaged. The NSC were identified by the immunocytochemical staining of Nestin. BrdU was used to characterize the proliferation, and NF200, NSE, GFAP for the differentiation of NSC. Icariin and loganin on the proliferation of NSC were observed by the method of WST and neurospheres counting. The NSC from the brain of Caveolin-1knock-out mice were added to repeat the test of WST and neurospheres counting to investigate the mechanisms of Caveolin. The expression of Notch、 Hesl and Jagged1were detected by PCR.Result:The NSCs were successfully cultured and proliferated in vitro. Different dosages of Chinese Herbal Monomer could promote the proliferation of NSCs in vitro. We first investigated WST shows that except the group of loganin5×10-9mol/L、10-9mol/L (p>0.05), compared with the control group cell were increased obvious (p<0.05).The result of numbers of nerurospheres also corroborate the effect of the two Chinese Herbal Monomer could increased cell proliferation obvious (p<0.05), beside the group of icariin5×10-9mol/L and loganin5×10-mol/L and10-9mol/L (p>0.05).When we use the NSC from the brain of Caveolin-1knock-out mice to repeat the experiment. The WST shows that every group of icariin and loganin compared with the control group cell were increased obvious (p<0.05).The result of numbers of nerurospheres also corroborate with it.The result of PCR shows that icariin and loganin promote the proliferation of NSC in vitro significantly through up-regulated the expressions of Notch signal pathway, which the two different type of cells both manifestations the trend (p<0.05). From the comparison between the two kinds of cells were found, the deficiency of Caveolin-1could down-regulate the expressions of Notch signaling pathway between the same drug concentration group.Conclusion:Icariin and loganin promote the proliferation of NSC in vitro significantly through modulating Notch signal pathway expressions. Caveolin-1could regular the effect of Chinese Herbal Monomer on cell proliferation.

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