|
Aim: To observe the effects of polysaccharides from Dondrobium candidum (PDC)on mouse aging induced by D-galactose and investigate its possible mechanisms.Methods:90kunming mice were randomly divided into6groups (n=15), namely,control group, model group, JYL400mg/kg group, PDC40,80and160mg/kg groups.An aged mouse model was induced by subcutaneous injection of D-galactose120mg/kg when these mice were simultaneously treated with medicine by gavage. Theabilitiy of mouse learning and memory was examined after treatment with medicine for5weeks, the weight indexes of thymus and spleen, the activities of superoxidedismutase (SOD) and glutathione peroxidase (GSH-PX) as well as level ofmalondialdehyde (MDA) in serum, brain and liver were measured after treatment withmedicine for6weeks.Results: After treatment with PDC, the number of across original platform had anincrease trend, the time and distance of the fourth quadrant swim were significantlyincreased（p<0.05or p<0.01）, the latent period that mice first jumped down platformwas significantly delayed, and the number of electric shock was significantly decreasedas well（p<0.05or p<0.01）. On the other hand, the weight indexes of thymus andspleen, the SOD and GSH-PX activities in serum, brain and liver were significantlyincreased, while the MDA content in serum, brain and liver was significantly decreased（p<0.05or p<0.01）.Conclusion: PDC could improve the ability of learning and memory in aged miceinduced by D-galactose, which suggested that PDC possessed the antiaging effect, andits mechanisms might be related to enhancing the SOD and GSH-PX activities,subsequently scavenging the oxygen free radicals, and increaseing the immune function.
|