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Preliminary Study on the Mechanisms of Curcumol in Growth Effect in SGC7901Cells

Author: ChenHaiYan
Tutor: XuLiChun
School: Yangzhou University
Course: Traditional Chinese Medicine
Keywords: Curcumol proliferation apoptiosis MMP2 NO
CLC: R285.5
Type: Master's thesis
Year: 2012
Downloads: 11
Quote: 0
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Abstract


Objectives:To preliminary investigate the mechanisms of the effects of Curcumol in the growth effect on human gastric cancer SGC7901cells.Subject:Human gastric cancer SGC7901cellsMethods:Curcumol was dissolved in dehydrate alcohol and preliminary concentration of Curcumol was9mg/ml. Group1、2、3、4and5as experimental groups were set up. The Curcumol concentrations were set as follows:7.5μg/ml、15μg/ml、30μg/ml、600μg/ml、20μg/ml, control group contained1%dehydrate alcohol (dissolvent compare). After seeded for48h, the Trypan blue method was used to analyze the cytoactive of SGC7901; MTT assay was performed to study the inhibitory rate of SGC7901cells proliferation; the apoptosis rate were detected by FCM and the expression of Matrix metalloproteinase2(MMP2) were detected by Western Blot. Besides, after the effect of Curcumol on SGC7901cells for48h, the content of NO in cell culture medium were detected by NO kit.Results:1. The Trypan blue dyeing showed that the viable rate of SGC7901were more than90%, that fitted the requirement of experiment. The inhibitory effect of Curcumol on proliferation (P<0.05) in SGC7901cells were treated by the Curcumol concentration from7.5μg/ml to120μg/ml for48h were so obvious that comparing to control group, with the Curcumol concentration were increasing, the inhibition was gradually reinforced. Under the circumstance of30μg/ml Curcumol concentration, it produced the remarkable results of inhibiting tumor cells proliferation.2. The experimental groups of Curcumol concentration from7.5μg/ml to120μg/ml could induce apoptiosis of SGC7901cells after treated for48h detected by FCM assay. With the increase of Curcumol concentration, the apoptosis rate was improved. Comparing to the control group, it was significant (P<0.05). Under the circumstance of30μ g/ml Curcumol concentration, it produced the remarkable results of inducing tumor cells apoptiosis.3. The experimental groups of Curcumol concentration from7.5μg/ml to120μg/ml Curcumol could decrease the expression of MMP2in a dose dependent manner, comparing to the control group, it was significant (P<0.05)4. Comparing to the control group, the concentration from15μg/ml to120μg/ml Curcumol could regulate the content of NO in the culture medium,and with the concentration Curcumol added, the content of NO in the culture medium decrease, it was significant (P<0.05), but less than15μg/ml was not significant.Conclusion:1. The concentration groups from7.5μg/ml to120μg/ml could inhibit proliferation, and induce tumor cells apoptiosis. It initial showed that Curcumol resisted tumor celles growth effect through inhibiting proliferation and inducing tumor cells apoptiosis. In the process of experiment, we also discovered that under the circumstance of30μg/ml Curcumol concentration, it produce the remarkable results of inhibiting tumor cells proliferation and inducing the tumor cells apoptiosis, so the30μg/ml Curcumol concentration is the best Curcumol concentration to inhibit the tumor cells growing.2. Curcumol can restrain the expression of MMP2, besides, the Curcumol concentration groups from15μg/ml to120μg/ml could regulate the content of NO in the culture medium of SGC7901. It showed that Curcumol could inhibit the invasion and metastasis through restraing the expression of MMP2and reducing the content of NO in the culture medium.

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