Dissertation > Excellent graduate degree dissertation topics show

Experimental Study of the Four Traditional Chinese Medicine on the Proliferation of Rat Bone Marrow Mesenchymal Stem Cells into Chondrocytes

Author: ChenQiang
Tutor: CaiJianPing
School: Nanjing University of Traditional Chinese Medicine
Course: TCM orthopedics and traumatology
Keywords: Traditional Chinese medicine Induced differentiation Cartilage cell proliferation
CLC: R285.5
Type: Master's thesis
Year: 2012
Downloads: 97
Quote: 0
Read: Download Dissertation


OBJECTIVE:The observation of four Chinese medicine (Astragalus, Epimedium, snow lotus and Yougui drink) on the proliferation of rat bone marrow mesenchymal stem cells (BMSCs) to chondrogenic differentiation.METHODS:This study is divided rat bMSCs separation, culture, subculture and proliferation; induced to differentiate into cartilage-like cells and proliferation by transforming growth factor-β1(TGF-β1) in vitro observation of Astragalus, Epimedium, snow lotus and Yougui drink onby the proliferation of cartilage-like cells after induction of TGF-β1.RESULTS:Adherent method successfully isolated and cultured get rats bMSCs, cell morphology is a good, vigorous growth, cartilage-like cells the rat bMSCs induced by conditioned medium, immunohistochemical staining to observe the expression of collagen type Ⅱ, Astragalus, Epimedium, snow After the Lotus and Youguiyin the intervention, MTT assay were measured and statistical analysis showed that Astragalus Decoction can obviously promote cell proliferation, Youguiyin decoction of the four concentration groups have shown that inhibition of cell growth, Epimedium fried both agents to promote have inhibitory effect displayed worthless, the Saussurea alcohol extracts only10"5concentrations promote cell proliferation. The four Chinese medicines containing serum studies have shown that astragalus drug serum on cell proliferation in15%and1%concentrations of the of Epimedium drug serum concentration of15%and5%group display, the snow lotus drug serum10%,5%and1%three concentrations promote cell proliferation, Yougui drink the serum of four concentrations of drugs have shown to promote cell proliferation.CONCLUSION:Four kinds of traditional Chinese medicine to promote chondrogenic differentiation, the proliferation of only10-5snow lotus alcohol extraction liquid group, but also indicate that the four Chinese medicines containing serum has a role in promoting the proliferation of MSCs into cartilage differentiation.SIGNIFICANCE:Retrieve relevant documents, Yougui drink and the Astragalus drug decoction and snow lotus alcohol extracts to observe the cartilage-like cell proliferation induced by TGF-β1has not been reported by experimental studies, suggesting that four Chinese medicines containing serum on MSCschondrogenic differentiation, proliferation role in promoting new ideas into chondrocytes in the cartilage defect treatment.

Related Dissertations

  1. Expression of Prolyl Isomerase Pin1 in Osteosarcoma and the Effect of Regulation on Cell Cycle,R738.1
  2. Isolated systolic hypertension in patients with cardiac ultrasound characteristics of traditional Chinese medicine dialectical correlation exploration,R544.1
  3. Cloth and β- elemene combined administration of anti-tumor effect and mechanism of celecoxib,R96
  4. Professor Kong Guangyi academic thought Qing Xuan main Treating children cold cough,clinical research and clinical experience,R249.2
  5. Xu Peng age of academic thought and clinical experience medication regularity of the treatment of chronic gastritis,R249.2
  6. Proliferation Characteristics of Type 2 Porcine Circovirus Strains and Antivirus Activity of Iprs and Aps on PCV2 in Vitro,S858.28
  7. The Clinical Analysis between the TCM Syndromes of Subacute Thyroiditis (Hyperthyroidism Stage),R259
  8. Antitumor Effects of Elemene on the Proliferation Inhibition and Apoptosis Induction in Ascites Hepatoma Cell Line H22,R735.7
  9. Isolation and Cultivation of Rabbit Adipose-derived Stem Cells and the Influence of Platelet-rich Plasma on the Cell Proliferation,R329
  10. Primary Research on Strring Culture and Storing of Human Dermal Fibroblasts,R329
  11. Inhibitory Role of Plasmodium Yoelii Circumsporozoite Protein on the Proliferation and Survial of Tumor Cell,R730.5
  12. The Experimental Study of Semiconductor Laser Radiation and 5-FU Sustained Release Repressed Oral Malignant Tumor Cell,R739.8
  13. Study on the Cell Cycle of Liver Cells during Rat Liver Regeneration,Q253
  14. Effects of Different Immunization on the Development of Immune Organs,cell Proliferation and Expression of Apoptosis-relatived Gene in Chicken,S858.31
  15. Psoriasis vulgaris in different stages and card Hou T lymphocyte subsets,R275.9
  16. Effect of Bufalin on Proliferation and Induce Apoptosis of Vascular Endothelial Cells EAhy926,R285
  17. Effect of Esculentoside a on Apoptosis and Expression of PCNA、Caspase-3、Fas and FasL in Renal Tissue of BXSB Mice,R285.5
  18. Experimental Study of GUI QIN MISTURE on Adriamycin-induced Nephropathy in Rats,R285.5
  19. Down-regulation of ROCK Isoforms on Migration and Proliferation of Vascular Smooth Muscle Cells Stimulated by PDGF,R363
  20. Effects of Arsenic Trioxide on the Proliferation, Apoptosis and the Expression of BMP-2 Gene in Osteoblasts Cultured in Vitro,R363
  21. Maximum Tolerated Dose Combined with Metronomic Chemoterpy on Proliferation and Angiogenesis in Treatment of Breast Cancer,R737.9

CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology > Chinese medicine Experimental Pharmacology
© 2012 www.DissertationTopic.Net  Mobile