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Role of Heat Shock Protein-27Phospborylation in the Apoptosis of Human Umbilical Vein Endothelial Cells

Author: LiuShuJuan
Tutor: ChenGang
School: Fujian Medical
Course: Internal Medicine
Keywords: Heat shock protein-27 High glucose Endothelial cells Apoptosis PI3K/Akt ERK1/2
CLC: R363
Type: Master's thesis
Year: 2012
Downloads: 25
Quote: 0
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Abstract


OBJECTIVE: To investigate the role of heat shock protein-27(HSP-27)phosphorylation in the apoptosis of human umbilical vein endothelial cells(HUVECs) induced by high glucose. Further more, to explore the possiblemechanism and signal transduction pathways that regulate HSP-27phosphorylation and apoptosis of HUVECs induced by high glucose. METHODS:HUVECs were isolated from umbilical cords resulting from normal pregnancies,cells at passages2-3were used in this study. The activity of HSP-27wasevaluated by Western blot with specific phospho-HSP27antibody. Apoptosis ofHUVECs cultured with high glucose was analyzed by Annexin V-FITC/PIApoptosis Detection Kit. RESULTS: Compared with the control group, HSP-27phosphorylation of HUVECs cultured with30.5mmol/L high glucose for24、48and72hours increased100%(P<0.05),182.5%(P<0.01)and117%(P<0.05)respectively. Compared with high glucose group, the phosphorylationof HSP-27induced by high glucose for48hours was significantly blocked by theselective HSP-27inhibitor Quercetin(10umol/L), with the inhibition rate at52.6%(P<0.01). And the apoptosis of HUVECs increased37.6%by Quercetin.The phosphorylation of HSP-27induced by high glucose was blocked by thespecific phosphoinostide-3kinase (PI3K)inhibitor LY294002and the specificextracellular signal regulated kinase1/2(ERK1/2) inhibitor U0126in aconcentration-dependent manner, with a peak inhibition rate at62.6%and56.1%.The apoptosis rate of HUVECs in LY294002and U0126+high glucose group wasincreased50%,120.8%compared with high glucose group, respectively.CONCLUSION: HSP-27phosphorylation could be induced by high glucose, andblocked by Quercetin, had a more obvious endothelial cell apoptosis rates. HSP-27phosphorylation might be plays an protective role in mediating theapoptosis of HUVECs induced by high glucose. PI3K/Akt and ERK1/2areimportant pathways that contribute to the phosphorylation of HSP-27, apoptosis ofHUVECs in response to high glucose.

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