Dissertation > Excellent graduate degree dissertation topics show

The Drug Resistance Analysis and Epidemiology Characetristics of Shigella

Author: ZhouXue
Tutor: SunQiuLin
School: Anhui Medical University,
Course: Internal Medicine
Keywords: Shigella bacillary dysentery Integron drug-resistance PCR
CLC: R378.25
Type: Master's thesis
Year: 2013
Downloads: 9
Quote: 0
Read: Download Dissertation


ObjectiveTo vestigat the distribution and resistance of122clinical isolates of Shigella spp in32hospitals in Anhui Province,Sept.2011to guide clinical medication.To the investigate distribution of integron and gene cassettes embedded in122clinicalShigella isolates and analyze the multi-drug resistance of the integron positive Shigellaisolates.To the investigate epidemic characteristics and flora distribution of bacillary dysentery of Hefeiarea between2004and2011.Materials and MethodsThe122clinical isolates of Shigella spp. were collected in Sept.2011from32hospitals ofAnhui province. All isolates were cultured according to national guide to clinical laboratoryprocedures and were identified with the Microscan Walkaway-96System and serologicalidentification.Antimicrobial susceptibility testing was performed by agar dilution method. E. coli ATCC25922and ATCC35218were stored at the Anhui Center for Surveillance of BacterialResistance (Hefei, Anhui, China).PCR were carried out to detected IntI gene and gene cassettes in all Shigella isolates.Retrospective analyze the Shigella-detecting results of outpatient’s stool and the data of age,gender and other related information ResultsIn122clinical Shigella isolates,118isolates (96.7%) were S.flexneri,4isolates (3.3%) wereS.sonnei, no S.dysenteriae and S.boydii.The resistance rates of122isolates to piperacillin/tazobactam, cefmetazole and latamoxefwere lower than othercephalosporins. All isolates were susceptible to imipenem. And theresistance rates were highest to ampicillin and tetracycline.The resistance rates tociprofloxacin is28.69%, levofloxacin17.21%and, gatifloxacin13.11%.Most Shigella isolates were found to harbor one or two classes of integrons:91isolatespossessed a class1integron including11typical class1integron and60atypical class1integron,67isolates possessed a class2integron.The gene cassettes of typical class1integrons dfrA17-aadA5and dfrA12-orfF-aadA2were detected in22isolates. IntI2wasamplified in67Shigella isolates and all contained constant gene cassette arrays ofdfrA1-sat1-aadA1. After statistical analysising, the positive rate of class1integrons,atypicalclass1integron and class2integron was statistically significant wiht multi-drug resistant.There were248confirmed bacillary dysentery patients among10366suspicious outpatients.The detection rate was2.42%. The sex ratio approached one to one and the average age was25.24years old. Patients from0to40years old accounted for81.04%of all confirmed patients.Totally,there were114strains of S. flexneri,129strains of S. sonnei,4strains of S. boydii and1strain of S. dysenteriae.ConclusionsThe main serotypes of Shigella isolates in Anhui province2011were S flexneri, Shigellaisolates showed high resistance to several antimicrobials and multidrug resistance is fairlyserious. Cefoxitin, cefepime, ceftazidim, piperacillin/tazobactam, imipenem and levofloxacincan be used in treating patients infected with Shigella as empirical therapy in our region. Theexistence of integrons plays an important role in drug resistance. Young adults are vulnerable to acute bacillary dysentery, and the gender difference is minimal. S. sonnei has surpassed S.flexneri in incidence rate and become the most common cause of bacillary dysentery.

Related Dissertations

  1. Cloning and Expression of CHS and CHI Genes and Their Regulation on the Accumulation of Flavonoids in ’Cara Cara’ Navel Orange (Citrus Sinensis Osbeck) and ’Guoqing NO.4’ Satsuma Mandarin (Citrus Unshiu Marcow),S666.4
  2. Control of Verticillium Wilt Disease of Cotton Plants with the Application of a Bio-Organic Fertilizer and Its Microbiologecal Mechanism in Rhizosphere,S144.1
  3. Effect of Organic Nutritiveadditives on Soil Micro-ecological Restoration and Its Mechanism.,S143
  4. Study on Key Technology and Its Physiological Basis of Virus Elimination and Rapid Propagation of Phalaenopsis,S682.31
  5. Invasion of Human Shigella to Chicken and Intestinal Epithelial Cell and the Expression of CD44 in Developing Chicken Intestine,S855.1
  6. Proliferation Characteristics of Type 2 Porcine Circovirus Strains and Antivirus Activity of Iprs and Aps on PCV2 in Vitro,S858.28
  7. The Dynamic Distribution of Shigella Isolated from Chicken in Infected Chickens,S858.31
  8. Molecular Epidemiological Analysis of Duck Hepatitis B Virus in Cherry Valley Duck in Henan Province and Anti-duck Hepatitis B Virus Activities of FNC in Vivo,S858.32
  9. Proliferation and Differentiation Effects of Different Culture Methods and Cell Factors on Mouse Spermatogenic Cells in Vitro,R329
  10. Distribution and Identification of Cereal Cyst Nematode on Wheat in Jiangsu Province and Their Chemical Control,S435.121
  11. The Impact of Histone Deacetylase Inhibitor Trichostatin a on Proliferation and Apoptosis of Porcine Granulosa Cells,S828
  12. The Dynamical Evaluation of Three Methods to Diagnose Mycoplasma Pneumoniae Infection in Children,R725.6
  13. Virulence Factors of Enterococcus Faecalis Expression in the Biofilm of Starvation Phase and after Two Root Canal Conventional Drugs,R780.2
  14. Construction of an Full-Lengh Genomic cDNA Clone of Hev SAAS-JdY5,R346
  15. Experimental Studies on the Different Expression of Myosin Heavy Chain in Rabbit Semimembranosus’ Sub-department,R322
  16. Eualuation of Acute Toxicity of Liu-She-Wan in Mice and Its Antitumor Effects in Hep3B Cells,R285.5
  17. Study on Coencapsulation of Bone Marrow Mesenchymal Stem Cells and Somatic Cells,R329
  18. The Expression and Clinical Significance of the Family of Apoptosis Stimulating Protein of P53 in Gastric Cancer,R735.2
  19. Studies on the Quality of Phisio-Chemical and Fresh Keeping Technologies of Post-Harvest in Volvariella Volvacea,S646.13
  20. Application of SNP Analysis by Pfu High-fidelity DNA Polymerase with Double Phosphorothioate Modified Allele Specific Primers in Direct Genotyping from Whole Blood,R91
  21. Lesion Bacterial Investigation of Patients Suffer from Cutaneous Squamous Cell Carcinoma,R739.5

CLC: > Medicine, health > Basic Medical > Medical Microbiology ( pathogenic bacteriology,pathogenic microbiology ) > Pathogenic bacteria > Enteric bacilli > Shigella genus ( Escherichia Shiga (Shiga) )
© 2012 www.DissertationTopic.Net  Mobile