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Protective Effect of Lipo-prostaglandin E1on Renal Damage Induced by Experimental Severe Acute Pancreatitis

Author: LiuChaoXiang
Tutor: LuoYuHong
School: Jinan University
Course: Surgery
Keywords: Lipo-prostaglandin E1 severe acute pancreatitis kidney damage ThromboxaneA2 tumornecrosis factor-α
CLC: R657.51
Type: Master's thesis
Year: 2012
Downloads: 27
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Abstract


ObjectivesTo evaluate the protective effect of Lipo-prostaglandin E1against kidney injury induced bysevere acute pancreatitis in rats and to investigate its mechanism by observing the dynamicchanges of PGI2,TXA2,TXA2/PGI2and TNF-α in serum.MethodsA total of90healthy Sprague-Dawley (SD) rats were randomly divided into five groups:the sham operation group (SO), severe acute pancreatitis group (SAP group) and Lipo-PGE1treatment of low-dose group (PG1group), medium dose group(PG2group), high dose group(PG3group), each group18. SAP was induced by retrograde injection of3.5%sodiumtaurocholate into bile duct at dose of1ml/kg of the body weight. On the basis of the SAP modelgroup, Lipo-PG E1low, medium and high dose groups was intraperitoneally injected inrespective doses of5μg/kg,10μg/kg,20μg/kg; SO group was injected with the same volume ofsodium chloride solution after being flipped the duodenum and pancreas gently, each groupwere hypodermically injected saline solution in dose of30ml/kg after operation.The rats ineach group would be opened again for observation, and sampled for testing12-24hours afterthe operation. Testing indicators:1. serum amylase(AMY);2.serum creatinine(SCR);3. serumurea nitrogen(BUN);4.serum prostaglandin I2(PGI2);5. serum thromboxane A2(TXA2)6.serumtumor necrosis factor-α (TNF-α);7. Pancreatic biopsy and pathological score;8.Kidney biopsyand pathological score. All data would be handled by SPSS13.0statistical software.ResultsThere were no significant changes of all parameters at all time point in the SO group(P>0.05). Compared with the SO group: SAP group at both time points, the level of serumAMY,SCR,BUN,PGI2,TXA2and TXA2/PGI2and the pathology score of pancreas and kidneywas significantly increased, the difference was statistically significant (P<0.05), the group of all time values was no statistically significant (P>0.05). Compared with SAP group, the level ofAMY, SCR, BUN and the pathology score of pancreas and kidney in PG1group wassignificantly decreased (P<0.05), especially the level of AMY decreased more significantly overtime, but the level of PGI2, TXA2,TXA2/PGI2and TNF-α decreased not significantly (P>0.05);in addition to PGI2, TXA2in PG2group,other indicators were decreased (P<0.05), there was nosignificant difference in two points time (P>0.05); every indicator of PG3group decreasedsignificantly (P<0.05), without significant difference in two points time (P>0.05); the treatmentof PG3group was significantly (P<0.05).Conclusions1,the model of SAP can be formed quickly after injecting into rats’ bile duct with3.5%Sodium taurocholate;2, TNF-α, PGI2and TXA2are key substances that response toinflammatory in SAP, the concentration disorder of them cause SAP-related kidney damage;3,Lipo-PG E1can reduce renal damage in the SAP,the mechanism may be related with thedecrease of TNF-α on one hand, and the improvement of the microcirculation by TXA2/PGI2on the other hand;4, among three groups of5μg/kg,10μg/kg and20μg/kg, the treatment of20μg/kg group are the most significant.

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CLC: > Medicine, health > Surgery > Of surgery > Abdominal surgery > Pancreas > Pancreatitis
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