Dissertation > Excellent graduate degree dissertation topics show

The Local Arterial Infusion of Rabbit-anti-rat Thymocyte Globulin in a Rat Model of Limb Allotransplantation: an Experimental Research

Author: LiLiang
Tutor: PeiGuoXian; BiLong
School: Fourth Military Medical University
Course: Surgery
Keywords: Limb allotransplantation Local arterial infusion Rabbit-anti-rat thymocyte globulin rat
CLC: R658.3
Type: Master's thesis
Year: 2013
Downloads: 1
Quote: 0
Read: Download Dissertation

Abstract


Objective:In order to investigate the impact of different ways of administration ofrabbit-anti-rat thymocyte globulin (rATG) on the systemic state of recipient and thesurvival of allograft in a rat hind limb allotransplantation model. Find a new method oftreatment which has less impact on the general state and better immunosuppressiveeffect.Method: In situ autologous limb transplantation was conducted in6inbred Lewis (LEW,RT11) rats, which were regarded as the autotransplantation control group (group A).Then limb allptransplantation models were built using inbred Lewis rats (n=24) as therecipient and inbred Brown Norway (BN, RT1n)(n=12) as the donor. They wererandomly divided into4groups according to the different administration method ofrATG, including the allotransplantation control group (group B, no treatment after thetransplantation, n=6), the intraperitoneal injection group (group C, intraperitonealinjection of rATG,1mg/kg/d, q.d., n=6), the intravenous infusion group (group D,sustained infusion of rATG through femoral vein,1mg/kg/d, n=6) and the arterialinfusion group (group E, sustained infusion of rATG through femoral artery,1mg/kg/d,n=6). The general situation of recipients and the appearance of allografts wereobserved periodically after the transplantation operation, and the mean survival time ofallografts in each group was recorded. At the sixth day post operation, the tissuespecimen was taken from each group and observed under the optical microscope, themean rejection grade (MRG) score of each group was measured according to thedegree of rejection. At the day of-1,1,3,5,7,9,11post operation, serum of eachgroup was collected, and the concentration of IL-2was detected using enzyme-linkedimmune sorbent assay (ELISA).Results:The general situation of group A and group E was well, demonstrating a rapidlyincreasing body weight. Other groups demonstrated a slowly increasing body weightand suspected poisonous side effect. Group A showed an indefinite survival ofautografts. The mean survival time of group B, C, D, E was6.7±0.8、7.8±0.8、8.5±1.0and16.7±1.2days, respectively. There was a significant difference between each group(F=131.520, P<0.01). The survival time of group E was significantly longer than theother groups (P<0.01). The MRG score of group B, C, D, E at sixth day post operation was2.8±0.2、2.5±0.2、2.3±0.2and0.5±0.3, respectively. There was asignificant difference between each group (F=140.335, P<0.01). The MRG score ofgroup E was significantly lower than the other groups (P<0.01). The IL-2concentration in the serum of group B, C, D and E increased along with the passage oftime. The ratio of increase arranged in descending order: group B, group C, group Dand group E. The IL-2concentration in the serum of group E was significantly lowerthan group B, C and D (P<0.05) at each time spot except the-1and1day postoperation.Conclusion:Compared with the method of intraperitoneal injection and intravenous infusion,arterial infusion of rATG can suppress the acute rejection of limb allograft moreeffectively with less impact on the general situation of recipient. Local arterial infusionof rATG may be more applicable to the immunosuppressive therapy after limballotransplantation.

Related Dissertations

  1. The Effect of Tanshinone ⅡA and Niacin on the Cardiovascular Function of Infant Obese Rat,R285.5
  2. Study on the Cell Cycle of Liver Cells during Rat Liver Regeneration,Q253
  3. The Regulatory Role of Proliferation and Apoptosis of ERK1/2 and JNK Signaling Pathways in Eight Liver Cell Types during Rat Liver Regeneration,Q418
  4. BMP2 and VEGF165 Genes Transfected Rat Bone Marrow Mesechymal Stem Cells Simultaneity Expression,R329
  5. Effects of High-salt Dieet on Blood Pressure and the Remodeling of Carotid Arteries and the Intervention of Telmisartan in Wistar Rats,R544.1
  6. The Espression of C-reactive Protein in Rats of the Chronic Obstructive Pulmonary Disease with Diabetic,R563.9
  7. Relationship and Significance of Gene Transcription Profiles in Rat Liver Regeneration and Non-Alcoholic Fatty Liver Disease Occurrence,R575.5
  8. The Expression of Visfatin in Liver of Rats in Different Glucose Metabolic Statuses and Effects to Glucose and Energy Metabolism,R587.1
  9. Morphological Characteristics of Brain Capillary Endothelial Cells and Neurons in a Ticellular System for Study on Neuroprotection and Neuroregeneration in Vitro,R651.1
  10. Influences of Temperature on SCF/c-kit Prolifertation Pathway and Cell Cycle of Rat Spermatogonial Cells Cultured in Vitro,R698
  11. Rat liver regeneration and acute liver failure occurring gene transcriptional profiling studies and their significance,Q418
  12. Osthol on bleomycin- induced pulmonary fibrosis in rats and its mechanism of,R563.9
  13. Improving the Voding Disfunction by a 5-HT2A Receptor Agonist in Rats with Chronic Spinal Cord Injury,R651.2
  14. Expression of MMPs and the Effects of the Intervention of Calcitonin in Rat Osteoarthritis Cartilage,R684.3
  15. Statistical Inference for Spatial Distribution of Rat Density in Three Gorges Reservoir Before and After Water Impounded,R184
  16. Experimental Study on the Effect and Mechanism of Bone Marrow Mesenchymal Stem Cells Treating the Rats β-irradiated Skin Injury,R641
  17. An Experimental Study on the Protective Effect of Edaravone on Liver Graft against in Rat OLT Model,R657.3
  18. Oridonin preconditioning on rat hepatic resection autologous transplantation ischemia -reperfusion injury in rats and its mechanism,R657.3
  19. Intervention of Common Turmeric N-Butanol Extraction on Pancreas Fibrosis of Rats,R285.5
  20. The Effect of Different AUR Duratin on the Function and Structure of Kidney in Rats,R694.55
  21. Experimental Study of the Effects of Butylphthalide on the Survival of Long Random Pattern Skin Flap in Rats,R622

CLC: > Medicine, health > Surgery > Of surgery > Limbs outside the science > Lower limb
© 2012 www.DissertationTopic.Net  Mobile