Dissertation > Excellent graduate degree dissertation topics show

Proteomics Study of Cerebrospinal Fluid in Multiple Sclerosis Patients

Author: SunZuo
Tutor: YangZuo
School: Jilin University
Course: Neurology
Keywords: Multiple Sclerosis (MS) Cerebrospinal fluid (CSF) Proteomic Fibulin-1(FBLN-1) Nidogen-2(NID-2) Apolipoprotein A-I (ApoA-I) Apolipoprotein E (ApoE) Amyloidprecursor protein (APP) Clusterin (CLU) Transthyretin (TTR)
CLC: R744.51
Type: Master's thesis
Year: 2013
Downloads: 45
Quote: 0
Read: Download Dissertation


Multiple sclerosis (MS) is a common autoimmune disease with the feature of centralnervous system demyelination. The clinical manifestations of MS are recurrent attacks andrelapsing-remitting. The pathological characteristics include inflammatory infiltrationsurrounding the small vessels, demyelination of myelin sheath, astrocyte proliferation,oligodendrocyte and axon damage. However, the etiology and pathogenesis of MS is knownunclearly. In order to explore the underlying molecular mechanism of MS, specificbiomarker protein related with MS development should be identified firstly. Then, themechanism of MS development and self-remyelination may be studied.Objective: Proteomics analysis was used to inquire differential proteins in CSF of MSpatients in order to detect the disease-specific proteins (DSPs). The purpose of this study isto provide new research evidences for MS clinical diagnosis, treatment, prognosisimprovement, and detectetion of drug targets.Methods:15patients were divided into2groups, MS group and Control group.Two-dimensional gel electrophoresis (2D-DIGE) combined with matrix-assisted laserdesorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) were used toidentify DSPs in MS patients’ CSF.Results: Compared with Control group,7differtial proteins were identified in MSgroup.(1) Apolipoprotein A-I (ApoA-I) expression up-regulated obviously in1.83timeswith significant statistical difference (P<0.005);(2) Apolipoprotein E (ApoE) expressiondown-regulated obviously in2.37times with significant statistical difference (P<0.005);(3)Beta amyloid precursor protein (β-APP) expression down-regulated obviously in1.91times with significant statistical difference (P<0.005);(4) Clusterin (CLU) expressiondown-regulated obviously in2.16times with significant statistical difference (P<0.005);(5)Transthyretin (TTR) expression down-regulated obviously in1.79times with significantstatistical difference (P<0.005);(6) Fibulin-1(FBLN-1) expression down-regulatedobviously in1.5times with significant statistical difference (P<0.005);(7) Nidogen-2 (NID-2) expression down-regulated obviously in1.72times with significant statisticaldifference (P<0.005).Conclusion:1.7DSPs were identified in MS group, including up-regulated ApoA-Iand down-regulated ApoE, CLU, β-APP&FBLN-1. These DSPs were supposed toparticipant in MS pathogenesis through specific mechanisms.2. It is the first time thatFBLN-1and NID-2was reported down-regulating in CSF of MS patients.Their definitefunctions are unknown and need further studies.

Related Dissertations

  1. Analysis of Serum Biomarkers Screening for Diagnosis of Stage Ⅰ Gastric Cancer Using SELDI-TOF-MS,R735.2
  2. Differential Proteomic Analysis of Two Brassica Napus Lines Differing in the Oil Contents,S565.4
  3. Deinococcus radiodurans gene DR1709 mutant proteome analysis,Q936
  4. Comparative Related-antigens and Proteomic Analysis of the Pathogenic Yersinia Enterocolitica Bio-serotype 1B/O:8 and 2/O:9 Cultured at Different Temperatures,R574
  5. Comparison and analysis of deltamethrin resistant strain and susceptible strains of Plutella xylostella proteome,S433
  6. Removal of high-abundance proteins in serum proteomics of lung cancer after HPLC separation and spectrum analysis,R734.2
  7. Application of the Label-free Quantitative Proteomics Technical Analysis Gastric Cancer Differentially Expressed Proteins,R735.2
  8. Proteomic Analysis of Bacillus Thuringiensis Strain 4.0718 at Different Growth Phases,S476
  9. The Protective Effect of Apolipoprotein A-I on Ox-LDL-induced Human Arterial Wall Injury,R543
  10. Preliminary Study on Biodegradation of Benzo[a]pyrene by Marine Microorganisms,X172
  11. Observation of EMA and CEA in Meningeal Carcinomatosis Cells of Cerebrospinal Fluid by Laser Scanning Confocal Microscopy,R739.45
  12. Serum protein profiling combined with artificial neural network diagnosis of esophageal cancer pathological differentiation model of applied research,R735.1
  13. A Study of the Therapeutic Effects of Apoliprotein A-I on Endotoxemia in Mice Induced by Lipopolysaccharide,R631
  14. Proteomics Analysis of Porcine Alveolar Macrophages Infected with Porcine Circovirus Type 2,S858.28
  15. The Correlation Study of Proteomics and Psychological Foundation of Anxiety Disorders,R749.7
  16. Study on the Content Changes of Neuron Specific Enolase in the Patients with Hemorrhagic Stroke,R743.3
  17. Effects of Ultraviolet Light on the Secondary Metabolism of Clematis Terniflora DC. and Related Proteomic Study,R341
  18. Proteomic Identification of Differentially Expressed Proteins in Arabidopsis Rosette Leaves in Response to Exogenous Methyl Jasmonate,Q946.1
  19. Study on the PAHs Degradative Characteristics and the Metabolic Pathway of Sphingomonas Sp. H,X172
  20. Studies on Species-specific and Physiological Biomarkers of Key Harmful Algal Bloom Species from the Coast of China Sea,X55
  21. Analysis on Differential Proteomic Fingerprints for Advanced Gastric Cancer Patients with LGT Fingerprints Changing,R735.2

CLC: > Medicine, health > Neurology and psychiatry > Neurology > Spinal cord disease > Demyelinating disease > Multiple sclerosis
© 2012 www.DissertationTopic.Net  Mobile