Dissertation > Excellent graduate degree dissertation topics show

Expression and Implication of Activating Transcription Factor5in Colorectal Adenocarcinoma

Author: SunXiaoWei
Tutor: ZhangJianLi
School: Qingdao University
Course: Surgery
Keywords: Colorectal Neoplasms Activating Transcription Factors Bcl-2 Apoptosis
CLC: R735.3
Type: Master's thesis
Year: 2012
Downloads: 6
Quote: 0
Read: Download Dissertation


AIM:To investigate the expression of ATF5(Activating Transcription Factor5) and Bcl-2(B-cell lymphoma/Leukemia-2) in colorectal adenocarcinoma.Methods:RT-PCR and S-P staining was used to examine the mRNA and protein expression level of ATF5and Bcl-2in56colorectal adenocarcinoma and adjacent tissues.Result:The expression levels of both ATF5and Bcl-2mRNA in colorectal adenocarcinoma tissues were higher than the adjacent tissues(P<0.01). And they were related to the differentiation degree and Dukes clinical stagermore ATF5mRNA was detected in poorly differentiated adenocarcinoma tissues than well/moderately differentiated adenocarcinoma tissues(P<0.05) and more in Dukes stage C-D than stage A-B(P<0.05):conversely,less Bcl-2mRNA in poorly differentiated adenocarcinoma tissues than well/moderately differentiated ones(P<0.05) and less in Dukes stage C-D than stage A-B(P <0.05).The immunohistochemistry study indicated that most of the adenocarcinoma tissues(45/56) showed cytoplasm immunoreactivity for ATF5while only a few cells located at the basal part of the lacune showed ATF5staining in adjacent tissues(8/56).Similarly,more colorectal adenocarcinoma tissues (30/56)showed Bcl-2staining than adjacent(5/56). The protein expression levels of both ATF5and Bcl-2were related to the differentiation degree and Dukes clinical stage too,just like the result of RT-PCR.Conclusion:The abnormal expression of ATF5may play an important role in the development and progression of colorectal adenocarcinoma. As it was related to the differentiation and Dukes clinical stage,it can be a new index to predict the prognosis and targeting ATF5may be a potential selective approach for colorectal adenocarcinoma therapy.ATF5may play it’s role by regulating the expression of Bcl-2in colorectal adenocarcinoma.

Related Dissertations

  1. The Study on Cryopreservation and Mechanism of Freezing Injury on the Spermatozoa of Coelomactra Antiquate,S968.3
  2. The Anti-tumor Effect of CADPE and Inducing Apoptosis in Human Gastric Cancer Cells,R735.2
  3. Cloth and β- elemene combined administration of anti-tumor effect and mechanism of celecoxib,R96
  4. TRAIL in the regulation of tumor invasion CD4 ~ CD25 ~ Treg,R730.2
  5. The Study of Lead Exposure on Cyprinus Carpio Ovary Epithelial Cells,X174
  6. Effects of Diclazuril on G3PDH in Second-generation Merozoites of Eimeria Tenella,S858.31
  7. The Effects and Preliminary Study of PCV2 on Ca2+ Signal in Lymphocytes of Piglets,S858.28
  8. Effects of Angiogenic Factor on Follicular Angiogenesis and Development in Sexual Maturity Mice,S852.2
  9. Expression of β-Catenin in Pig’s Ovary and the Effect of β-Catenin on Porcine Granulosa Cells Apotosis and Steroidogenesis Related Enzyme,S828
  10. Induced Combination Apoptosis by FB1 and AFB1 in Vero Cell,S856.9
  11. The Apoptosis Mechanism Induced by Aflatoxin B1 and Deoxynivalenol in Primary Hepatocyte of Cyprinus Carpio,S856.9
  12. Effects of BMPR-IB Gene Silencing by Small Interfering RNA on Apoptosis of Porcine Pollicular Granulosa Cells and Ecpression of BMP Pathy-Way-Ralted Genes,S828
  13. The Effects of Don on Proliferation, Differentiation, and Apoptosis of Chondrocytes in Chicken,S858.31
  14. The Effect of Foxol on Apoptosis of Mouse Granule Cells,S865.13
  15. Study of Melittin on Tumor Inhibition and Part of Mechanism of Human Hepatocellular Carcinoma HepG-2 Xenograft in Nude Mice,R735.7
  16. The Protective Effect of Rat Myocardial Ischemia/reperfusion Injury Following Bone Marrow Mesenchymal Stem Cell Pretransplantation for 1 Week,R542.22
  17. Expression of WW Domain Containing Oxidoreductase Gene in Cholangiocarcinoma and Its Effect on the Biological Behavior of Cancer Cell Line QBC939,R735.8
  18. The Effects of (180F-FDG on the Apoptosis of Eca-109 Esophageal Cancer Cell Line,R735.1
  19. The Signal Mechanism Underlying DON Induced Apoptosis in BHK-21 Cells,S856.9
  20. The Expression and Mechanism of NF-κ B and IL-6 after 90% Portal Vein Ligation in Rats,R657.3
  21. The Study of Mitochondrial Signal Mechanism Induced Apoptosis by Don in Human Colon Cancer Cells,R735.35

CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Intestinal neoplasms
© 2012 www.DissertationTopic.Net  Mobile