Dissertation > Excellent graduate degree dissertation topics show

The Mechanism of MtDNA Cause of SIRS and on the Preliminary Study of Protection Effect to the the SIRS of Chloroquine and PDTC

Author: ZhouMeng
Tutor: SunTianSheng
School: Anhui Medical University,
Course: Surgery
Keywords: mtDNA SIRS chloroquine TLR9 NF-κB
CLC: R459.7
Type: Master's thesis
Year: 2012
Downloads: 38
Quote: 0
Read: Download Dissertation

Abstract


Objective The body is caused to a systemic inflammatory response syndrome, or evenmultiple organ failure by the trauma. The pathogenesis and prevention of the systemicinflammatory response syndrome has not yet completely understood. It is well knownthat the body can produce a large number of mtDNA after trauma, mtDNA can beactivated P38protein kinase pathway to produce inflammatory factors, leading to asystemic inflammatory syndrome, but the mtDNA whether through TLR-9activation ofNF-κB pathway and inflammation factor is still unknown. Using in vivo tests, the firstinjection of different concentrations of mtDNA, the level of detection in peripheralblood of various inflammatory factors change, observed lung inflammatory infiltration,to explore the mechanism of mtDNA lead to SIRS; blockers chloroquine and PDTC onthe basis of this preliminary understanding the protective effect of chloroquine andPDTC for mtDNA cause of SIRS lower of SIRS, prevention and treatment ofmulti-organ damage and complications related to new ideas for the treatment of severetrauma.Methods The first part of the rats were divided into8groups;①buffer group (n=2)the②nDNA and (10ug/ml) group (n=14)③of mtDNA (15ug/ml) group (n=14)(4)mitochondrial fragments (final concentration containingmtDNA1μg/ml, MTD1) group(n=14)⑤of mtDNA (5ug/ml) group (n=14)(6) mitochondrial fragments (finalconcentration is containing mtDNA5μg/ml, MTD5) group (n=14)⑦of mtDNA(15ug/ml) group(n=14), the⑧mitochondrial fragments (final concentrationcontaining mtDNA15μg/ml, MTD15) group (n=14). The second part of the rats were divided into four groups:①of mtDNA (15ug/ml)+chloroquine (10mg/kg) group (n=14)②of mtDNA (15ug/ml)+chloroquine (30mg/kg) group (n=14)③of mtDNA(15ug/ml)+of PDTC (30mg/kg) group (n=14)④of mtDNA (15ug/ml)+PDTC(120mg/kg) group (n=14). Two parts, respectively, in1hour,2hours,4hours,8hours,12hours,24hours and48hours observed by enzyme-linked immunosorbent assay(ELISA) in peripheral blood TNF-alpha, IL-10, IL-6, the expression level;Multi-channel physiological detector to check vital signs in rats;real-time quantitativePCR (Real-time PCR) detection of lung tissue NF-κB subunit p65mRNA, I-κBmRNA,TLR9mRNA expression; fluorescence quantitative PCR detection of peripheral bloodmtDNA levels; HE staining of liver changes in the pathology of the lung, kidney,intestine. The experimental data were presented as mean±standard error (x±s)represents. All data use SPSS17.0statistical software using single-factor analysis ofvariance (one way ANOVA) and regression and correlation analysis, P <0.05significantdifference.Results Between the groups in rat blood pressure and breathing did not changesignificantly in all experimental groups, significant changes in body temperature atdifferent points in time, but the temperature change between the groups was notstatistically significant.Buffer injection in rats and nDNA rats no significant changes ateach time point of peripheral blood levels of inflammatory factors; mitochondrialfragmentation and mtDNA groups began to rise in one hour levels of inflammatoryfactors, and eight hours to reach the peak, with the injection of mitochondrialfragmentation andmtDNA concentration, the higher the level of inflammation, andmitochondrial fragments containing the corresponding mtDNA concentration with highlevels of inflammatory factors than this concentration of mtDNA.p65mRNA, I-κBmRNA expression levels in lung tissue and inflammatory factors, TLR9expressionlevels increased linearly related.p65mRNA, I-κB mRNA expression levels in lung tissuenegatively correlated.Chloroquine and PDTC the higher the concentration, the lower the level of expression of inflammatory cytokines to block the better, but chloroquine blockthe effect of PDTC was no significant difference.Conclusions mtDNA can stimulate TLR9-induced NF-κB pathway leading to thebody’s systemic inflammatory response syndrome. The protective effect of chloroquineand PDTC can be caused by mtDNA SIRS.

Related Dissertations

  1. Variations of Host-Specialized and Migratory Biotypes of Cotton Aphid on the Mating Behavior and Genes Sequences of Mitochondrion DNA and Symbiotic Bacteria,S433
  2. Phylogenic Analysis of Henan Populations and Feeding Behavior Comparison of Bemisia Tabaci B and Q Biotypes,S433
  3. Effects of Endosymbionts Wolbachia on mtDNA Variation and Evolution in Natural Populations of Tetranychu Urtica Koch (Acari: Tetranychidae) from China,S433.7
  4. The Effects of PCV2 on NF-κB Signal in Piglet’s Lymphocytes in Vitro,S858.28
  5. Nuclear Dot Protein NDP52 Bind to Tumor Necrosis Factor Receptor Associated Factor 6 and Their Clinical Significance of Research,R363
  6. Effective of the Interaction between NDP52 and TRAF6 and TRAFs on NF-κB Signal Pathway,R363
  7. The Molecular Mechanism Intervention of Selenium on the Learning and Memory Injury by Drinking Water Fluorosis,R599
  8. The Sequence Analysis of RNA Components from Lactobacillus Metabolites and Parts of the Biological Function,R378
  9. Effects of Hypothermia Resuscitation on PPAR-γ and Related Gene Expression in Rats Lung during Hemorrhagic Shock,R605.971
  10. The Study of the Influence of BMS-345541 on Motor Function of Hind Limbs and the Infiltrating Leukocytes after Spinal Cord Injury in Rats,R651.2
  11. Influence on NF-κB P65 and ASPP2 of Murine Lewis Lung Cancer of NF-кB Inhibitor PDTC and Cisplatin,R734.2
  12. Restructuring Agkistrodon disintegrin Adinbitor human non-small cell lung cancer cell line A549,R285.5
  13. NF-κB inhibitor PDTC on non-small cell lung cancer cells and JNK protein expression iASPP impact,R734.2
  14. Danxingtongluo decoction on cerebral ischemia-reperfusion injury in TNF-α, NF-κB expression,R285.5
  15. The Initial Impact Research of Vagotomy and Nicotine Pretreatment on of Rats with SIRS,R363
  16. The Experimental Studies of the Protection of α-lipoic Acid Against Lung Injury Induced by Acute Necrotic Pancreatitis in Rats,R563.8
  17. Inhibition of NF-κB Contribute to Autophagy and Affect Cell Death in Intracerebral Hemorrhage Mice,R743.3
  18. Effects of Propofol on the Apoptosis and Activation of NF-κB in Polymorphonuclear Neutrophil in Acute Lung Injury Induced by LPS in Rats,R965
  19. The Study of the Mechanism of PARP-1 in LPS Induced Inflammation in Rat Cardiomyocyte,R363
  20. The Involvement of NF-κB Subunits in the Interaction Between CLL-B Cells and Bone Marrow Stromal Cells,R733.7
  21. The Superficial Masseter Muscle Cells Apoptosis after the Function Mandibular Advancement in Juvenile Rats,R783.5

CLC: > Medicine, health > Clinical > Therapy > Emergency, first aid
© 2012 www.DissertationTopic.Net  Mobile