Dissertation > Excellent graduate degree dissertation topics show

3,3’-Diindolymethane Ameliorates Adriamycin-induced Cardiac Fibrosis Via Activation of a BRCA1-dependent Anti-oxidant Pathway

Author: YaoZhengWei
Tutor: ZhangJunFeng
School: Nanjing University
Course: Pharmaceutical Engineering
Keywords: adriamycin cardiac fibrosis DIM BRCA1 anti-oxidation
CLC: R96
Type: Master's thesis
Year: 2013
Downloads: 41
Quote: 0
Read: Download Dissertation

Abstract


Adiramycin(ADR)that is a broad-spectrum chemotherapitic drug is used to treat with various of cancers.However, adriamycin and other anthracycline derivatives is limited due to dose-dependent cardiotoxicity. One of the most recognized toxicities of anthracycline derivatives is their strong promotion of oxidant formation. The oxygen radical that is induced by adriamycin could cause inflammation response which subsequently promotes cardiac fibrosis. Cardiac fibrosis is the main cause of adriamycin-induced toxicity.In our present studies, we observed the histopathologic change using HE staining; measured the disposition of collagen I in cardiac tissue by sirus red staining; assessed the production of BRCA1protein in cardiac tissue and fibroblast using western blotting and immunofluorescence; evaluated the expression level of a-SMAx collagen I、GPX3、GST mRNA using Q-PCR; estimated the content of ROS in fibroblast. The results were shown that DIM could reduce the level of α-SMA、collagen I in cardiac tissue and fibroblast. Additionally, we also proved that the level of BRCA1, GPX3and GST mRNA was markedly increased in mice that treated with adriamycin and DIM compared with that of mice administrated adriamycin alone. ROS that was induced by adriamycin in fibroblast was significantly suppressed by DIM. The above results suggested DIM could reverse adriamycin-induced cardiac fibrosis via a brcal-dependent anti-oxidant pathway.

Related Dissertations

  1. Studies on Extraction, Isolation, Purification and Antioxidation Activity of Flavonoids from the Fruits of Avicennia Marina,S793.9
  2. Based on the tutor clinical experience in medical treatment of chronic kidney disease proteinuria,R277.5
  3. The Study on Anti Tumor and Anti-oxidation Activities in Vitro of Extracts from Oxalis Corniculata L,R285
  4. Research on Biological Modification and Protection Activity of R-Phycoerythin from Porphyra Yezoensis,TS201.21
  5. Quantifying the Response of the Grain Quality of Wheat to DIM Light at Filling Stage,S512.1
  6. Experimental Study of GUI QIN MISTURE on Adriamycin-induced Nephropathy in Rats,R285.5
  7. The Molecular Biology of Rh-endostatin Plus Chemotherapy as Adjuvant Treatment for Non-small Lung Cancer,R734.2
  8. Expression and Prognostic Significance of TS and BRCAl mRNA in Non-small Cell Lung Cancer,R734.2
  9. Establishment of Nude Mousemodels of MG63 and MG63/ADR Osteosarcoma Cell Lines and the Significance of Expression of VEGF and NF-κB1,R738.1
  10. The Effects of Liyu Chiciaodou Tang on Expression of Aquaporin 2 and 3 in Adriamycin Nephrosis Rats,R285.5
  11. Methylation of the BRCA1 gene and SNP and sporadic breast Correlation,R737.9
  12. Research on Dim Small Targets Detection in Infrared Images,TP391.41
  13. Effects of Cyprinus Carpio Decoction on the Expression of Aquaporin-1, Aquaporin-2 in Adriamycin Nephrosis Rats,R285.5
  14. Experimental Study on Apoptosis Inducted by (-)-Gossypo with Combination of Low Concentration of Adriamycin in Sof t Tissue Sarcoma Cells and Its Mechanisms,R965
  15. Correlative Research of Expression of ERCC1,BRCA1,Ki-67 and Outcome of CEF Neoadjuvant Chemotherapy of Breast Cancer,R737.9
  16. Chinese Pd aqueous extract of doxorubicin in rats pharmacokinetics and tissue distribution of,R285.5
  17. Ginger decoction intervention doxorubicin - induced cardiac toxicity injury in rats pharmacodynamics,R285.5
  18. Association between MGMT and BRCA1 Gene Methylation and DNA Damage in Peripheral Blood Lymphocytes among Nickel Workers,R131
  19. The Expression of BRCA1 in Basal-like Breast Cancer and Its Relationship with ID4,R737.9
  20. Expression and Clinical Significance of BRCA1 and BRCA2 in Basal-like Breast Carcinoma,R737.9
  21. Expression ERCC1, and BRCA1 in 152 Cases of Advanced non Small Cell Lung Cance and Their Clinical Significance,R734.2

CLC: > Medicine, health > Pharmacy > Pharmacology
© 2012 www.DissertationTopic.Net  Mobile